Anti irf1 h 205

Manufactured by Santa Cruz Biotechnology

The Anti-IRF1 H-205 is a rabbit polyclonal antibody that recognizes the interferon regulatory factor 1 (IRF1) protein. IRF1 is a transcription factor that plays a role in the regulation of interferon-stimulated genes and other cellular processes. The antibody is suitable for applications such as Western blotting, immunoprecipitation, and immunohistochemistry.

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Lab products found in correlation

Mg132, by Thermo Fisher Scientific (1 mentions) Anti isg15 f 9, by Santa Cruz Biotechnology (1 mentions) Anti flag m2, by Merck Group (1 mentions) Human ifnα, by PBL Assay Science (1 mentions) Trimethoprim tmp, by Merck Group (1 mentions) Anti phosphorylated stat2 tyr690, by Cell Signaling Technology (1 mentions) Anti gapdh 6c5, by Santa Cruz Biotechnology (1 mentions) Anti irf3, by Santa Cruz Biotechnology (1 mentions) Recombinant irf1 protein, by Abcam (1 mentions)

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Anti-IRF1 (10 citations)

2 protocols using anti irf1 h 205

Interferon Signaling Pathway Modulation

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Rhesus IFNα2 was obtained from R&D systems. Human IFNα and universal type I IFN (uIFN) were obtained from PBL Assay Science. MG132 (Fisher Scientific) was dissolved in DMSO and used at the indicated concentrations for 16 hours. Control wells were treated with same concentration of DMSO without MG132. Trimethoprim (TMP; Sigma-Aldrich) was dissolved in DMSO and used 10 μM or less where indicated. Viral cultures were supplemented with fresh TMP every 24 hours. The following antibodies were used for detection of endogenous and viral proteins in western blot: anti-ISG15 F-9 (Santa Cruz), anti-ISG54/IFIT2 (Abcam), anti-Mx-1 (GeneTex), anti-STAT1 M22 (Santa Cruz), anti-phosphorylated STAT1 Tyr701 (Santa Cruz), anti-STAT2 C20 (Santa Cruz), anti-phosphorylated STAT2 Tyr690 (Cell Signaling Technology), anti-IRF9/ISGF3γ clone 6 (BD Biosciences), anti-GAPDH 6C5 (Santa Cruz), anti-p84 5E10 (GeneTex), anti-IRF1 H-205 (Santa Cruz), anti-IRF3 (Santa Cruz) and anti-FLAG M2 (Sigma-Aldrich). The monoclonal antibodies specific for SVV and VZV ORF63 (clone 63_6), ORF62 (clone 62_6) and ORF31 (clone 31C_8) have been previously described [35 (link)]. STAT2 C20 (Santa Cruz) was also used for immunofluorescence microscopy.

Verweij M.C., Wellish M., Whitmer T., Malouli D., Lapel M., Jonjić S., Haas J.G., DeFilippis V.R., Mahalingam R, & Früh K. (2015). Varicella Viruses Inhibit Interferon-Stimulated JAK-STAT Signaling through Multiple Mechanisms. PLoS Pathogens, 11(5), e1004901.

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Transcription Factor IRF1 Binding Assay

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Radiolabeled ³²P oligonucleotides were added to THP-1 nuclear extracts or recombinant IRF1 protein (Abcam) in a binding buffer solution (10mM Tris-HCl pH 7.5, 53mM NaCl, 1mM DTT, 0.01% Nonidet-P40, 5% glycerol, and 0.05ug/ul of double-stranded poly(dI-dC)) at RT for 30min. In super-shift experiments, samples were incubated with 2ug of anti-IRF1 (H-205, Santa Cruz Biotechnology). Protein-DNA complexes were separated by electrophoresis on a 5% PAAG gel.

Chow N.A., Jasenosky L.D, & Goldfeld A.E. (2014). A distal locus element mediates IFN-γ priming of LPS-stimulated TNF gene expression. Cell reports, 9(5), 1718-1728.

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