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Magnesium chloride anhydrous

Manufactured by Merck Group
Sourced in Germany, Italy

Magnesium chloride anhydrous is a chemical compound with the formula MgCl2. It is a white, crystalline solid that is highly soluble in water and other polar solvents. The primary function of magnesium chloride anhydrous is to serve as a laboratory reagent and a source of magnesium ions in various chemical reactions and analyses.

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8 protocols using magnesium chloride anhydrous

1

Chitosan-Polyphosphate Biomaterials Synthesis

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Chitosan (Cs) 25 kDa was purchased from Heppe Medical Chitosan GmbH (Halle, Germany) and sodium polyphosphate (PP) from Merck KGaA (Vienna, Austria). Alkaline phosphatase from bovine intestinal mucosa (7165 units/mg protein), ammonium molybdate tetrahydrate (8–83%), glucose-D-(+) ≥ 99.5% anhydrous, magnesium chloride anhydrous (MgCl2) ≥ 98%, minimum essential medium (MEM) eagle, malachite green oxalate salt 90%, potassium phosphate monobasic (KH2PO4) ≥ 99.5%, phosphatase inhibitor cocktail 2, resazurin sodium salt, rhodamine 123, Triton X-100, and anhydrous zinc chloride (ZnCl2) ≥ 97% were purchased from Sigma-Aldrich (Vienna, Austria). 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) ≥ 99.5% was purchased from ROTH GmbH (Karlsruhe, Germany). Fetal bovine serum (FBS) and phosphate-buffered saline (PBS) were purchased from Biochrom, Merck (Tutzing, Germany). D-(+)-Trehalose dehydrate ≥98.0% was purchased from TCI Chemicals (Eschborn, Germany).
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2

Synthesis of Luminescent Coordination Polymers

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Silver
nitrate (AgNO3), calcium chloride dihydrate (CaCl2·2H2O), sodium nitrate (NaNO3), potassium
chloride
(KCl), manganese chloride (MnCl2), copper II chloride dihydrate
(CuCl2·2H2O), magnesium chloride anhydrous
(MgCl2), aluminum chloride (AlCl3), iron III
chloride (FeCl3), dysprosium III chloride hexahydrate (DyCl3·6H2O), sodium citrate tribasic tetrahydrate
(HOC(COONa) (CH2COONa)2·4H2O),
hydrogen peroxide (H2O2), terbium III chloride
hexahydrate (TbCl3·6H2O), ytterbium III
chloride hexahydrate (YbCl3·6H2O), holmium
III chloride hexahydrate (HoCl3 ·6H2O),
lead chloride (PbCl2), sodium carbonate anhydrous (Na2CO3), sodium phosphate (Na3PO4), sodium sulfate (Na2SO4), sodium fluoride
(NaF), sodium acetate (CH3COONa), disodium tartrate, disodium
succinate, disodium malate, CTAB, polyethylene glycol 1500, and dodecanoic
acid were purchased from Sigma-Aldrich Company (South Korea). Fumaric
acid, p-toluene sulfonic acid (PTSA), and 3-(4-hydroxyphenyl)propanoic
acid were purchased from Merck (Germany). All solvents and chemicals
were used as received without purification. DI water filtered to 18
MΩ·cm was used in all experiments.
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3

Trace Metal Preparation Protocol

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Ascorbic acid (C6H8O6), ethylene glycol (C2H6O2), magnesium chloride anhydrous (MgCl2), manganese(II) chloride tetrahydrate (MnCl2·4H2O), iron(II) chloride anhydrous (FeCl2), cobalt(II) chloride hexahydrate (CoCl2·6H2O), nickel(II) chloride hexahydrate (NiCl2·6H2O), ammonium oxalate monohydrate [(NH4)2C2O4·H2O], and manganese dioxide (MnO2) were all obtained from Sigma-Aldrich. Deionized ultrapure water with a resistivity of 18.2 megohm⋅cm was used for all experiments.
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4

Electrochemical Detection of Furanone Compounds

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4-Hydroxy-2,5-dimethyl-3(2H)-furanone, 4,5-dimethyl-3-hydroxy-2,5-dihydrofuran-furanone (sotolon), 5-ethyl-4-hydroxy-2-methyl-3(2h)furanone (homofuraneol), 5-ethyl-3-hydroxy-4-methyl-2(5h)-furanone (maple furanone), magnesium chloride anhydrous, hydrochloride acid, Tris, sodium chloride, cysteamine, N(3-dimethylaminopropyl)N ethylcarbodiimide (EDC), N-Hydroxysulfosuccinimide sodium salt (NHS), 1,8-Octanedithiol, N-Hydroxy succinimide (NHS), ethanol, 11-mercaptoundecanoic acid (11-MUA), phosphate buffer saline (PBS), potassium chloride (KCl), potassium ferricyanide III (K3[(Fe(CN)6]), potassium ferricyanide II trihydrate (K4[(Fe(CN)6]x3H2O), Celite 545, MWCNT carboxyl, sodium dodecyl sulphate (SDS) were obtained from Sigma Aldrich (Munich, Germany). Furaneol aptamer (5′-amine group -CGCCAGCTCATTCCTCACCACGAGAAAGGAGCTCGATGAACTGCGAGCCGGATTCGACCCTATGCGAGTAGGTGGT- methylene blue-3′) amine group at the 5′-end was ordered from Microsynth AG (Balgach, Switzerland) [19 (link)]. Polyethylene Terephthalate (PET) flexible substrate with a 125-micron thickness was purchased from Mylar (Chester, VA, USA). Ink pastes, silver chloride ECI 6038E and silver ECI 1011, were purchased from LOCTITE E&C (CA, USA). All chemicals used in this work are analytical grade and were used without any further purifications.
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5

Phosphatase Assay Protocol for Cell Cultures

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Sodium polyphosphate [PolyP, Graham's salt], PEI [molecular mass (mm) 25 kDa, branched], alkaline phosphatase from bovine intestinal mucosa (7165 units/mg protein), magnesium chloride anhydrous (MgCl2, 98%), zinc chloride anhydrous (ZnCl2, 97%), potassium phosphate monobasic (KH2PO4, 99.5%), phosphatase inhibitor cocktail 2, Triton X-100, ammonium molybdate tetrahydrate (81-83%), malachite green (MLG) oxalate salt (90%), fluorescein diacetate (FDA), minimum essential medium (MEM) eagle, glucose-d-(+) ≥99.5% (anhydrous), and resazurin sodium salt were purchased from Sigma-Aldrich (Vienna, Austria).
Fetal bovine serum (FBS), penicillin-streptomycin liquid and phosphate-buffered saline (PBS) were purchased from Biochrom, Merck (Tutzing, Germany). d-(+)-Trehalose dihydrate >98.0% (GC) was purchased from TCI Chemicals (Eschborn, Germany). 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) ≥99.5% was obtained from ROTH GmbH (Karlsruhe, Germany).
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6

Polyelectrolyte-Based Ion-Exchange Membrane Preparation

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Poly(allylamine
hydrochloride) (PAH, Mw = 17 500
Da), poly(sodium 4-styrenesulfonate)
(PSS, Mw = 70 000 Da), sodium chloride
(NaCl, ≥99%), anhydrous magnesium chloride (MgCl2, ≥98%), and sodium 2-mercaptoethanesulfonate (MESNA, analytical
standard, ≥ 98.0) were purchased from Sigma-Aldrich. Hydrochloric
acid (36.5–28.0% NF grade) was purchased from VWR International.
Nitric acid (65%, for analysis) and ethanol (absolute) were purchased
from Merck Millipore. Acetone (HPLC grade, 99.9%) and dichloromethane
(stabilized with amylene) were purchased from BIOSELVE BV. All chemicals
were used as received without further purification. The inorganic
salts were kept in a vacuum oven overnight prior to use.
Neosepta
cation-exchange (CMX), anion-exchange (AMX), and monovalent cation-selective
(CIMS) membranes (Astom Corp., Japan) were soaked in a solution of
4 mM NaCl and 4 mM MgCl2 for at least 48 h before use.
The porous carbon electrodes, which were deposited on a graphite foil
substrate, were supplied by Voltea BV, Netherlands. Glass fiber prefilters
(25 mm in diameter) with a pore size of 2.0 μm (Merck Millipore)
were used as a spacer for MCDI experiments. Flat substrates of gold
sputtered on glass (1 × 1 cm) were purchased from ECsens. Milli-Q
water (18.2 MΩ·cm, Milli-Q Integral 3 system, Millipore)
was used to prepare salt and polyelectrolyte solutions.
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7

Antioxidant Capacity Evaluation

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Folin and Ciocalteu’s phenol reagent, sodium carbonate (≥99.5%), 2,2′-diphenyl-1-picrylhydrazyl (95%) (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (97%; Trolox), citric acid (≥99.5%), and anhydrous magnesium chloride (≥98%) were purchased from Sigma-Aldrich (Milan, Italy). Gallic acid and ethanol (≥99.9%) were supplied by Fluka (Milan, Italy). Ultrapure water was prepared using a Milli-Q filter system (Millipore, Milan, Italy).
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8

Analytical-Grade Chemical Preparation

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The chemicals, such as analytical grade anhydrous calcium chloride (CaCl2), anhydrous magnesium chloride (MgCl2), anhydrous sodium carbonate (Na2CO3), dipotassium phosphate (K2HPO4), and potassium dihydrogen phosphate (KH2PO4), were purchased from Sigma-Aldrich without further purification.
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