The human prostate cancer cells 22Rv1 and human prostate non-cancer cells PNT-2 were purchased from ATCC. Cell lines were cultured according to the manufacturers instructions in 10% FBS/RPMI media (Invitrogen). Cells were continuously kept in culture for a maximum of 3 months, and were routinely inspected microscopically for stable phenotype and regularly checked for contamination with mycoplasma. The authentity of the cells has recently been confirmed by the Multiplexion (Heidelberg, Germany) using highly polymorphic short tandem repeat loci.
Fbs rpmi media
Manufactured by Thermo Fisher Scientific
Sourced in United States, United Kingdom
FBS/RPMI media is a cell culture medium designed to support the growth and maintenance of a variety of cell lines. It consists of a balanced salt solution supplemented with fetal bovine serum (FBS) and the RPMI 1640 formulation. This media provides the necessary nutrients, growth factors, and other components required for cell proliferation and survival in in vitro cell culture applications.
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Lab products found in correlation
6 protocols using fbs rpmi media
1
Cell Line Authentication and Maintenance
2
Culturing of Prostate Cell Lines
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The human prostate cancer cells 22Rv1 and human prostate non-cancer cells PNT2 were purchased from ATCC. Cell lines were cultured according to the manufacturer’s instructions in 10% FBS/RPMI media (Invitrogen, Carlsbad, CA, USA) and handled as described in [29 (link)].
3
Prostate Cancer Cell Line Cultivation
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The human prostate cancer cells lines 22Rv1, PC-3, and LNCaP were purchased from ATCC. Cell lines were cultured according to the manufacturers instructions in 10% FBS/RPMI media (Invitrogen) with (for LNCaP) or without (for 22Rv1 and PC-3) 1 mM sodium pyruvate (Invitrogen). Cells were continuously kept in culture for a maximum of 3 months and were routinely inspected microscopically for stable phenotype and regularly checked for contamination with mycoplasma. Cell line authentication was performed by DSMZ (Braunschweig, Germany) using highly polymorphic short tandem repeat loci [15 (link)].
4
Culturing Prostate Cancer Cell Line PC-3
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The human prostate cancer cells line PC-3 was purchased from ATCC. Cells were cultured according to the manufacturer’s instructions in 10% FBS/RPMI media (Invitrogen, Paisley, UK) containing penicillin/streptomycin (Invitrogen). Cells were continuously kept in culture for a maximum of three months, and were routinely inspected microscopically for stable phenotype and regularly checked for contamination with mycoplasma. Cell line authentication was recently performed by DSMZ (Braunschweig, Germany) using highly polymorphic short tandem repeat loci [18 (link)].
5
Culturing Prostate and Neuroblastoma Cell Lines
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The human prostate cancer cell lines 22Rv1, PC-3, and LNCaP and the murine neuroblastoma cell line Neuro-2a were purchased from ATCC.
22Rv1, PC-3, and LNCaP cell lines were cultured according to the manufacturer’s instructions in 10% FBS/RPMI media (Invitrogen, Carlsbad, CA, USA). Cells were continuously kept in culture for a maximum of 3 months and were routinely inspected microscopically for stable phenotypes and regularly checked for contamination with mycoplasma. Cell line authentication was performed by DSMZ (Braunschweig, Germany) using highly polymorphic short tandem repeat loci [42 (link)].
Neuro-2a cells were cultured in DMEM medium containing 10% fetal bovine serum (Biolot, St. Petersburg, Russia) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA). Cells were incubated at 37 °C in a humidified atmosphere containing 5% (v/v) CO2 [43 (link)].
22Rv1, PC-3, and LNCaP cell lines were cultured according to the manufacturer’s instructions in 10% FBS/RPMI media (Invitrogen, Carlsbad, CA, USA). Cells were continuously kept in culture for a maximum of 3 months and were routinely inspected microscopically for stable phenotypes and regularly checked for contamination with mycoplasma. Cell line authentication was performed by DSMZ (Braunschweig, Germany) using highly polymorphic short tandem repeat loci [42 (link)].
Neuro-2a cells were cultured in DMEM medium containing 10% fetal bovine serum (Biolot, St. Petersburg, Russia) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA). Cells were incubated at 37 °C in a humidified atmosphere containing 5% (v/v) CO2 [43 (link)].
6
Culturing Human Prostate Cancer Cell Lines
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The human prostate cancer cells lines 22Rv1, PC-3, and LNCaP were purchased from ATCC. Cell lines were cultured in 10% FBS/RPMI media (Invitrogen Ltd., Paisley, UK) with (for LNCaP) or without (for 22Rv1 and PC-3) 1 mM sodium pyruvate (Invitrogen). Cells were continuously kept in culture for a maximum of 3 months, and were routinely inspected microscopically for stable phenotype and regularly checked for contamination with mycoplasma. Cell line authentication was performed by DSMZ (Braunschweig, Germany) using highly polymorphic short tandem repeat loci [30 (link)].
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