Spss ver 17

SPSS ver. 17.0 software (SPSS Inc., Chicago, IL, USA) was used for data analysis. All data are presented as the mean ± standard deviation. Groups were compared using single-factor analysis of variance (ANOVA). Pairs of groups were compared using the least significant difference test. A p-value < 0.05 was considered significant.

MZmine 2.0 software (14 (link)) was used for peak detection, alignment and normalization. The filter conditions were: Each chromatography peak signal-to-noise ratio >30, the retention time tolerance at ± 0.1 min and the m/z tolerance at ±0.01 Da. SIMCA-P+ ver. 12.0.1.0 software (Umetrics, Malmö, Sweden) was used to establish the principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) model of all the samples and the result was checked by cross validation described previously (15 (link),16 (link)). Preliminary selection of characteristic metabolites was accomplished using the corresponding variable influence on projection (VIP) value, confidence interval and coefficient plot generated by the OPLS-DA model. SPSS ver. 17.0 software (SPSS Inc., Chicago, IL United States) was used to evaluate the statistical significance of differences of the variances among diverse groups. The ROC curves were generated and the corresponding AUC was calculated. P<0.05 was considered to indicate a statistically significant difference.

The SPSS ver. 17.0 software (SPSS Inc., Chicago, IL, USA) was used for statistical analysis. Results are presented as mean±standard deviation values and frequency as numbers (in percent). The Z-test for proportions was used to compare the proportions between the two groups. A p-value of ≤0.05 was considered as statistically significant.

The data analysis was performed using SPSS ver. 17.0 software (SPSS Inc., Chicago, IL) and GraphPad Prism 6.0 software (GraphPad Software Inc., San Diego, CA). Group comparisons for continuous data were done by t test or oneway ANOVA. Biochemical experiments were performed in triplicate and a minimum of three independent experiments were evaluated. The value of p < 0.05 was considered statistically significant.

Chi-square and independent t tests were performed to determine the differences between two groups in terms of sex, age, lesion side, days to MEP, and hemorrhage volume. Mann-Whitney test was used to compare MI scores between at onset and at six months after onset or changes of MI score for six months of the two groups. Chi-square test was performed to compare the distribution of prognosis between early MEP and late MEP groups. SPSS ver. 17.0 software (SPSS Inc., Chicago, IL, USA) was used for all statistical analyses. Statistical significance was considered when p-value was less than 0.05.

After staining, slides were observed under Epifluorescent microscope for scoring. Magnification power was adjusted to 40× and 50 cells were counted for each slide. Computer image analysis Casplab® software was used for scoring of cells and various parameters including Length Tail, Tail DNA and Olive Tail Movement. SPSS® (Ver. 17.0) software was used to statistically analyze the results. One Way ANOVA was used for all comparison of comet parameters and Post Hoc Test was used for the comparison of significant values of various parameters within groups and between groups.