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3 protocols using tocopheryl acetate

1

Carotenoid and Vitamin E Quantification

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Lutein (xanthophyll from marigold), zeaxanthin, α- and β-carotene, β-cryptoxanthin, lycopene and phytoene, tocopheryl acetate, trimethylamine, celite, potassium hydroxide (KOH), phosphate buffered saline (PBS) and sodium chloride (NaCl) were obtained from Sigma Aldrich (Madrid, Spain). Sodium sulphate anhydrous and pyrogallic acid were supplied by Panreac (Barcelona, Spain). Hexane (Hex), methyl tert-butyl ether (MTBE), methanol (MeOH), ethanol, dichloromethane (DCl), petroleum ether (PE) and diethyl ether (DE), were obtained from Análisis Vínicos (Spain). MeOH and MTBE were HPLC grade.
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2

Carotenoids and Tocopherols Extraction

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Lutein (xanthophyll from marigold), zeaxanthin, α- and β-carotene, β-criptoxanthin and phytoene, tocopheryl acetate, trimethylamine, celite, potassium hydroxide (KOH) and sodium chloride (NaCl) were obtained from Sigma Aldrich (Madrid, Spain). Anhydrous sodium sulfate and pyrogallic acid were supplied by Panreac (Barcelona, Spain). Methyl tert-butyl ether (MTBE), methanol (MeOH), ethanol, dichloromethane, petroleum ether and diethyl ether, were obtained from Análisis Vínicos (Spain). MeOH and MTBE were High-Performance Liquid Chromatographic (HPLC) grade.
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3

Quantification of Lipophilic Vitamins

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Standards of retinol, α-tocopherol, β-carotene, lycopene, lutein, and tocopheryl acetate were purchased from Sigma (St. Louis, MO, USA). HPLC gradient grade methanol, ethanol, acetonitrile, metylene chloride, and n-hexane were obtained from Merck (Merck KGaA, Darmstadt, Germany).
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