Jsm 6360 la microscope

The green-synthesized ZnO-NPs were characterized through different instrumental techniques. Scanning electron microscopy (SEM) was applied to elucidate the surface morphology and shape of the prepared ZnO-NPs using a JSM-6360 LA microscope (JEOL, Tokyo, Japan). An XRD-7000 (Shimadzu, Kyoto, Japan) diffractometer with a CuK radiation beam (λ = 0.154060 nm), 30 kV and 30 mA, and 10–80° in 2θ was used to generate X-ray diffraction (XRD) patterns. A UV–visible spectrophotometer (Shimadzu, Tokyo, Japan) was used to look for the ZnO-NPs. The reduction of Zn+ ions was confirmed by measuring at the UV-245 double beam (300–800 nm). The functional groups in the prepared ZnO-NPs were evaluated in the range of 400–4000 cm⁻¹ through Fourier transform infrared spectroscopy (FTIR) using 8400 s Shimadzu FTIR (Japan) and the KBr-disc method. Furthermore, the Zetasizer ver. 6.2 (ZS, Malvern, Kassel, Germany) was applied to investigate the particle-size distribution, polydispersity index, and net surface charge (zeta-potential) of the prepared ZnO-NPs.

SPE procedures were carried out using a Visi-1 Single SPE Tube Processor purchased from Sigma-Aldrich (Germany). pH measurements were done using Jenway pH meter 3310 (UK). Ultrapure water was prepared using the Stakpure Pure water system OMNIATYPE1, Stakpure GmbH (Germany). Fourier transform infrared (FTIR) spectra were recorded using Thermo Scientific™ Nicolet™ iS50 FTIR spectrometer (Germany). The surface area, pore volume, and pore size of the polymers were calculated using Brunauer–Emmett–Teller (BET) and Barret-Joyner-Halenda (BJH) methods via NOVAtouch® LX4 Quantachrome (USA). Surface morphology was examined using the JEOL JSM-6360LA microscope for field-emission scanning electron microscopy (SEM) (Japan). All the analytical measurements were carried out on ACQUITY UPLC H-Class system that is composed of Xevo™ TQD triple-quadrupole tandem mass analyzer with an electrospray ionization (ESI) interface, Mass Lynx 4.1 software, and target Lynx quantification program, all provided by Waters Corp. (USA). Acquity UPLC BEH C18 column (1.7 µm, 100 mm × 2.1 mm) was used to separate the target analytes (Waters, Wexford, Ireland).

Different experimental approaches were used to characterize the green synthesized FS-Ag-NPs as follows: the surface-morphological structures of the produced FS-Ag-NPs were examined through scanning electron microscopy (SEM) using the JSM-6360 LA microscope (JEOL, Tokyo, Japan). The shape of the particles was determined through transmission electron microscopy (TEM, JSM-6360, JEOL, Tokyo, Japan), where the elemental analysis of FS-Ag-NPs was conducted through the energy-dispersive X-ray spectroscopy (EDX) unit combined with TEM. Furthermore, Fourier-transform infrared spectroscopy (FTIR, Agilent Technologies, Inc., Santa Clara, CA, USA) was used to evaluate the functional groups on the prepared FS-Ag-NPs using the KBr-disc approach. The zeta potential for prepared FS-Ag-NPs was elucidated through Zetasizer ver. 6.2 (ZS, Malvern, Germany).