Transwell 24 well insert pore size 8 μm

Manufactured by Corning

The Transwell (24-well insert; pore size 8 μm) is a laboratory equipment product designed for cell culture applications. The core function of this product is to facilitate the study of cell migration, permeability, and other cellular processes that involve the movement of cells or substances across a membrane. The product consists of a 24-well insert with a polycarbonate membrane that has a pore size of 8 μm.

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Transwell chambers (24-well insert; 8-μm pore size; 24-well transwell inserts (8 μm pore; Pore size transwell inserts 24-well transwell inserts 8-μm transwell inserts Transwell inserts (8 μm) 24-well insert 24-well transwell 8-μm pores Transwell inserts

2 protocols using transwell 24 well insert pore size 8 μm

Transwell Invasion Assay Protocol

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Transwell (24-well insert; pore size 8 μm, Corning) polycarbonate filters (6.5 μm) were coated with 50 μL Matrigel. Cells were resuspended in serum-free media and seeded in the upper chamber (5 × 10⁴ cells/200 μL/chamber). The lower chambers were filled with 500 μL of serum-containing media. After culturing for 48 h, noninvading cells on the upper surface of the membrane were removed using a cotton swab and cells on the lower surface were stained with 1% crystal violet. Five randomly chosen areas were photographed under a microscope and the absorbance was measured at 590 nm was measured after dissolving the crystal violet with 10% acetic acid.

Yun E.J., Kim D., Hsieh J.T, & Baek S.T. (2022). Stanniocalcin 2 drives malignant transformation of human glioblastoma cells by targeting SNAI2 and Matrix Metalloproteinases. Cell Death Discovery, 8, 308.

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Matrigel-Based In Vitro Invasion Assay

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In vitro invasion was determined in the Matrigel-based assay. Briefly, 6.5 μm polycarbonate filters of Transwell (24-well insert; pore size = 8 μm; Corning) were coated with 25 μg Matrigel. The lower chambers of Transwell were filled with 600 μl of serum-free medium and the cells were plated in the upper chamber (5×10⁴ cells/200 μl/chamber). After incubation for 48 h, non-invading cells on the upper surface of the membrane were removed by a cotton swab and cells on the lower surface were stained with crystal violet and quantified by measuring OD_560nm with 96-well plate. The cell migration assay was performed with the same method except for Matrigel-coated membrane.

Yun E.J., Zhou J., Lin C.J., Hernandez E., Fazli L., Gleave M, & Hsieh J.T. (2015). Targeting cancer stem cell in castration resistant prostate cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(3), 670-679.

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