Batf3 mice

Manufactured by Jackson ImmunoResearch

Sourced in United States

Batf3−/− mice are a genetically modified mouse strain that lacks the Batf3 gene. The Batf3 gene is involved in the development and function of a specific type of immune cell called the dendritic cell. These mice can be used to study the role of Batf3-dependent dendritic cells in various immunological processes.

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27 protocols using batf3 mice

Genetically Engineered Mouse Models

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Zbtb46^GFP mice were a generous gift from Dr. Kenneth Murphy (Satpathy et al., 2012 (link)). Ccr2^−/− mice were obtained from Jackson Laboratories and bred to Kras^G12D: Trp53^flox mice. LysM^Cre mice were obtained from Jackson Laboratories and bred to 1) Rosa26^LSL-tdT 2) Irf4^flox or 3) Rosa26^Cas9-eGFP mice. Batf3^−/− mice were obtained from Jackson Laboratories and/or bred in house for experimental use.
Sex (both male and female) and aged matched mice between 6–8 weeks were used for these studies. Mice were bred and maintained in specific pathogen free facilities at the University of Pennsylvania. All animal procedures were conducted according to National Institutes of Health guidelines and approved by the Institutional Animal Care and Use Committee at the University of Pennsylvania.

Devalaraja S., Jerrick To T.K., Folkert I.W., Natesan R., Alam M.Z., Li M., Tada Y., Budagyan K., Dang M.T., Zhai L., Lobel G.P., Ciotti G.E., Eisinger-Mathason T.S., Asangani I.A., Weber K., Simon M.C, & Haldar M. (2020). Tumor-Derived Retinoic Acid Regulates Intratumoral Monocyte Differentiation to Promote Immune Suppression. Cell, 180(6), 1098-1114.e16.

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Murine Models for Immunological Research

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C57BL/6J mice were either bred in-house or purchased from Charles River (Germany). p110γ-KD mice were generated and provided by E. Hirsch, University of Torino and were back-crossed to C57BL/6 for at least 15 generations. BATF3-/- mice were obtained from Jackson (USA). All animals were housed in individually ventilated cages under specific pathogen free conditions at the ETH Phenomics Facility (Zurich, Switzerland) and used for experiments at between 6 and 14 weeks of age unless otherwise stated. The number of mice (n) indicated in the figure legends always refers to the number of animals per group.

Nobs S.P., Schneider C., Heer A.K., Huotari J., Helenius A, & Kopf M. (2016). PI3Kγ Is Critical for Dendritic Cell-Mediated CD8+ T Cell Priming and Viral Clearance during Influenza Virus Infection. PLoS Pathogens, 12(3), e1005508.

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Characterization of Immune Cell Populations in Mice

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Female C57BL/6 and Batf3^−/− mice were purchased from the Jackson Laboratories. IRF8^fl/fl, Lyz2-Cre, and Lyz2-Cre-IRF8^fl/fl (IRF8-cKO) on a C57BL/6 (B6; H-2^b) background mice were described elsewhere (31 ). Homozygous IRF8–enhanced GFP (EGFP) mice on a B6 background were generous gift from Dr. H. Morse (National Institutes of Health, Bethesda, MD) and have been described (14 (link), 32 (link)). Batf3^−/−, IRF8-EGFP, IRF8^fl/fl, Lyz2-Cre, and IRF8-cKO mice were bred in-house. All mice were age matched (7–10 wk old) at the beginning of each experiment and kept under specific pathogen-free conditions and housed in the Laboratory Animal Resources. All animal studies were conducted in accordance with and approved by the Institutional Animal Care and Use Committee (IACUC) at Roswell Park Comprehensive Cancer Center.

Patel A., Oba T., Kajihara R., Yokoi T., Abrams S.I, & Ito F. (2021). Multimodal intralesional therapy for reshaping the myeloid compartment of tumors resistant to anti-PD-L1 therapy via IRF8. Journal of immunology (Baltimore, Md. : 1950), 207(5), 1298-1309.

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Mouse Models for Immunological Studies

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BALB/c mice (female or male 6- to 8-week-old) were purchased from Charles River Labs. C57BL/6, and Batf3^−/− mice (female 6- to 8 week-old) were purchased from the Jackson Laboratory. Studies using mice were performed in compliance with the Institutional Animal Care and Use Committee of Weill Medical College of Cornell University. All mice were housed in the facility of the Research Animal Resource Center of Weill Medical College of Cornell University.

Wang Y., Song M., Liu M., Zhang G., Zhang X., Li M.O., Ma X., Zhang J.J, & Huang X.Y. (2021). Fascin inhibitor increases intratumoral dendritic cell activation and anti-cancer immunity. Cell reports, 35(1), 108948.

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Generation of CD11c+ cell-specific GP96 knockout mice

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CD11c⁺ cell–specific GP96-deficient mice (CD11cCre⁺Hsp90b1^fl/fl) and control littermates (CD11cCre^–Hsp90b1^fl/fl) were generated by crossing our Hsp90b1^fl/fl (GP96 is encoded by Hsp90b1) mice (58 (link)) with CD11c-Cre transgenic mice (68 (link)). These GP96-deficient mice were further crossed with MMVT-PyMT transgenic mice (69 (link)) to generate MMVT-PyM-CD11cCre⁺Hsp90b1^fl/fl mice. Batf3^–/– mice were purchased from The Jackson Laboratory (stock no. 013755). All mouse models in this study are on the C57BL/6 background.

Iwanowycz S., Ngoi S., Li Y., Hill M., Koivisto C., Parrish M., Guo B., Li Z, & Liu B. (2021). Type 2 dendritic cells mediate control of cytotoxic T cell resistant tumors. JCI Insight, 6(17), e145885.

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Diverse Mouse Models for Immunological Research

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Mice were maintained in a specific pathogen-free environment. Animal experimentation was carried out under a protocol approved by an Institutional Animal Use and Care Committee. C57BL/6 mice (H-2^b; CD45^.2/.2) were purchased from Taconic. Rag2⁻^/⁻ and 2C TCR transgenic mice were bred in our facility. TCRβ/δ⁻^/⁻ mice were provided by M. Alegre (University of Chicago). CD11c^DTR/GFP B6.FVB-Tg (Itgax-DTR/EGFP) mice and Ifnar⁻^/⁻ mice were provided by X.Y. Fu (University of Texas Southwestern). YET40 B6.129-Il12b^tm1Lky/J mice, B6.SJL-Ptprc^a Pepc^b/BoyJ mice (CD45^.1/.1) and Batf3⁻^/⁻ mice were purchased from Jackson Labs and bred in our facility.

Chen X., Fosco D., Kline D.E, & Kline J. (2017). Calreticulin promotes immunity and type I interferon-dependent survival in mice with acute myeloid leukemia. Oncoimmunology, 6(4), e1278332.

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Mouse Strains and Experimental Procedures

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C57BL/6 mice (H-2^b; CD45.2) and B6.SJL-Ptprc^a/BoyAiTac mice (H-2^b; CD45.1) were purchased from Taconic Biosciences and breed in our facility. Rag2^−/− and 2C TCR transgenic mice (Sha et al., 1988 (link)) were bred in our facility. CD11cDTR/GFP B6.FVB-Tg (Itgax^DTR-EGFP) mice and Batf3^−/− mice were purchased from Jackson Labs and bred in our facility. Mice were maintained in a specific pathogen-free environment. 6–12 week-old mice, age and sex matched whenever possible, were used for experiments. Animal experimentation was carried out under a protocol approved by an Institutional Animal Use and Care Committee at The University of Chicago.

Chen X., MacNabb B.W., Flood B., Blazar B.R, & Kline J. (2021). Divergent fates of antigen-specific CD8+ T cell clones in mice with acute leukemia. Cell reports, 37(6), 109991.

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In Vivo Mouse Experiments

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All in vivo experiments were performed in the following mouse strains: 8-week-old female C57BL/6J mice (the Jackson Laboratory), 8-week-old female BALB/c mice (the Jackson Laboratory), 8-week-old BATF3⁻ mice (the Jackson Laboratory, strain no. 013755), and 8-week-old TLR7⁻ mice (the Jackson Laboratory, strain no. 008380). Experiments were performed in specific pathogen–free animal facilities at the MIT Koch Institute for Integrative Cancer Research. Mice were housed under standard 12-hour light–12-hour dark conditions with ad libitum access to water and chow. All mouse studies were performed according to institutional and National Institutes of Health guidelines for humane animal use and in accordance with the Association for Assessment and Accreditation of Laboratory Animal Care. Protocols were approved by the Institutional Animal Care and Use Committee at MIT.

Bhagchandani S.H., Vohidov F., Milling L.E., Tong E.Y., Brown C.M., Ramseier M.L., Liu B., Fessenden T.B., Nguyen H.V., Kiel G.R., Won L., Langer R.S., Spranger S., Shalek A.K., Irvine D.J, & Johnson J.A. (2023). Engineering kinetics of TLR7/8 agonist release from bottlebrush prodrugs enables tumor-focused immune stimulation. Science Advances, 9(16), eadg2239.

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Murine Allografts for Immunology

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Female 8–10 week old C57BL/6 mice or NCr nude (B6NTac, Taconic) were used for allograft experiments. OT-I transgenic mice (Cat# 003831) and Batf3^−/− mice (Cat# 013755) were purchased from Jackson Laboratory. All mice were bred and maintained in the animal facility of the New York University School of Medicine. All animal procedures were approved by the New York University School of Medicine Institutional Animal Care and Use Committee (IACUC) under protocol numbers IA16–00507 and IA16–01331.

Yamamoto K., Venida A., Yano J., Biancur D.E., Kakiuchi M., Gupta S., Sohn A.S., Mukhopadhyay S., Lin E.Y., Parker S.J., Banh R.S., Paulo J.A., Wen K.W., Debnath J., Kim G.E., Mancias J.D., Fearon D.T., Perera R.M, & Kimmelman A.C. (2020). Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Nature, 581(7806), 100-105.

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Mouse Models for Immunological Studies

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