9cis retinoic acid 9cisra

Manufactured by Merck Group

Sourced in United States, Canada

9cis retinoic acid (9cisRA) is a synthetic retinoid compound. It is a structural isomer of all-trans retinoic acid (atRA), a naturally occurring retinoid. 9cisRA is a research chemical used in laboratory settings to study the biological activities and signaling pathways involving retinoid receptors.

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Retinoic acid

5 protocols using 9cis retinoic acid 9cisra

Vitamin D and Retinoic Acid Receptor Interactions

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1α,25-DihydroxyvitaminD3 (1,25D3) and 9cis retinoic acid (9cisRA) were purchased from Sigma. The rat Nppa single strands DNAs (5′-AGAGGTCATGAAGGACATT-3′ and 5′AATGTCCTTCATGACCTCT-3′) were purchased from Sigma Aldrich and annealed. The MED1 NR2 peptide (NHPMLMNLLKDN) was synthesized by Pascal Eberling (IGBMC peptide synthesis common facility).

Belorusova A.Y., Bourguet M., Hessmann S., Chalhoub S., Kieffer B., Cianférani S, & Rochel N. (2020). Molecular determinants of MED1 interaction with the DNA bound VDR–RXR heterodimer. Nucleic Acids Research, 48(19), 11199-11213.

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Protein Expression Analysis of Epigenetic Modulators

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Valproic acid (VPA), trichostatin-A (TSA) and
9-cisretinoic acid (9-cis-RA) were purchased
from Sigma-Aldrich Chemical Co. (St. Louis, MO). Stock solutions of VPA were
prepared by dissolving in distilled water, TSA and 9-cis-RA in
dimethylsulfoxide (DMSO). All stock solutions were stored at − 80
°C before use. Protein concentrations were determined by Bradford's
reagent (Bio-Rad Laboratories Hercules, CA) using bovine serum albumin (BSA) as
standard protein. Antibodies Erg1/2/3 (C-20) rabbit polyclonal antibody,
Fli-1(C-19) rabbit polyclonal antibody, RARβ (C-19)
rabbit polyclonal antibody, CRABPI/II (H-105) rabbit polyclonal antibody and
β-actin (C-4) mouse monoclonal antibody were
purchased from SantaCruz Biotechnology. Horseradish peroxidase (HRP)-conjugated
anti-mouse, anti-rabbit secondary antibodies and enhanced chemiluminescence
(ECL) reagents, Amer-sham ECL Western Blotting Analysis System were purchased
from GE Healthcare.

Kayarthodi S., Fujimura Y., Fang J., Morsalin S., Rao V.N, & Reddy E.S. (2014). Anti-Epileptic Drug Targets Ewing Sarcoma. Journal of pharmaceutical sciences and pharmacology, 1(2), 87-100.

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Retinoid Signaling Pathway Modulation

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ATRA, RARα agonist BMS753, RXRα agonist LG-100268, RARα antagonist BMS195614, 9-cis-retinoic acid (9cis-RA), and dexamethasone were obtained from Sigma-Aldrich (St. Louis, MO, USA). RXRα antagonist UVI3003, anti-β-actin antibody, and secondary antibodies for western blot were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). RPMI-1640 medium for cell culture was obtained from Welgene Inc. (Gyongsan, Korea). Fetal bovine serum (FBS) was purchased from Invitrogen (Gibco BRL, MD, USA).
ATRA was prepared as a 20 mM stock in dimethyl sulfoxide (DMSO) and stored at -20℃ before being diluted to the desired concentration in medium in each experiment.
Purified anti-CPD antibody (ab184960) used in flow cytometric analysis was purchased from Abcam (Cambridge, MA, USA).

Nguyen H.T.T., & Kim J.Y. (2020). All-trans Retinoic Acid Induces Expression and Secretion of Carboxypeptidase D in THP-1 Cells. Journal of Experimental & Biomedical Sciences/Biomedical Science Letters, 26(4), 256-266.

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HEK293 Cell Culture Protocol

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HEK293 cells were obtained from the European Collection of Cell Culture (ECACC) and maintained at 37ºC, 5% CO 2 in presence of DMEM + 10% FBS, 2mM L-glutamine, 50 units/ml penicillin G and 50g/ml streptomycin. Media and supplements were purchased from GIBCO (Invitrogen, Carlsbad, CA) and 9-cis retinoic acid (9-cis RA) from Sigma.
Bexarotene was synthesized as detailed by Wagner and co-workers [10] .

Lee W.P., Jena S., Rodriguez E.P., O'Donovan S.P., Wagner C., Jurutka P.W, & Thompson P.D. (2015). Distinct functional modes of SUMOylation for retinoid X receptor alpha. Biochemical and biophysical research communications, 464(1).

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Receptor Activation Assay

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TBT chloride, TPT chloride, 9-cis-retinoic acid (9cis-RA) and rosiglitazone (Roz) were from Sigma-Aldrich (T50202; Aldrich 245712; R4643; R2408, respectively). The antibodies used were a rabbit polyclonal antibody against Retinoid X Receptor (RXR ΔN 197: sc-774, Santa Cruz Biotechnology), a mouse monoclonal anti-β-tubulin (Sigma-Aldrich, T4026) and peroxidase-conjugated goat anti-rabbit and antimouse IgG antibodies (Jackson ImmunoResearch).

Giraud-Billoud M, & Castro-Vazquez A. (2019). Aging and retinoid X receptor agonists on masculinization of female Pomacea canaliculata, with a critical appraisal of imposex evaluation in the Ampullariidae. Ecotoxicology and environmental safety, 169.

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