Mice bearing KB tumors were prepared as described above. When the tumor size reached 5–8 mm in diameter, the mice (n = 3/group) were treated with an intravenous injection of FA-[64Cu]CuS NPs (200 μL, 7.4 MBq/mouse), PEG-[64Cu]CuS NPs (200 μL, 7.4 MBq/mouse), or intravenous injection of a large excess of free FA followed by FA-[64Cu]CuS NPs (200 μL, 7.4 MBq/mouse) 2 h later. uPET/CT images were acquired by an PET/CT scanner (Siemens, Knoxville, TN) 24 h after injection. For data analysis, the region-of-interest for the tumor and muscle was defined manually, and the mean signal intensities in the region-of-interest were recorded. Mice were killed by CO2 overexposure after PET/CT imaging. The tumors were removed, snap-frozen, and cut into 50-μm slices. Sections were air-dried, exposed to phosphor screen, and analyzed by an autoradiography imaging system (Fujifilm FLA-5100, Cypress, CA).
Pet ct scanner
Manufactured by Siemens
The PET/CT scanner is a medical imaging device that combines positron emission tomography (PET) and computed tomography (CT) technologies. It is used to capture detailed images of the body's internal structures and function.
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10 protocols using pet ct scanner
1
PET/CT Imaging of Tumor Targeting by Nanomaterials
2
PET Imaging of HER2-Targeted Radiotracers
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SKOV-3 (n = 2) or Ramos (n = 2) xenograft-bearing mice were injected via the tail vein with 64Cu-NODAGA-ZHER2:S1 (9.7 ± 1.4 MBq, 5 μg, 120 μL) and 64Cu-NOTA-ZHER2:S1 (10.5 ± 0.9 MBq, 5 μg, 120 μL). Additionally, SKOV-3 (n = 2) xenograft-bearing mice were injected with 68Ga-NODAGA-ZHER2:S1 (2.3 ± 0.1 MBq, 5 μg, 120 μL). Mice were anesthetized using 2.5% isoflurane/O2 and positioned on a heating pad two at a time in a PET/CT scanner (Siemens Medical Solutions, Inc.) for CT acquisition (10 min) and PET scan in list mode (20 min). Mice bearing SKOV-3 xenografts were scanned at 2, 6, and 24 h postinjection and mice with Ramos xenografts at 2 h postinjection. PET images were reconstructed using an FBP algorithm of two iterations, followed by maximum a posteriori (MAP, 18 iterations) integrative algorithms (Inveon Acquisition Workplace, version 2.0; Siemens Preclinical Solutions). Data were decay-corrected to the time of injection.
3
Quantitative SSTR PET Imaging Protocol
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The 68Ga-DOTATATE was produced following our previously published procedure (13 (link)). The study was carried out on a PET/CT scanner (Siemens Co.). Patients received an intravenous injection of 68Ga-DOTATATE (111-148 MBq). A low-dose whole-body CT scan was obtained at 40-60 min post-injection for anatomical localization and attenuation correction. PET scanning followed at 1.5 min/bed position with a 23-slice overlap. Images were reconstructed using an ordered subsets expectation-maximization algorithm and corrected for CT-based attenuation, dead time, random events, and scatter.
4
PET Imaging of [18F]DOPA Metabolism
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PET scans were carried out at the Department of Nuclear Medicine of the Radboud university medical center, using a Siemens PET/CT scanner and the radiotracer [18F]DOPA. Participants received 150 mg of carbidopa and 400 mg of entacapone 50 min before scanning, to minimize peripheral metabolism of [18F]DOPA and thereby increase central [18F]DOPA availability. The procedure started with a low dose CT scan (approximately 0.75 mCi) followed by a bolus injection of [18F]DOPA into an antecubital vein and an 89 min dynamic PET scan (approximately 5 mCi). The data were divided into 24 frames (4 × 1, 3 × 2, 3 × 3, 14 × 5 min) and reconstructed with weighted attenuation correction and time-of-flight recovery, scatter corrected, and smoothed with a 3 mm full-width-at-half-maximum (FWHM) kernel.
5
Standardized PET/CT Imaging Protocol
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Multi-Modal Imaging in Neuroendocrine Tumors
7
18F-FDG-PET Imaging Protocol for Fasting Patients
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18F-FDG-PET scan was performed on a Siemens PET-CT scanner. Patients were in a fasting state for at least 6 h prior to scanning. An intravenous line was placed in an upper extremity 15 min prior to administration of approximately 185 MBq (5mCi) of 18F-FDG, dissolved in 5 mL of saline. After waiting 30 min at rest and without visual or auditory stimuli, the PET scan was initiated with a low-dose CT scan for attenuation correction of PET data and subsequent acquisition of PET, 1 bed of 10 min duration. The analysis was visual.
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2-[18F]FDG PET/MRI and PET/CT Imaging Protocol
9
Molecular Imaging of Lung Tumor Xenografts
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6–8 weeks female BALB/c mice (with body weights of 20–25 g) were purchased from Royan Insitute (Amol, Iran). Animals were housed in wire cages under controlled conditions of temperature at 25°C, relative humidity around 50%, and 12/12 h light/dark cycles with food, and water was given ad libitum. 2 × 106 cell suspensions of human lung adenocarcinoma A549 in 100 μl PBS were injected subcutaneously in the right dorsal flank of each mouse. Palpable tumor diameter was measured using digital calipers twice weekly. When tumor size reached a mean tumor volume of 50–100 mm3, animals received a tail vein injection of 740 kBq of [68Ga]Ga-DOTA-E(cRGDfK)2 in 100 μl normal saline. After different postinjection intervals, mice were scanned on the PET/CT scanner (Siemens Medical Systems). Anaesthetized (ketamine/xylazine) animals were placed in a prone position. 20 min PET (axial FOV 148 mm) scans were carried out followed by a CT scan (spatial resolution 1.25 mm, 80 kV, and 30 mAs). Scans were reconstructed using a filtered back projection algorithm. The reconstructed PET images were fused with CT images.
10
PET Imaging of HER2-Targeted Radiotracers
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SKOV-3 (n = 2) xenograft-bearing mice were injected via the tail vein with 64Cu-CB-TE2A-G-ZHER2:342 (13.1 ± 0.9 MBq, 5 µg, 100 µL). 64Cu-CB-TE2A-GEEE-ZHER2:342 (16.6 ± 0.05 MBq, 5 µg, 100 µL) or 64Cu-NODAGA-ZHER2:S1 (12.3 ± 0.9 MBq, 5 µg, 100 µL). Mice were anesthetized using 2.5% isoflurane/O2, and positioned on a heating pad two at a time in a PET/CT scanner (Siemens Medical Solutions, Inc.) for CT acquisition (10 min) and PET scan in list mode (20 min). Mice were scanned at 2, 6, and 24 h post-injection. PET images were reconstructed using an FBP algorithm of two iterations, followed by maximum a posteriori (MAP, 18 iterations) integrative algorithms (Inveon Acquisition Workplace, version 2.0; Siemens Preclinical Solutions). Data were decay-corrected to the time of injection.
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