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B6.129 prdm1tm1clme j

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B6.129-Prdm1tm1Clme/J is a genetically modified mouse strain that harbors a targeted mutation in the Prdm1 gene. The Prdm1 gene encodes the BLIMP1 protein, which plays a critical role in the differentiation and function of various immune cell types. This mouse strain is a useful tool for studying the role of BLIMP1 in immune system development and function.

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Lab products found in correlation

B6 cg tg prdm1 eyfp 1mnz j, by Jackson ImmunoResearch (3 mentions) B6.129s fvb bcl 6tm1 1dent j bcl 6fl fl, by Jackson ImmunoResearch (2 mentions) B6.129s7 rag1tm1mom j, by Jackson ImmunoResearch (2 mentions) C57bl 6nj, by Jackson ImmunoResearch (1 mentions) B6.129s fvb bcl6tm1 1dent j, by Jackson ImmunoResearch (1 mentions) B6n 129 il21rtm1kopf j, by Jackson ImmunoResearch (1 mentions) Tg cd4 cre 1cwi bfluj, by Jackson ImmunoResearch (1 mentions) B6.129s2 ifnar1tm1agt mmjax, by Jackson ImmunoResearch (1 mentions) B6 cg sting1tm1.2camb j, by Jackson ImmunoResearch (1 mentions) B6 c cg cd79atm1 cre reth ehobj, by Jackson ImmunoResearch (1 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions)

5 protocols using b6.129 prdm1tm1clme j

1

Genetic Mouse Models for Immunology Research

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C57BL/6 (B6), B6.SJL-Ptprca Pepcb/BoyJ (B6.CD45.1), B6.129S6-Tbx21tm1Glm/J, (B6. Tbx21−/−) B6.129P2-Tcrβtm1MomTcrδtm1Mom (Tcrb−/−Tcrd−/−), B6.129S6-Sh2d1atm1Pls/J (Sh2d1a−/−), B6.129-Prdm1tm1Clme/J (Prdm1fl/fl and B6.Cg-Tg(Lck-cre)3779Nik/J, B6.129S(FVB)-Bcl-6tm1.1Dent/J (Bcl-6fl/fl, B6.129(Cg)-Foxp3tm4(YFP/icre)Ayr/J (Foxp3YFP-/Cre), B6.129S2(Cg)-Cxcr5tm1Lipp/J (Cxcr5−/−) were originally obtained from Jackson Laboratories. Il21-mCherry-Il2-emGFP dual-reporter transgenic mice were obtained from W. J. Leonard (NHLBI). Blimp-1 reporter mice were obtained from E. Meffre (Yale University). B6.129S6-Foxp3tm1DTR (Foxp3–DTR–GFP) mice were originally obtained from A Rudensky (Memorial Sloan-Kettering Cancer Center). Prdm1fl/fl mice were crossed to B6.Cg-Tg(Lck-cre)3779Nik/J mice to generate B6.Prdm1fl/fl-Lckcre/+ mice. Bcl-6fl/fl mice were crossed to Foxp3YFP-/Cre mice to generate Bcl-6fl/flFoxp3YFP/Cre mice. All mice were bred in the University of Alabama at Birmingham (UAB) animal facility. All experimental procedures involving animals were approved by the UAB Institutional Animal Care and Use Committee and were performed according to guidelines outlined by the National Research Council.
Botta D., Fuller M.J., Marquez-Lago T.T., Bachus H., Bradley J.E., Weinmann A.S., Zajac A.J., Randall T.D., Lund F.E., León B, & Ballesteros-Tato A. (2017). Dynamic regulation of T Follicular Regulatory cell responses by interleukin 2 during influenza infection. Nature immunology, 18(11), 1249-1260.
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2

Generating Genetically Engineered Mouse Models

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C57BL/6NJ (005304), B6.Cg-Tg(Prdm1-EYFP)1Mnz/J (008828), Tg(Cd4-cre)1Cwi/BfluJ (017336), B6.129-Prdm1tm1Clme/J (008100), B6.129S(FVB)-Bcl6tm1.1Dent/J (023727), B6.129S7-Rag1tm1Mom/J (002216), B6N.129-Il21rtm1Kopf/J (019115), B6;SJL-Il6ratm1.Drew/J (012944), and B6(SJL)-Il10ra tm1.1Tlg/J (028146) were purchased from Jackson Laboratories. STAT3flox were from D. Levy (New York University, New York, NY; Mouse Genome Informatics: 2384272; Lee et al., 2002 (link)). Animals were housed in specific pathogen–free enclosures at the John G. Rangos Sr. Research Center of University of Pittsburgh Medical Center Children’s Hospital of Pittsburgh. All experiments were approved by the University of Pittsburgh Animal Care Committee and the American Veterinary Medical Association. All mice were over 6 wk of age, and a mixture of male and female mice was used. All in vivo experiments were performed independently two to three times, with at least four mice per group.
He K., Hettinga A., Kale S.L., Hu S., Xie M.M., Dent A.L., Ray A, & Poholek A.C. (2020). Blimp-1 is essential for allergen-induced asthma and Th2 cell development in the lung. The Journal of Experimental Medicine, 217(7), e20190742.
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3

Genetic Mouse Models for Immunology Research

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C57BL/6 (B6), B6.SJL-Ptprca Pepcb/BoyJ (B6.CD45.1), B6.129S6-Tbx21tm1Glm/J, (B6. Tbx21−/−) B6.129P2-Tcrβtm1MomTcrδtm1Mom (Tcrb−/−Tcrd−/−), B6.129S6-Sh2d1atm1Pls/J (Sh2d1a−/−), B6.129-Prdm1tm1Clme/J (Prdm1fl/fl and B6.Cg-Tg(Lck-cre)3779Nik/J, B6.129S(FVB)-Bcl-6tm1.1Dent/J (Bcl-6fl/fl, B6.129(Cg)-Foxp3tm4(YFP/icre)Ayr/J (Foxp3YFP-/Cre), B6.129S2(Cg)-Cxcr5tm1Lipp/J (Cxcr5−/−) were originally obtained from Jackson Laboratories. Il21-mCherry-Il2-emGFP dual-reporter transgenic mice were obtained from W. J. Leonard (NHLBI). Blimp-1 reporter mice were obtained from E. Meffre (Yale University). B6.129S6-Foxp3tm1DTR (Foxp3–DTR–GFP) mice were originally obtained from A Rudensky (Memorial Sloan-Kettering Cancer Center). Prdm1fl/fl mice were crossed to B6.Cg-Tg(Lck-cre)3779Nik/J mice to generate B6.Prdm1fl/fl-Lckcre/+ mice. Bcl-6fl/fl mice were crossed to Foxp3YFP-/Cre mice to generate Bcl-6fl/flFoxp3YFP/Cre mice. All mice were bred in the University of Alabama at Birmingham (UAB) animal facility. All experimental procedures involving animals were approved by the UAB Institutional Animal Care and Use Committee and were performed according to guidelines outlined by the National Research Council.
Botta D., Fuller M.J., Marquez-Lago T.T., Bachus H., Bradley J.E., Weinmann A.S., Zajac A.J., Randall T.D., Lund F.E., León B, & Ballesteros-Tato A. (2017). Dynamic regulation of T Follicular Regulatory cell responses by interleukin 2 during influenza infection. Nature immunology, 18(11), 1249-1260.
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4

Conditional Knockout Mouse Models for Immunology Research

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C57BL/6J WT mice, B6(Cg)-Sting1tm1.2Camb/J (STING–/–) mice, B6.129S7-Rag1tm1Mom/J (Rag1–/–) mice, B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ (Cd4cre) mice, B6.129-Prdm1tm1clme/J (Prdm1fl/fl) mice, B6.Cg-Tg(Prdm1-EYFP)1Mnz/J (Blimp1-eYFP) mice, and B6.129S2-Ifnar1tm1Agt/Mmjax (IFNAR–/–) mice were purchased from the Jackson Laboratory (Bar Harbor, ME). CD4creBlimp1fl/fl mice were generated by crossing Cd4cre mice with Prdm1fl/fl mice. All the above mice were maintained in the specific pathogen-free animal facility, and GF C57BL/6 mice were bred and maintained in the GF mouse facility of the University of Texas Medical Branch. All the mouse experiments in this study were reviewed and approved by the Institutional Animal Care and Use Committee.
Yang W., Yu T., Zhou G., Yao S., Wakamiya M., Hu H., Paessler S., Sun J, & Cong Y. (2023). Intrinsic STING Switches off Pathogenetic Programs of Th1 Cells to Inhibit Colitis. Cellular and Molecular Gastroenterology and Hepatology, 15(5), 1161-1179.
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5

Comparative Study of Murine Immune Subsets

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Mouse strains C57BL/6J (Stock #: 000664), B6.Cg-Tg(Prdm1-EYFP)1Mnz/J (Stock #: 008828), B6.129-Prdm1tm1Clme/J (Stock #: 008100) and B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ (Stock #: 020505) were purchased from The Jackson Laboratory. Mice were considered young at 3-5 mo.of age. Mice were considered middle-aged at 12 mo.of age. Female and male mice were utilized for all experiments. Animal care and use were conducted according to the guidelines of the Western Michigan University Homer Stryker M.D. School of Medicine (WMed) Institutional Animal Care and Use Committee (IACUC) as well as the University of Saskatchewan (USask) University Animal Care Committee (UACC) Animal Research Ethics Board. All animals were housed and/or bred in the WMed vivarium or the USask Lab Animals Services Unit.
Pioli K.T., Lau K.H, & Pioli P.D. (2023). Thymus antibody-secreting cells possess an interferon gene signature and are preferentially expanded in young female mice. iScience, 26(3), 106223.
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