Human premixed multi analyte kit
The Human Premixed Multi-Analyte Kit is a laboratory product designed to simultaneously measure multiple analytes in human samples. The kit provides a convenient and efficient way to analyze a variety of biomolecules in a single assay.
Lab products found in correlation
51 protocols using human premixed multi analyte kit
Multiplex Biomarker Analysis of Whole Blood
Serum Biomarker Quantification Protocol
Multiplex Assay for Maternal-Fetal Chemokines
Magnetic Luminex Cytokine Quantification
Cytokine Measurement in ILC2 Cultures
Multiplex ELISA for Frozen Supernatants
Magnetic Luminex Assay Protocol
Assessing Anti-SARS-CoV-2 Antibodies and Cytokines in Breastmilk
All breastmilk samples were checked for the presence of reactive anti-S1 RBD IgG antibodies using a sandwich enzyme-linked immunosorbent assay (ELISA), as previously described in Trofin et al. (2022) [25 (link)]. We used a 1:10 dilution in the sample diluent provided by the kit. The samples were processed in accordance to the manufacturer’s indications (TestLine Clinical Diagnostics, Czech Republic, Catalog number: CoRG96-EIA COVID-19 RBD IgG, test specificity of 99.15% and test sensitivity of 99.9%). The optical densities were measured with a TECAN Infinite 200 photometer at 450 nm wavelength, and the results were processed with the Magellan software.
Ten cytokines and chemokines were simultaneously measured from the same breastmilk sample using a bead-based immunoassay with the Luminex Multiplexing Assay. We followed the manufacturer’s instructions (Human Premixed Multi-Analyte Kit, R&D systems, Minneapolis, MN, USA, Catalog number: LXSHM-10). The samples were analyzed using a Luminex 200 (USA) device. We quantified the following cytokines: TNF-α, IL-6, IFN-β, IL-10, IL-1β, IFN-γ, IL-2, GM-CSF, IL-5 and IP-10.
Multiplex Cytokine Quantification Protocol
Nasal Lavage Cytokine Analysis
Through magnetic Luminex assay, samples were analysed for interleukin‐6 (IL‐6), interleukin‐8 (IL‐8), interferon‐γ (IFN‐γ), tumor necrosis factor‐α (TNF‐α), myeloperoxidase (MPO) by a human premixed multi‐analyte kit (R&D Systems, Minneapolis, MN) and the Bio‐Plex 200 system (Bio‐Rad Lab, Hercules, CA). The analysis was performed in duplicates. To keep data quality high, coefficient of variance (CV) filtration was performed and duplicates exceeding a 20% CV‐cut off were omitted. Extrapolated values, as suggested by the machine, were used in case readouts were out of the detection range of the assay.
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