Human Ang II, tamoxifen and cell proliferation reagent 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Antibodies used to detect the protein expression levels of α-SMA (ab124964), OPN (ab8448), ERβ (ab92306), β-actin and β-tubulin were obtained from Abcam (Cambridge, MA, USA). Anti-ERα antibody was purchased from Cell Signaling Technology, Inc.(Danvers, MA, USA). ERα small interfering (si)RNA and control siRNA were purchased from Santa Cruz Biotechnology, Inc. (Dallas. TX, USA).
Human ang 2
Manufactured by Merck Group
Sourced in Germany, United States
Human Ang II is a laboratory product that functions as a peptide hormone. It is involved in the regulation of blood pressure and fluid-electrolyte balance in the human body.
Automatically generated - may contain errors
Lab products found in correlation
Human anp 1 28, by Phoenix Pharmaceuticals (2 mentions)
Human bnp 1 32, by Phoenix Pharmaceuticals (2 mentions)
Losartan, by Bio-Techne (2 mentions)
Apocynin, by Bio-Techne (2 mentions)
U73122, by Bio-Techne (2 mentions)
Pd123319, by Bio-Techne (2 mentions)
Rapamycin, by Cell Signaling Technology (2 mentions)
Ab124964, by Abcam (1 mentions)
Cell proliferation reagent, by Merck Group (1 mentions)
Ab92306, by Abcam (1 mentions)
Ab8448, by Abcam (1 mentions)
Anti erα antibody, by Cell Signaling Technology (1 mentions)
Control sirna, by Santa Cruz Biotechnology (1 mentions)
Tamoxifen, by Merck Group (1 mentions)
Male sprague dawley rat, by Charles River Laboratories (1 mentions)
Telmisartan, by Merck Group (1 mentions)
Sunitinib, by Merck Group (1 mentions)
Losartan, by Merck Group (1 mentions)
Phospho plc γ1 tyr783, by Cell Signaling Technology (1 mentions)
Plcγ1, by Cell Signaling Technology (1 mentions)
12 protocols using human ang 2
1
Ang II, Tamoxifen, and MTT Proliferation Assay
2
Peptide Therapeutics Evaluation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Throughout the in vivo and invitro study, human ANP 1–28 (catalog#005-06, Phoenix Pharmaceuticals, Burlingame, CA), human BNP 1–32 (catalog#011-03, Phoenix Pharmaceuticals, Burlingame, CA), and human ANGII (catalog#A9525, Sigma-Aldrich, St. Louis, MO) were used.
3
Angiotensin II-Induced Hypertension Model
4
Analyzing ILC2 Proliferation and Cytokine Production
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Purified ILC2s were cocultured with the indicated reagents (telmisartan; Sigma-Aldrich; PD123319, MedChemExpress) in RPMI-1640 complete medium containing 10% FBS and 1% penicillin-streptomycin, in the presence of 20 ng/ml IL-2, IL-7, and IL-33, for 3 d. Human ILC2s were purified from fresh peripheral blood, and lung and mLN ILC2s were purified from WT and AT1a−/− mice. ILC2 proliferation and cytokine production were analyzed by FACS and ELISA.
For Ang II stimulation in vitro experiments, bulk mLN lymphocytes and purified mLN and lung ILC2s were incubated in complete RPMI-1640 (supplemented with 10% MSC FBS, and 1% streptomycin and penicillin) at 37°C and in 5% CO2. Lymphocytes or ILC2s were stimulated with human Ang II (1 µM unless stated otherwise; Sigma-Aldrich) for 4 h. For cytokine protein analysis in vitro (Fig. 2, B and C ), ILC2s or lymphocytes were stimulated with 1× BFA under the indicated conditions (100 ng/ml IL-33, 1 µM Ang II, Ang II plus IL-33, or PMA plus ionomycin).
For Ang II stimulation in vitro experiments, bulk mLN lymphocytes and purified mLN and lung ILC2s were incubated in complete RPMI-1640 (supplemented with 10% MSC FBS, and 1% streptomycin and penicillin) at 37°C and in 5% CO2. Lymphocytes or ILC2s were stimulated with human Ang II (1 µM unless stated otherwise; Sigma-Aldrich) for 4 h. For cytokine protein analysis in vitro (
5
Angiotensin II Signaling in Cardiovascular Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Recombinant rat PDGF was purchased from R&D Systems. Human Ang II, losartan, sunitinib, wortmannin, and DAPT (N‐[N‐(3,5‐Difluorophenacetyl)‐L‐alanyl]‐S‐phenylglycine t‐butyl ester) were purchased from Sigma‐Aldrich. Primary antibodies of phospho‐Akt Ser473, phospho‐Akt Thr308, Akt, phospho‐PLCγ1 Tyr783, PLCγ1, 3‐phosphoinositide‐dependent protein kinase‐1 (PDK1), mammalian target of rapamycin (mTOR), and Notch2 were purchased from Cell Signaling Technology; Hey2 antibody was purchased from Beijing Biosynthesis Biotechnology; antibody of Notch1 used in Western blot was from Santa Cruz Biotechnology; and Notch1 intracellular domain (N1‐ICD) antibody used in immunohistochemistry was from Millipore.
6
Intradermal AngII Injection in Zebrafish
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human AngII (sigma) was dissolved in PBS at 10 mg/mL PBS. AngII in PBS (test) or PBS (control) was administered intradermally in adult zebrafish using Hamilton’s syringe. One day before the first injection, the fishes were anesthetized in 0.02% tricaine, weighed, and similar weight fishes were kept in a group. An amount of 1.5 µg AngII/g of zebrafish was injected intradermally at an interval of 12 h for 7, 14, and 30 days (Figure 1 A). For injection, fishes were anesthetized, immobilized dorsally into a wet foam holder (Figure 1 B), and injected intradermally. Post-injection, fishes were transferred into fresh water and revived. Revived fishes were kept in a state-of-the-art zebrafish aquarium and fed thrice per day. In the morning and evening, fishes were fed at least half an hour after the injection. After the stipulated injection period, fishes were anesthetized and weighed before sacrificing and heart isolation.
7
Peptide Hormone Assays in Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human ANP 1-28 (catalog#005-06, Phoenix Pharmaceuticals, Burlingame, CA, USA), human BNP 1-32 (catalog#011-03, Phoenix Pharmaceuticals, Burlingame, CA, USA), and human ANGII (catalog#A9525, Sigma-Aldrich, St. Louis, MO, USA) were used in all our in vivo and in vitro studies.
8
Cardiomyocyte Culture and Pharmacology
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse atrial cardiomyocyte HL-1 cells were a gift from Dr. William Claycomb at Louisiana State University Medical Center and were cultured as described previously (18 (link), 23 (link)). Nebivolol was a gift from Forest Laboratories Inc. (New York) and rapamycin was purchased from Cell Signaling Technology (Boston, MA). Human Ang II was purchased from Sigma-Aldrich. Losartan (AT1R inhibitor), PD123319 (AT2R inhibitor), apocynin (NADPH Oxidase inhibitor) and U73122 (Phospholipase C inhibitor) were purchased from TOCRIS Biosciences.
9
Hypertension Induction Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Losartan potassium (Los) was purchased from PT Kalbe Farma. The human Ang II used to induce hypertension was purchased from Sigma–Aldrich (product no. A9525, CAS no. 4474-91-3). All other chemicals used for experimental purposes were of analytical grade.
10
Cardiomyocyte Culture and Pharmacology
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!