Ache type 6 s

Manufactured by Merck Group

Sourced in United States, Spain, Germany

AChE type VI-S is a laboratory reagent manufactured by Merck Group. It is a purified acetylcholinesterase enzyme derived from electric eel. The core function of this product is to serve as a tool for the measurement and analysis of acetylcholinesterase activity in research and laboratory settings.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using ache type 6 s

Acetylcholinesterase Activity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

The acetylcholinesterase was obtained from Electrophorus electricus (AChE, Type VI-S), bovine serum albumin (BSA), physostigmine, acetylthiocholine iodide (ATCi), and Ellman's reagent 5,5'-dithiobis- (2-nitrobenzoic acid) from Sigma (St. Louis, MO, USA). The ethyl acetate, hexane, methanol, and other reagents were acquired from Vetec Química Fina, Brazil.

de Almeida e Castro V.T., Sobrinho T.J., Corrêa A.J., de Sousa Araújo T.A., Da Silva T.G, & de Amorim E.L. (2016). The Anticholinesterase Properties of Plants from the Northeast of Brazil Selected by an Ethnopharmacological Study for Disorders Relating to the Nervous System. Pharmacognosy Magazine, 12(Suppl 2), S195-S200.

+ Open protocol

+ Expand

Extraction and Characterization of C. militaris

Check if the same lab product or an alternative is used in the 5 most similar protocols

C. militaris fruiting bodies (BCRC 32219, deposited at the Bio-resource Collection and Research Centre, Food Industry Research and Development Institute, Hsinchu, Taiwan) were provided by Xin-Run Biotechnology Co. (Changhua, Taiwan). Glucose and glucosamine hydrochloride standards were purchased from Sigma-Aldrich (St Louis, MO, USA). 5,5′-dithio-bis-(2-nitrobenzoic) acid, acetylthiocholine iodide, AChE type VI-S, bovine serum albumin (BSA), and galanthamine hydrobromide were obtained from Sigma-Aldrich. All reagents and organic solvents were analytical grade. Absorbance was recorded with a UV-Vis Spectrophotometer V-530 (Jasco, Tokyo, Japan).

Tsai C.H., Yen Y.H, & Yang J.P. (2014). Finding of polysaccharide-peptide complexes in Cordyceps militaris and evaluation of its acetylcholinesterase inhibition activity. Journal of Food and Drug Analysis, 23(1), 63-70.

+ Open protocol

+ Expand

Antioxidant and Enzymatic Assays of Extracts

Check if the same lab product or an alternative is used in the 5 most similar protocols

The measurements of the activities were carried out using a 96-well microplate reader (PerkinElmer Multimode Plate Reader EnSpire) at the National Center for Biotechnology Research. The chemical products and reagents used were: Folin–Ciocalteu reagent (FCR), 1,1-diphenyl-2-picrylhydrazyl (DPPH), butylated hydroxylanisole (BHA), butylated hydroxyltoluene (BHT), quercetin, α-Tocopherol, ascorbic acid, neocuproine, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonicacid)-diammoniumsalt(ABTS), trichloroacetic acid (TCA), potassium ferricyanide (C₆N₆FeK₃), phenanthroline, silver nitrate (AgNO3), trisodium citrate (Na₃C₆H₅O₇), acetylcholinesterase from electric eel (AChE, Type-VI-S, EC 3.1.1.7, 827.84 U/mg, Sigma), butyrlcholinesterase from horse serum (BChE, EC 3.1.1.8, 7.8 U/mg, Sigma), acetylthiocholine iodide, S-Butyrylthiocholine iodide, 5,5′-Dithiobis(2-nitrobenzoic) acid (DTNB), and galantamine, which were obtained from Sigma-Aldrich; and sodium carbonate, aluminum nitrate (Al(NO₃)₃, 9H₂O), iron (III) chloride (FeCl₃), sodium bicarbonate (NaHCO₃), copper (II) chloride (CuCl₂), potassium persulfate (K₂S₂O₈), and potassium acetate (CH₃CO₂K), which were obtained from Biochem Chemopharma. All other chemicals and solvents were of analytical grade.

Srief M., Bani M., Mokrani E.H., Mennai I., Hamdi M., Boumechhour A., Abou Mustapha M., Derdour M., Kerkatou M., El-Shazly M., Bensouici C., Nieto G, & Akkal S. (2023). Evaluation of In Vitro and In Silico Anti-Alzheimer Potential of Nonpolar Extracts and Essential Oil from Mentha piperita. Foods, 12(1), 190.

+ Open protocol

+ Expand

Genipin-based Cholinesterase Inhibition Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

All chemicals were of analytical reagent grade (except those specifically indicated) and used as received. Acetonitrile (ACN) (purity 99.8 %) and formic acid (purity > 99 %) were purchased from VWR Chemicals (Barcelona, Spain). Deionized water was obtained from a Milli-Q Milipore® system A10 (Billerica, MA, USA). ChCl (98 %), Bet (>97 %), lactic acid (LaAc) (>90 %), propylene glycol (ProGly) (99 %), and propionic acid (ProAc) (99 %) were acquired from Tokyo Chemical Industry (Tokyo, Japan), Genipin analytical standard was supplied by Acros Organics (Geel, Belgium) (>98 %). Standards of lysine, AChE type VI-S was from Electrophorus electricus, whereas BChE was from equine serum, acetylthiocholine iodide (ATCI), trizma hydrochloride (Tris-HCl), fluorescein sodium salt, and a 96 well acceptor plate (Catalog n^o MATRNPS50) were obtained from Sigma-Aldrich (Madrid, Spain). The 4-(amino359 sulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F) was purchased from Tokyo Chemical Industry (Tokyo, Japan).
Unripe genipap fruits were donated by “Fazenda Lagoa” (Ponte Alta do Tocantins, TO, Brazil). The entire fruits were partially freeze-dried, grounded, and stored at −18 °C until their use.

Isabel Landim Neves M., Socas-Rodríguez B., Valdés A., Keven Silva E., Cifuentes A., Angela A. Meireles M, & Ibáñez E. (2022). Synergic effect of natural deep eutectic solvent and high-intensity ultrasound on obtaining a ready-to-use genipin extract: Crosslinking and anti-neurodegenerative properties. Food Chemistry: X, 16, 100489.

+ Open protocol

+ Expand

Bioactivity Measurements for Natural Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

The bioactivity measurements were performed on a 96-well microplate reader (Perkin Elmer Multimode Plate Reader EnSpire) at the National Center of Biotechnology Research. Gallic acid, Folin-Ciocalteu's Reagent (FCR), aluminum trichloride, 1,1'-diphenyl-2-picrylhydrazyl (DPPH), butylatedhydroxyltoluene (BHT), ascorbic acid, α-tocopherol, butylatedhydroxylanisole (BHA), dimethyl sulfoxyde (DMSO), diammonium salt (ABTS), nitro blue tetrazolium (NTB), neocuproine 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonicacid), butyrlcholinesterase from horse serum (BChE, EC 3.1.1.8, 7.8 U/mg, Sigma), acetylcholinesterase from electric eel (AChE, Type-VI-S, EC 3.1.1.7, 827.84 U/mg, Sigma), acetylthiocholine iodide, S-butyrylthiocholine iodide, galantamine, 5,5′-dithiobis (2-nitrobenzoic) acid (DTNB), anis aldehyde, sulfuric acid, and glacial acetic acid were obtained from Sigma Aldrich (Stern-heim, Germany). Potassium persulfate, copper (II) chloride and sodium carbonate were obtained from Biochem Chemopharma. All other chemicals and solvents were of analytical grade . Antibiotics were obtained from Titan Media, India.

Ouelbani R., Bensari S., Yılmaz M.A., Bensouici C., Gökalp E., Ahmed-Chaouch M., & Khelifi D. (2020). Chemical profiling and in vitro antioxidant, antibacterial and anticholinesterase activities of an endemic North African species Bellevalia mauritanica Pomel. Acta Scientifica Naturalis, 7(3), 26-45.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!