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5 μm syringe filter

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The 5-μm syringe filter is a laboratory filtration device designed to remove particulates from liquid samples. It features a 5-micron pore size membrane that effectively captures contaminants while allowing the desired liquid to pass through. This filter can be used in various research and analytical applications to ensure sample integrity and clarity.

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Lab products found in correlation

Sodium pyruvate, by Merck Group (1 mentions) Essential amino acid, by Thermo Fisher Scientific (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions) Petroff hausser chamber, by Hausser Scientific (1 mentions) Pronase from streptomyces griseus, by Merck Group (1 mentions) Influx cell sorter, by BD (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Agilent bioanalyzer, by Agilent Technologies (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Facsaria 2, by BD (1 mentions) Trizol ls, by Thermo Fisher Scientific (1 mentions) 40 μm cell strainer, by BD (1 mentions) 40 μm nylon mesh filter, by BD (1 mentions)

Spelling variants of this product

Syringe filter (154 citations) 5 μm filter (21 citations) 5 μm pore PVDF syringe filter (2 citations)

9 protocols using 5 μm syringe filter

1

Mycobacterium tuberculosis Infection Assay

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One vial of M. tuberculosis H37Rv logarithmic phase culture was thawed and centrifuged at 13,300 rpm for 10 min, and the supernatant was decanted. Subsequently, the bacterial pellet was resuspended in 1 mL of RPMI (Gibco, Carlsbad, CA, United States) supplemented with 10% fetal bovine serum, 1% non-essential amino acids (Gibco), 1% essential amino acids (Gibco), and 1% sodium pyruvate (Sigma) and then transferred to a 14 mL tube. To remove any bacterial lumps or aggregates, the suspension was passed through a 5 μm syringe filter (Millipore), and the single-cell count was obtained using a Petroff-Hausser chamber (Hausser Scientific, Horsham, PA, United States). The cells were infected at multiplicities of infection (MOIs) of 0.01, 0.1, and 1 (number of bacteria/cell).
Guerra-De-Blas P.D., Bobadilla-Del-Valle M., Sada-Ovalle I., Estrada-García I., Torres-González P., López-Saavedra A., Guzmán-Beltrán S., Ponce-de-León A, & Sifuentes-Osornio J. (2019). Simvastatin Enhances the Immune Response Against Mycobacterium tuberculosis. Frontiers in Microbiology, 10, 2097.
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2

Neuron Isolation from C. elegans

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Synchronized day 1 adult worms expressing pan-neuronal GFP were used for neuron isolation. Worms were washed with M9 buffer to remove excess bacteria. Worm pellet (~250 μl) was then washed once with 500 μl lysis buffer (200 mM DTT, 0.25% SDS, 20 mM HEPES pH 8.0, 3% sucrose), resuspended in 750 μl lysis buffer and incubated with gentle rocking for 6.5 min at room temperature. Worms were washed 6x with M9 and resuspended in 250 μl of 20 mg ml−1 pronase from Streptomyces griseus (Sigma-Aldrich). Worms were incubated at room temperature (~20 min) with periodic mechanical disruption (100x pipetting every 5 min). When most worm bodies were dissociated, ice-cold PBS buffer containing 2% fetal bovine serum (Life Technologies) was added to stop the digestion reaction. Prior to sorting, cell suspensions were passed through a 5 μm syringe filter (Millipore). Cell suspension were stained with Hoechst and Propidium Iodide for at least 30min. GFP+, Hoechst+ and Propidium Iodide- cells were sorted on a BD Influx cell sorter at the Stanford Shared FACS Facility. As a control, the corresponding GFP-, Hoechst+, and Propidium Iodide- cells were also collected. Cells were directly sorted into RTL buffer from RNAeasy kit. Sorting gates were determined by comparison to day 1 adult wildtype N2 cell suspension without any labels. At least 2000 to 10000 cells were collected per sample.
Maeder C.I., Kim J.I., Liang X., Kaganovsky K., Shen A., Li Q., Li Z., Wang S., Xu X.Z., Li J.B., Xiang Y.K., Ding J.B, & Shen K. (2018). The THO complex coordinates transcripts for synapse development and dopamine neuron survival. Cell, 174(6), 1436-1449.e20.
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3

Isolation of BAG Neurons for RNA-seq

Cited 1 time
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Embryonic cell cultures for RNA-seq were prepared from wild-type and ets-5(tm1734) mutant animals expressing the BAG cell-specific and ets-5-independent marker wzIs135[Pdel::GFP] as previously described (Christensen et al. 2002 (link); Zhang et al. 2002 (link); Fox et al. 2005 (link)). Two biological replicates were prepared for each cell type. In brief, embryos were isolated from synchronized populations of hermaphrodites that were treated with hypochlorite, and dissociated into single cells by chitinase treatment. Cells were resuspended in L-15 medium supplemented with 10% FBS (Sigma) and antibiotics and passed through a 5-μm syringe filter (Millipore). Cells were plated onto poly-D-lysine coated single-well chambered cover glasses (Lab-Tek II) and incubated overnight at 25°C.
GFP-labeled BAG neurons were isolated ∼24 h after dissociation using fluorescence-activated cell sorting (FACS). Dead cells were marked with propidium-iodide and excluded from sorted cells. Sorting was performed on a FACSAria Ilu SORP cell sorter using a 70-μm nozzle. Cells were sorted directly into RNA extraction buffer (10,000 cells/100 μL of buffer) and RNA was purified using the Arcturus PicoPure RNA isolation kit (Thermo Fisher). RNA integrity and concentration were evaluated using an Agilent Bioanalyzer.
Rossillo M, & Ringstad N. (2020). Development of specialized sensory neurons engages a nuclear receptor required for functional plasticity. Genes & Development, 34(23-24), 1666-1679.
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4

Measuring M. tuberculosis Susceptibility to INH

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M. tuberculosis H37Rv was grown to mid-log phase in supplemented 7H9. The culture was gravity-filtered through a 5-μm syringe filter (Millipore) and further diluted in the same media before plating on 7H10 containing varying concentrations of INH. Plates were incubated for 22 days at 37°C before measurement of colony size.
Sakatos A., Babunovic G.H., Chase M.R., Dills A., Leszyk J., Rosebrock T., Bryson B, & Fortune S.M. (2018). Posttranslational modification of a histone-like protein regulates phenotypic resistance to isoniazid in mycobacteria. Science Advances, 4(5), eaao1478.
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5

Cell Isolation from Transgenic Worms

Cited 2 times
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Synchronized day 1 adult transgenic worms with GFP-labeled neurons, body wall muscle, hypodermis, or intestine (rgef-1p::NuGFP, myo-3p::NuGFP, dpy-7p::NLS::GFP, and ges-1p::NuGFP) were prepared for cell isolation. Cells were isolated following the procedure described previously118 (link),119 (link) with minor modifications. Briefly, worms were subjected to SDS-DTT treatment, proteolysis, and mechanical disruption. Cell suspensions were gently passed over a 5 μm syringe filter (Millipore) for neuron cell isolation; 20 μm filter (PluriSelect) for muscle and hypodermal cell isolation. To isolate intestinal cells, cell suspensions were passed through a 40 μm cell strainer (Falcon) and then spun at 800 × g for 3 min in a tabletop centrifuge. The filtered cells were diluted in PBS/20% FBS and sorted using BD FACS Aria II (BD Biosciences). Gates for detection were set by comparison to MQD2428 (hq363[daf-2::degron::mNeonGreen]) cell suspensions prepared on the same day from a population of worms synchronized alongside the experimental samples. Positive fluorescent events were sorted directly into tubes containing TRIzol LS (Invitrogen) for subsequent RNA extraction.
Zhang Y.P., Zhang W.H., Zhang P., Li Q., Sun Y., Wang J.W., Zhang S.O., Cai T., Zhan C, & Dong M.Q. (2022). Intestine-specific removal of DAF-2 nearly doubles lifespan in Caenorhabditis elegans with little fitness cost. Nature Communications, 13, 6339.
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6

Purification of Fluorescent Neurons

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Cells were briefly subjected to SDS-DTT treatment, proteolysis, mechanical disruption, cell filtering, as described in Adult cell isolation (above). Neuron cell suspensions were passed over a 5 μm syringe filter (Millipore). The filtered cells were diluted in osmo-balanced Leibovitz’s L-15/2% FBS and sorted using a FACS Aria IIIw/ DiVa (BD Biosciences; 488 nm excitation for GFP detection, 568 nm excitation for mCherry detection). Gates for detection were set by comparison to non-fluorescent N2 cell suspensions prepared on the same day from a population of worms synchronized alongside the experimental samples. Positive fluorescent events were sorted directly into Eppendorf tubes containing Trizol LS for subsequent RNA extraction. For each sample, approximately 30,000–130,000 GFP or mCherry positive events were collected, yielding 1–10 ng total RNA.
Arey R.N., Kaletsky R, & Murphy C.T. (2019). Nervous system-wide profiling of presynaptic mRNAs reveals regulators of associative memory. Scientific Reports, 9, 20314.
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7

High-Throughput Cell Sorting Protocol

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Neuronal cell suspensions were passed through a 5μm syringe filter (Millipore), while intestinal cell suspensions (in PBS/2% FBS) were passed through a 35 μm syringe filter and a 40 μm nylon mesh filter (Falcon). Filtered cells were then diluted in the same media as above and sorted using a Sony iCyt Synergy Dual Channel high speed cell sorter (488 nm excitation). Gates were used to eliminate cells with tdTomato and autofluorescence. GFP positive fluorescent events were collected in 1.5 mL Eppendorf tubes containing 10-20 μL PBS/2% FBS, and cells were kept on ice for RNA extraction. Collected positive fluorescent events varied between 20,000 and 60,000 events for intestinal samples, and between 100,000 and 300,000 events for neuronal samples.
Özbey N.P., Imanikia S., Krueger C., Hardege I., Morud J., Sheng M., Schafer W.R., Casanueva M.O, & Taylor R.C. (2020). Tyramine Acts Downstream of Neuronal XBP-1s to Coordinate Inter-tissue UPRER Activation and Behavior in C. elegans. Developmental Cell, 55(6), 754-770.e6.
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8

Fecal Microbiome Profiling by Flow Cytometry

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Mouse stool collected from the colon was homogenized in 1.5 mL of sterile PBS. Samples were then vortexed and filtered through a 5-μm syringe filter (Millipore) to remove solid components and host cells. Filtered samples were incubated for 1 h in 37 Celsius to allow for maturation of fluorophores and then analyzed by flow cytometry.
Xia J.Y., Hepler C., Tran P., Waldeck N.J., Bass J, & Prindle A. (2023). Engineered calprotectin-sensing probiotics for IBD surveillance in humans. Proceedings of the National Academy of Sciences of the United States of America, 120(32), e2221121120.
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9

Isolating T. gondii Tachyzoites

Cited 1 time
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T. gondii RHΔku80Δhxgprt (RHΔku80) and its mutants were maintained in a confluent monolayer of human foreskin fibroblasts (HFFs) as previously described [33 (link)]. To isolate the tachyzoites, the samples were passed through a French press [34 (link)] and 5-μm syringe filter (Millipore, Darmstadt, Germany).
Xia J., Yang Y., Chen X., Song K., Ma G., Yang Y., Yao C, & Du A. (2024). An apicoplast-localized deubiquitinase contributes to the cell growth and apicoplast homeostasis of Toxoplasma gondii. Veterinary Research, 55, 10.
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