Amg9810

PEA, d₄-PEA, OEA, d₂-OEA, 2-AG, d₅-2-AG, and AMG9810 were from Cayman Chemical (Ann Arbor, MI). AEA and d₄-AEA were from R&D systems. [¹⁴C]AEA (arachidonoyl-[1-¹⁴C]ethanolamide) and rimonabant were provided by the Drug Supply Program at the National Institute on Drug Abuse. GW6471 was purchased from Sigma. All other chemicals were obtained from Fisher Scientific.

S1P (d18:1), HC-030031, AMG 9810, SEW 2871, CYM 5520, CYM 5541, JTE 013, and TY 52156 were purchased from Cayman Chemical (MI, USA). Ex 26, CYM 50260, and A 971432 were purchased from Tocris Bioscience (MN, USA). Allyl isothiocyanate (AITC) and capsaicin were purchased from MilliporeSigma (MA, USA). S1P stock (10 mM, in DMSO) was used for in vitro experiments and 10 mM S1P stock in H₂O filtered by 20 µm nylon filter (VWR International, LCC., PA, USA) was used for in vivo experiments. HC-030031 and AMG 9810 were stocked in DMSO (27.6 mM and 29.0 mM, respectively). Stock solutions were prepared as described. SEW2871 was dissolved in DMSO (50 mM). CYM 5520 and CYM 50260 were dissolved in 10 mM in DMSO. CYM5541, JTE 013, TY 52156, Ex 26, and A 971432 were prepared in 100 mM in DMSO. AITC and capsaicin were dissolved in ethanol (100 mM and 1 mM, respectively). All stocks were stored at −20°C.