The K. phaffii strain GS115 was obtained from Invitrogen (Massy, USA) and served as the host strain for protein expression. The Escherichia coli (E. coli) DH5α (Invitrogen) was utilized for the propagation of recombinant vectors. The plasmids pPIC9 and pPICZαA (Invitrogen) were employed for expressing the target proteins. The plasmids pPIC3.5K and pAO815 (Invitrogen) were utilized for expressing the chaperones.
Pao815
Manufactured by Thermo Fisher Scientific
Sourced in United States
The PAO815 is a laboratory instrument designed for the analysis of organic compounds. It utilizes a powerful analytical technique to provide accurate and reliable data for researchers and scientists. The core function of the PAO815 is to facilitate the identification and quantification of organic molecules in various sample types, supporting a wide range of applications in the scientific community.
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Lab products found in correlation
2 protocols using pao815
1
Pichia pastoris Protein Expression
2
Roseobacter Strains and Plasmids Protocol
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Three Roseobacter strains were obtained from NCIMB Ltd (Aberdeen, Scotland): R. denitrificans OCh114 (Shiba (link), 1991 (link)), O. indolifexHEL-45 (Wagner-Döbler et al. (link), 2004 (link)) and D. shibae DFL 12 (Biebl et al. (link), 2005 (link)). The E. coli strain W3110 (Bachmann (link), 1972 (link)) was sourced from historic stocks available at UCL.
BioBrick™ formatted plasmids, pSB3C5 (EU496103) and pSB1C3 (AF532313), were supplied by the Registry of Standard Biological Parts (Massachusetts, USA). Plasmids pHD1313 (Alibu et al. (link), 2005 (link)), pRPGFPSIR2rp3 (Borg , 2015 ), pUBeK (Borg , 2015 ) and pAO815 (Invitrogen, California, USA) were also used.
BioBrick™ formatted plasmids, pSB3C5 (EU496103) and pSB1C3 (AF532313), were supplied by the Registry of Standard Biological Parts (Massachusetts, USA). Plasmids pHD1313 (Alibu et al. (link), 2005 (link)), pRPGFPSIR2rp3 (Borg , 2015 ), pUBeK (Borg , 2015 ) and pAO815 (Invitrogen, California, USA) were also used.
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