293 T (ATCC, CRL-3216), HCT116 (ATCC, CCL-247EMT), PrEC LH (ATCC, PCS-440-010), LNCaP (ATCC, CRL-1740), C4-2 (ATCC, CRL-3314), PC3 (ATCC, CRL-1435), DU145 (ATCC, HTB-81), NCI-H660 (ATCC, CRL-5813), and LASCPC-01 (ATCC, CRL-3356) were originally purchased from ATCC (American Type Culture Collection) and have been confirmed by STR genotyping in our previous studies15 (link),49 (link)–51 (link). We conduct routine testing for Mycoplasma every few months. PrEC LH, LNCaP, C4-2, and PC3 were cultured in RPMI 1640. 293 T, HCT116 and DU145 was cultured in Dulbecco’s modified Eagle’s medium (Gibco, USA), supplemented with 10% fetal bovine serum (Invitrogen, USA) and 1% pen/strep (Gibco, USA). NCI-H660 and LASCPC-01 were cultured in RPMI 1640 medium, supplemented with 0.005 mg/ml Insulin, 0.01 mg/ml Transferrin, 30 nM Sodium selenite, 10 nM Hydrocortisone, 10 nM beta-estradiol, 4 mM l-glutamine (HyCloneTM, USA) and 10% FBS. Cells were maintained at 5% CO2 in a 37 °C humidified incubator.
Lascpc 01
Manufactured by American Type Culture Collection
Sourced in United States
The LASCPC-01 is a laboratory equipment product provided by American Type Culture Collection. It is a device designed for the cultivation and maintenance of cell cultures. The core function of this product is to create a controlled environment suitable for the growth and preservation of various cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Lncap, by American Type Culture Collection (4 mentions)
Du145, by American Type Culture Collection (4 mentions)
Insulin transferrin selenium, by Thermo Fisher Scientific (2 mentions)
Glutamax, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Pen strep, by Thermo Fisher Scientific (1 mentions)
Nci h660, by American Type Culture Collection (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Hct116, by Thermo Fisher Scientific (1 mentions)
Du145, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Fludarabine phosphate, by Selleck Chemicals (1 mentions)
N acetyl l cysteine, by Merck Group (1 mentions)
Nci h460, by American Type Culture Collection (1 mentions)
Mda mb 435, by American Type Culture Collection (1 mentions)
Mda pca 2b, by American Type Culture Collection (1 mentions)
Beas 2b, by American Type Culture Collection (1 mentions)
Hcc70, by American Type Culture Collection (1 mentions)
Nci h2126, by American Type Culture Collection (1 mentions)
Hs895 t, by American Type Culture Collection (1 mentions)
5 protocols using lascpc 01
1
Cell Line Characterization for Cancer Research
2
Cell Culture Protocols for Cancer Research
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LASCPC-01 (ATCC® CRL-3356) was from ATCC (Manssas, VA, USA). LASCPC-01 cells were cultured in modified HITES medium as described [45 (link)]. Briefly, HITES media containing RPMI, 5% FBS, 10 nM hydrocortisone, 10 nM beta-estradiol (Millipore Sigma, Burlington, MA, USA), insulin–transferrin–selenium, and Glutamax (Life Technologies, Carlsbad, CA, USA). HEK293T cells were cultured in DMEM (Hyclone, #SH30243.01, Logan, UT, USA) containing 10% FBS and supplemented with 1% penicillin/streptomycin. Fludarabine phosphate (#S1229, Selleckchem, Houston, TX, USA). N-acetyl-L-cysteine (A7250, Sigma), Bortizomeb (#S1013, Selleckchem). The cell line LNCaP, and LNCaP-N-MYC as well as 22Rv1, and 22Rv1-N-MYC cells were kindly provided by the Rickman Lab from Cornell Medical College. Cells were cultured in the RPMI 1640 medium (Hyclone, #SH30027.01) containing 10% Fetal Bovine Serum (FBS) (Hyclone, F1051), 1% penicillin/streptomycin (Hyclone, #SV30010) at 37 °C with 5% CO2.
3
Prostate Cancer Cell Line Acquisition and Culturing
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Human prostate cancer cell lines PC3, DU145, 22Rv1, LNCaP, and LASCPC-01 were purchased from ATCC (Manassas, VA, USA). The Doce-resistant PC3-DR and DU145-DR cell lines, generated by the long-term exposure to increasing sub-lethal concentration of Doce as previously described [37 (link)], were donated by Prof. Z. Culig, Innsbruck Medical University, Austria. All cell lines were recently authenticated and cultured according to the manufacturers’ protocols.
4
Cell Line Acquisition for Cancer Research
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E0771/Pa breast and TRAMP-C1 prostate carcinoma cells were obtained from ATCC. E0771/Bone cells were obtained from Toru Hiraga (Matsumoto Dental University, Shiojiri, Japan).12 (link) B16F10 malignant melanoma cell lines were obtained from ATCC. LLC Lewis lung carcinoma cells were obtained from Klaus Okkenhaug (University of Cambridge, Cambridge, United Kingdom), with permission from Matthew Kraman (F-star Biotechnology, Cambridge, United Kingdom). MC38 colon carcinoma cell line was obtained from Klaus Okkenhaug (University of Cambridge, Cambridge, United Kingdom), with permission from Mark Smyth (QIMR Berghofer, Brisbane, Australia). Human cell lines A375, MDA-MB-435S, A549, NCI-H1299, NCI-H460, DU145, LNCaP, PC-3, Hs895.T, SK-MEL-28, BEAS-2B, NCI-H2126, NCI-H128, HCC70, MCF-10A, MDA-PCa-2b, LASCPC-01, RWPE-1, MCF-7 and MDA-MB-231 were obtained from ATCC.
5
Cell Culture Protocol for Prostate and T-ALL Cell Lines
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PC3, LNCaP, and DU145 were purchased from the American Type Culture Collection (ATCC, Baltimore, MD, USA); PC3‐LN4 and human prostate fibroblast cell line BHPrS1 (Zemskova et al., 2015 ) were cultured in RPMI supplemented with 2 mmol·L−1 GlutaMAX (Life Technologies, Rockville, MD, USA) and 10% FBS (BioAbChem, Ladson, SC, USA) at 37 °C under 5% CO2 as reported previously. The T‐ALL cell lines HSB‐2, DU.528, KOPT‐K1, CUTLL1, HPB‐ALL, and SUP‐T1 (Padi et al., 2017 ) were cultured in RPMI 1640 supplemented with 2 mm GlutaMAX (Life Technologies) and 10% FBS (BioAbChem) at 37 °C under 5% CO2. LASCPC‐01 (ATCC) was grown in HITES media containing RPMI, 5% FBS, 10 nm hydrocortisone, 10 nm beta‐estradiol (Sigma‐aldrich, St. Louis, MO, USA), insulin–transferrin–selenium (Life Technologies), and GlutaMAX (Life Technologies). hPrEC and murine prostate epithelial tumor cells (mPrEC) cells were cultured as described previously (Song et al., 2018 ). Phoenix‐Eco cells (a gift from J. Schatz, University of Miami Health System) and fibrosarcoma cell line FLYRD18/mCAT‐IRES‐Bleo (a gift from H.G. Wendel, Memorial Sloan Kettering Cancer Center) were cultured in DMEM + 2 mm glutamine + 10% FBS. All cell lines were maintained for no more than 6 months in culture and were routinely tested for Mycoplasma.
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