Model 1025 small animal monitoring and gating system software

Mice were anesthetized with 5% isofluorane in 100% O₂ for 2 min in order to administer a nasal lavage of 4 μL of 0.75 g/mL MnCl₂ dissolved in nanopure water. Once this was administered, the animals were placed back into their cage and allowed to recover. One hour after lavage administration, the mice were exposed to 5% isoflurane and then carefully exposed to 2% isoflurane in 100% O₂ for imaging. Mice were then placed in a head holder while respiratory rate was monitored and temperature was maintained at 37 °C. This was done using an air heating system with the Model 1025 Small Animal Monitoring and Gating System Software (SA Instruments, Inc.).

Mice were anesthetized with 5% isoflurane in 100% O₂ for 2 minutes to administer a nasal lavage of 4 μL of 0.75-g/mL MnCl₂ dissolved in nanopure water. Once this was administered, the animals were placed back into their cage and allowed to recover. One hour after lavage administration, the mice were exposed to 5% isoflurane and then carefully exposed to 2% isoflurane in 100% O₂ for imaging. Mice were then placed in a head holder while respiratory rate was monitored and temperature was maintained at 37°C. This was done using an air heating system with the Model 1025 Small Animal Monitoring and Gating System Software (SA Instruments, Inc).