Inguinal dLNs were collected 7 days after immunization. Single LN cells were stained with FITC-labeled anti-CD3 (Biolegend, Cat# 100203), APC-labeled anti-CD4 (Biolegend, Cat# 100411), PE-labeled anti-PD1 (Biolegend, Cat# 135205), and PECy7-labeled anti-CXCR5 antibodies (Biolegend, Cat# 145515) to analyze Tfh cells or Alexa Fluor® 700-labeled anti-CD45 (Biolegend, Cat# 103127), FITC-labeled anti-B220 (Biolegend, Cat# 103205), APC-labeled anti-GL7 (Biolegend, Cat# 144617), and PE-labeled anti-Fas antibodies (Biolegend, Cat# 152607) to analyze GC B cells.
Pe labeled anti pd1
Manufactured by BioLegend
Sourced in United States
PE-labeled anti-PD1 is a laboratory reagent used for the detection and analysis of programmed cell death protein 1 (PD-1) expression on cells. It is a fluorescently labeled antibody that specifically binds to the PD-1 receptor, allowing for the identification and quantification of PD-1-positive cells in various experimental and clinical applications.
Automatically generated - may contain errors
Lab products found in correlation
Apc labeled anti cd4, by BioLegend (1 mentions)
Fitc labeled anti cd3, by BioLegend (1 mentions)
Fitc labeled anti mcd4, by Thermo Fisher Scientific (1 mentions)
Apc labeled anti mcd8, by Thermo Fisher Scientific (1 mentions)
Pe labeled anti ifn γ, by BioLegend (1 mentions)
Pe labeled anti foxp3 antibody, by BioLegend (1 mentions)
2 protocols using pe labeled anti pd1
1
Tfh and GC B Cell Analysis
2
T-cell Subset Analysis of Immunotherapy
Check if the same lab product or an alternative is used in the 5 most similar protocols
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To determine the therapeutic effect of the SA‐IL‐2‐modified vaccine together with PD‐1 blockade on T‐cell subsets, 100 μL blood was collected from each group on day 19 after tumor injection. To detect CD4+ and CD8+ T‐cell subsets, cells were stained with FITC‐labeled anti‐mCD4 and APC‐labeled anti‐mCD8 (eBioscience). To detect IFN‐γ+CD8+, PD‐1+CD8+ and CD4+Foxp3+ T‐cell subsets, cells were first stained with APC‐labeled anti‐mCD8 or FITC‐labeled anti‐mCD4 (Biolegend, San Diego, CA, USA), and then stained with PE‐labeled anti‐IFN‐γ, PE‐labeled anti‐PD‐1 or PE‐labeled anti‐FoxP3 antibody (Biolegend). T‐cell subsets were detected by flow cytometry.
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