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2 protocols using rabbit anti cd31 antibody

1

Immunohistochemical Analysis of Tissue Samples

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Jelly pudding was purchased from Strong Food Co., Ltd. (Shenzhen, China). Rabbit anti-CD31 antibody (Catalog number: GB11063-2), rabbit anti-CD68 antibody (Catalog number: GB113109), Cy3-labeled goat anti-rabbit antibody (Catalog number: GB21303), goat anti-rabbit secondary antibody labeled with horseradish peroxidase (HRP, Catalog number: G1213), and DAPI (Catalog number: G1012) were purchased from Servicebio Biotechnology (Wuhan, China). Chloral hydrate (Catalog number: C104202) was purchased from Aladdin Reagent Co. Ltd. (Shanghai, China). Sirius red (Catalog number: GC307014) and 3,3′-diaminobenzidine (DAB) color kits (Catalog number: G1012-1) were obtained from Servicebio Biotechnology (Wuhan, China). Suture needles and nylon sutures were purchased from Golden Circle Medical Supplies Co. (Shanghai, China).
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2

Recombinant IL-25 and Angiogenesis Pathway

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Recombinant mouse IL-25 (rmIL-25, Cat#: 587306) was brought from Biolegend, recombinant human IL-25 (rhIL-25, Cat#: 8134-IL) was purchased from R&D systems and prepared following instructions. Rabbit anti-CD31 antibody (Cat#: GB11063-2) was obtained from Servicebio. Goat anti-IL-17RB antibody (Cat#: AF1040) was obtained from R&D systems. Rabbit anti-VEGF antibody (Cat#: ab52917) and rabbit anti-β-catenin antibody (Cat#: ab32572) was from Abcam. Mouse anti-GAPDH antibody (Cat#: 60004-1-Ig) and rabbit anti-IL-17RB antibody (Cat#: 20673-1-AP) were obtained from Proteintech. Rabbit anti-phospho-Akt antibody (Cat#: 4060T), rabbit anti-Akt antibody (Cat#: 4091T), rabbit anti-phospho-ERK 1/2 antibody (Cat#: 4370S) and rabbit anti-ERK 1/2 antibody (Cat#: 4695T) were purchased from Cell Signaling Technology (CST). Glucose and streptozotocin (STZ, Cat#: S0130) were purchased from Sigma-Aldrich; and pLenti-CMV-IL-25-GFP-puro lentiviral plasmid (Lenti-IL-25-GFP, Cat#: PPL02165-4a) was obtained from Public Protein/Plasmid Library (PPL). The lentiviral packaging plasmid pMD2.G (Cat#: 12259) and psPAX2 (Cat#: 12260) were obtained from AddGene. Basement membrane matrix (Cat#: 354234) was obtained from Corning.
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