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Dmi4000 inverted

Manufactured by Leica
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Sourced in Canada, Germany

The Leica DMI4000 inverted is a research-grade microscope designed for advanced imaging applications. It features a fully motorized and automated design, allowing for precise control of various parameters. The microscope is suitable for a wide range of sample types and can be used for a variety of imaging techniques, including fluorescence, brightfield, and phase contrast microscopy.

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Lab products found in correlation

Dfc345 fx camera, by Leica (1 mentions) Lsm 510 nlo, by Zeiss (1 mentions) Ula 96 well round bottomed plates, by Corning (1 mentions)

Spelling variants of this product

DMI4000 (99 citations) DMI4000 inverted microscope (10 citations) DMI4000 B inverted fluorescent microscope (5 citations) DMI4000 automated inverted microscope (4 citations) DMI4000 B automated inverted microscope (3 citations) DMI4000 inverted fluorescent microscope (2 citations)

2 protocols using dmi4000 inverted

1

Multimodal Microscopy Imaging Protocol

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Confocal imaging was performed with Zeiss LSM 510 NLO inverted confocal microscope using a Plan Achromat 63X/1.4 Oil DIC objective (Carl Zeiss Canada Ltd., Toronto, ON, Canada). All images were acquired at a resolution of 1024 × 1024 pixels (x,y). Fluorescence microscopy was performed with a Leica DMI4000 inverted microscope (Leica Microsystems Inc, Concord, ON, Canada) using a 4×, 10× or 63× oil objective captured using a Leica DFC345 FX camera (Leica Microsystems Inc).
Ji J., Petropavlovskaia M., Khatchadourian A., Patapas J., Makhlin J., Rosenberg L, & Maysinger D. (2019). Type 2 diabetes is associated with suppression of autophagy and lipid accumulation in β‐cells. Journal of Cellular and Molecular Medicine, 23(4), 2890-2900.
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2

Tumor Spheroid Generation Protocol

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Tumor spheroids were generated by seeding 10,000 cells/well of hPheo1 cells in ultra-low attachment (ULA) 96-well round-bottomed plates (Corning, Amsterdam, The Netherlands), centrifuged for 5 min/900 g and incubated for 3 to 6 days to allow formation into a solid spheroid. Images were captured with a Leica DMI 4000 inverted fluorescence microscope (Leica Microsystems, Wetzlar, Germany).
Tabebi M., Kumar Dutta R., Skoglund C., Söderkvist P, & Gimm O. (2022). Loss of SDHB Induces a Metabolic Switch in the hPheo1 Cell Line toward Enhanced OXPHOS. International Journal of Molecular Sciences, 23(1), 560.
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