Lymphocyte subsets were assayed by using a lyse/no-wash procedure based on a single-platform technique of ten-color flow cytometry (BD FACS Canto II : U6573380-00541, USA). The reagents were BD Multitest IMK kit (catalog no.: 662965) containing BD Multitest CD3FITC/CD8PE/CD45PerCP/CD4APC and BD Multitest CD3 FITC/CD16+CD56+PE/CD45PerCP/CD19APC, and BD Multitest IMK kit lysing solution (catalog no.: 91–1087). The EDTA blood collecting tubes and trucount tubes (catalog no.: 340334) were also purchased from BD Biosciences, USA.
Multitest cd3 fitc cd16 cd56 pe cd45percp cd19apc
Manufactured by BD
Sourced in United States
The BD Multitest CD3 FITC/CD16+CD56+PE/CD45PerCP/CD19APC is a flow cytometry reagent panel. It is designed to identify and enumerate T cells, natural killer cells, and B cells in a single tube.
Automatically generated - may contain errors
Lab products found in correlation
Multitest cd3fitc cd8pe cd45percp cd4apc, by BD (3 mentions)
Multitest imk kit, by BD (2 mentions)
Edta blood collecting tubes, by BD (2 mentions)
Trucount tube, by BD (2 mentions)
Facscanto 2, by BD (2 mentions)
Multitest imk kit lysing solution, by BD (1 mentions)
Facs lysis solution, by BD (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Cellquest software, by BD (1 mentions)
3 protocols using multitest cd3 fitc cd16 cd56 pe cd45percp cd19apc
1
Flow Cytometry Analysis of Lymphocyte Subsets
2
Multiparametric Flow Cytometry Analysis
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Freshly collected whole blood was labeled at room temperature for 15 minutes with the following fluorescence‐conjugated mAbs: BD Multitest CD3 FITC/CD8 PE/CD45 PerCP/CD4 APC and BD Multitest CD3 FITC/CD16 + CD56 PE/CD45 PerCP/CD19 APC (BD Biosciences, San Jose, CA, United States). To remove red blood cells, FACS lysis solution (BD Biosciences, San Jose, CA, United States) was added and incubated at room temperature for 15 minutes. A FACSCalibur flow cytometer and CellQuest software (BD Biosciences, San Jose, CA, United States) was used to acquire and analyze the data.
3
Lymphocyte Subset Analysis by Flow Cytometry
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The detection of lymphocyte subsets were performed by using a lyse/no-wash procedure based on a single-platform technique by ten-color flow cytometry (BD FACS Canto II: U6573380-00541). The main reagents were BD Multitest IMK kit (340503) containing (BD Multitest CD3 FITC/CD8PE/CD45PerCP/CD4APC and BD Multitest CD3 FITC/CD16 + CD56PE/CD45PerCP/CD19APC), BD Multitest IMK kit lysing solution (340503); The EDTA blood collecting tubes and trucount tubes (340334) were also from BD Biosciences.
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