Liquid chromatography analysis of PJE was performed on a Waters HPLC system HPLC analysis. A Sunfire C18 ODS 4.6 × 150 mm column (Waters Corporation, Milford, MA, USA) was employed for reverse-phase separations. The mobile phases were 0.1% v/v formic acid in water and 0.1% v/v formic acid in acetonitrile at a flow rate of 1 mL/min. The elution gradient for the Sunfire C18 ODS condition was programed as an increasing percentage from 5% to 100% over 60 min, holding at 100% for 10 min, and finally reequilibrating the column at 5% for 10 min. A standard solution containing DMH1 (Tocris, Bristol, UK) was prepared by dissolving DMH1 in distilled water (5 mg/mL). The solution was filtered through a 0.45 μm membrane filter, after which HPLC was performed.
Sunfire c18 ods 4.6 150 mm column
Manufactured by Waters Corporation
Sourced in United States
The Sunfire C18 ODS 4.6 × 150 mm column is a reversed-phase high-performance liquid chromatography (HPLC) column. It features a C18 stationary phase and has dimensions of 4.6 millimeters in diameter and 150 millimeters in length.
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Lab products found in correlation
2 protocols using sunfire c18 ods 4.6 150 mm column
1
HPLC Analysis of Phytochemical Extracts
2
Quantitative HPLC Analysis of Quinic Acid
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High-performance liquid chromatography (HPLC) analysis was carried out using a Sunfire C18 ODS 4.6 × 150 mm column (Waters Corporation, USA) connected to a photodiode array detector. The mobile phase was a mixture of 0.1% (v/v) acetonitrile and water containing 0.1% (v/v) formic acid, at a flow rate of 1 mL/min. A standard solution containing quinic acid (Sigma-Aldrich, MO, USA) was prepared by dissolving these compounds in distilled water (5 mg/mL). The solution was filtered through a 0.45-µm membrane filter, after which HPLC was performed.
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