Human recombinant enzyme Hr-AChE (C1682, batch number SLBF4058) and human serum Hu-BChE (B4186, batch number SLBS4001), their substrate acetylcholine (A6625) and standard inhibitor galanthamine (G1660) were purchased from Sigma-Aldrich (St Louis, MO, USA). Hr-AChE-ICER and Hu-BChE-ICER were prepared by covalent enzyme immobilization onto fused silica capillary tubing (30 cm × 0.375 mm × 100 µm I.D.) according to a previously described procedure [22 (link)]. Resulting ICERs were connected to an on-flow LC-IT-MS/MS system (EMU FAPESP—Proc. PROEM 2014/50299-5) as biochromatography columns.
Acetylcholine a6625
Manufactured by Merck Group
Sourced in United States
Acetylcholine (A6625) is a chemical compound used in various laboratory applications. It is a neurotransmitter that plays a role in the transmission of nerve impulses. This product is commonly utilized in research and experimentation related to neurological and physiological processes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using acetylcholine a6625
1
Immobilized Cholinesterase Enzyme Reactors
2
Tissue Preparation and Compound Acquisition
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Ellagic acid (E2250), Dihydroethidium (DHE) methyl-beta-cyclodextrin (332615), acetylcholine (A6625) and D-glucose (G7021) were obtained from Sigma-Aldrich (St. Louis, MO, USA). The optimum cutting temperature compound (OCT), Tissue-Tek, was obtained from Sakura Finetek (Torrance, CA, USA).
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