To examine endothelial permeability, aortic tissues were fixed for 12 h in 2% glutaraldehyde–paraformaldehyde in 0.1 M phosphate buffer (pH 7.4), washed in 0.1 M phosphate buffer, and postfixed with 1% OsO4 dissolved in 0.1 M phosphate buffer for 2 h. Then, samples were dehydrated in ethanol and infiltrated with propylene oxide. Specimens were embedded with a Poly/Bed 812 kit (Polysciences), and 200–250-nm-thick sections were initially cut and stained with toluidine blue (T3260; Sigma-Aldrich, St. Louis, MO, USA) for light microscopy. Ultrathin sections (70 nm) were cut by a LEICA EM UC-7 (Leica Microsystems, Vienna, Austria) with a diamond knife (Diatome) and were double stained with 6% uranyl acetate (22400; Electron Microscopy Sciences, Hatfield, PA, USA) for 20 min and lead citrate (Thermo Scientific, Waltham, MA, USA) for 10 min. All sections were evaluated by transmission electron microscopy (TEM) (JEM-1011; JEOL Ltd., Tokyo, Japan) at an acceleration voltage of 80 kV.
Toluidine blue t3260
Manufactured by Merck Group
Sourced in United States
Toluidine blue (T3260) is a dye commonly used in laboratory settings. It is a metachromatic dye that exhibits different colors depending on the substance it is staining. This product is available for purchase from the Merck Group.
Automatically generated - may contain errors
Lab products found in correlation
Diamond knife, by Electron Microscopy Sciences (1 mentions)
Lead citrate, by Thermo Fisher Scientific (1 mentions)
Leica em uc7, by Leica Microsystems (1 mentions)
Poly bed 812 kit, by Polysciences (1 mentions)
Jem 1011, by JEOL (1 mentions)
Paraformaldehyde p0099, by Beyotime (1 mentions)
Mcr 501, by Anton Paar (1 mentions)
Dmp 2800, by Fujifilm (1 mentions)
4 protocols using toluidine blue t3260
1
Evaluating Endothelial Permeability via TEM
2
Toluidine Blue Staining of 3D Constructs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
3D constructs were fixed with paraformaldehyde 2% (w/v) and incubated with toluidine blue (T3260, Sigma-Aldrich, St. Louis, MO, USA) 0.05% (w/w) in water during 20 min. Then, samples were washed several times with distilled water and, finally, visualized under a stereoscopic microscope (Leica M165 C, Barcelona, Spain).
3
Mast Cell Degranulation Assay with TIIA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
As described above, mast cell line HMC-1 was collected and divided into the following four groups: (1) control group, (2) C48/80 group, (3) C48/80 + 5 μmol/L TIIA (TIIA-5) group and (4) C48/80 + 10 μmol/L TIIA (TIIA-10) group. Cells in different groups were treated with 0.5 μg/mL C48/80 and different concentrations of TIIA. After incubation, the cells were fixed with 4% paraformaldehyde (P0099, Beyotime, Shanghai, China) for 25 min, and then stained with toluidine blue (T3260, Sigma-Aldrich, St. Louis, MO) for 1–2 min. Finally, mast cell degranulation was observed under an optical microscope.
4
Formulation and Characterization of Pre-OHGel Ink
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To prepare the pre-OHGel ink, 4 wt% glycerol was mixed with deionized water, then 8 wt% P(SPMA0.5-r-MMA0.5) was dissolved in the binary solvent with 70 °C water bath and magnetic stirring for 1 h. Finally, 0.02 wt% polyether-modified siloxane surfactant (BYK-348, BYK Additives & Instruments) was added into the solution at room temperature as surface tension modifier. The as-prepared ink was filtered through polytetrafluoroethylene (PTFE) syringe filters (0.45 µm pore size; C2008S1, Ossila) to remove impurities. Trace amounts of toluidine blue (T3260, Sigma-Aldrich) could be dissolved in the pre-OHGel ink to enhance the visibility of printed OHGel. The apparent viscosity of the inks with different polyelectrolyte load was examined under ambient condition using a rheometer (MCR 501, Anton-Paar). Surface tension of the pre-OHGel inks with or without BYK-348 surfactant was measured by a micro-tensiometer (Ez-Pi plus, Kibron). DOD inkjet printing was performed by a Dimatix Materials Printer (DMP-2800, Fujifilm).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!