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Anti baff

Manufactured by R&D Systems
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Anti-BAFF is a recombinant human protein that neutralizes the activity of B cell activating factor (BAFF), also known as BLyS. BAFF is a cytokine that plays a crucial role in the survival and differentiation of B cells. Anti-BAFF binds to and neutralizes BAFF, thereby modulating B cell function.

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Lab products found in correlation

Anti active caspase 3, by BD (1 mentions) Anti cleaved caspase 8, by Cell Signaling Technology (1 mentions) Anti ly6g, by Thermo Fisher Scientific (1 mentions) Anti ly6c, by Thermo Fisher Scientific (1 mentions) Anti pd l1, by Thermo Fisher Scientific (1 mentions) Axiocam 503, by Zeiss (1 mentions) Anti gfp, by Thermo Fisher Scientific (1 mentions) M5 114, by BioLegend (1 mentions) Anti cd8 rm2206, by BioLegend (1 mentions) Anti cd4 rm4 5, by BioLegend (1 mentions) Su6656, by Merck Group (1 mentions) Cytochalasin d, by Merck Group (1 mentions) Ly294002, by Merck Group (1 mentions) Cytofix cytoperm solution, by BD (1 mentions) Perm wash buffer, by BD (1 mentions)

4 protocols using anti baff

1

Multiparametric Tissue Analysis Protocol

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Histologic analysis was performed on snap frozen tissues that were fixed in acetone, blocked with 10% FCS, and stained with anti-active caspase-3 (BD Biosciences), anti-cleaved caspase-8 (Cell Signaling Technology), anti-PD-L1, anti-LY6C, anti-LY6G (all eBioscience), and anti-BAFF (R&D) antibodies. Fluorescent images were taken with an Axiocam 503 color microscope (ZEISS) and quantified using ImageJ. Conventional histology images were taken using the Brightfield microscope.
Liu W., Stachura P., Xu H.C., Váraljai R., Shinde P., Ganesh N.U., Mack M., Van Lierop A., Huang A., Sundaram B., Lang K.S., Picard D., Fischer U., Remke M., Homey B., Roesch A., Häussinger D., Lang P.A., Borkhardt A, & Pandyra A.A. (2021). BAFF Attenuates Immunosuppressive Monocytes in the Melanoma Tumor Microenvironment. Cancer Research, 82(2), 264-277.
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2

Multicolor Flow Cytometry Phenotyping

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The following antibodies were used: anti-CD45 (30-F11), anti-CD31 (390), anti-PDPN (8.1.1), anti-MadCAM (MECA-367), anti-B220 (RA3-6B2), antibody to α-smooth muscle actin (1A4), anti-Thy-1 (53-2.1), anti-CD140α (APA5), anti-CD140b (APB5), anti-CD106 (429), anti-CD11b (M1/70), anti-CD11c (N418), anti-Gr1 (RB6-8C5), antibody to major histocompatibility complex class II (M5/114.15.2), anti-CD4 (RM4.5) and anti-CD8 (RM2206; all from Biolegend); anti-BAFF (121808 from R&D Systems); and anti-GFP (A10263 from Life Technologies).
Cremasco V., Woodruff M.C., Onder L., Cupovic J., Nieves-Bonilla J.M., Schildberg F.A., Chang J., Cremasco F., Harvey C.J., Wucherpfennig K., Ludewig B., Carroll M.C, & Turley S.J. (2014). B cell homeostasis and follicle confines are governed by fibroblastic reticular cells. Nature Immunology, 15(10), 973-981.
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3

Cellular Adhesion Pathway Inhibition

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Cytochalasin D, SU6656 and LY-294002 (Sigma-Aldrich, St. Louis, MO, USA) were used at 10 μM, 1 μM and 1 μM, respectively. Blocking antibodies anti-CD2 (clone TS2/18), anti-CD31 (clone HEC7), anti-ICAM-1 (clone W-CAM-1), anti-LFA-3 (clone TS2/9) and their relevant isotype controls were used at 10 μg/ml (Thermofisher, Villebon sur Yvette, France). Blocking antibodies anti-CXCL12 (Selleckchem, Houston, USA), anti-BAFF and anti-APRIL (clone 670820, R&D Systems, Minneapolis, USA) were used at 4 μM, 20 ng/ml and 1 μg/ml respectively.
Boissard F., Tosolini M., Ligat L., Quillet-Mary A., Lopez F., Fournié J.J., Ysebaert L, & Poupot M. (2016). Nurse-like cells promote CLL survival through LFA-3/CD2 interactions. Oncotarget, 8(32), 52225-52236.
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4

Quantifying Intracellular BAFF Expression

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Cells were stained with anti-BAFF (clone 121808, R&D Systems, FITC-coupled). For intracellular staining cells were fixed with Cytofix/Cytoperm solution (BD) for 20 min on ice and washed twice with PermWash buffer (BD). Then cells were stained for 30 min at RT with anti-BAFF and then for 20 min at RT with a goat anti-rat IgG-BV421 antibody (Biolegend).
Zehentmeier S., Lim V.Y., Ma Y., Fossati J., Ito T., Jiang Y., Tumanov A.V., Lee H.J., Dillinger L., Kim J., Csomos K., Walter J.E., Choi J, & Pereira J.P. (2022). Dysregulated stem cell niches and altered lymphocyte recirculation cause B and T cell lymphopenia in WHIM syndrome. Science immunology, 7(75), eabo3170.
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