Human EOC cell lines, CAOV3 and OVCAR3 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). EOC cells were maintained in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen), 100 U/mL penicillin and 100 µg/mL streptomycin (Sinopharm) in a in humidified atmosphere at 37°C with 5% CO2.
Penicillin
Manufactured by Sinopharm
Sourced in United States
Penicillin is a type of laboratory equipment used in the production and testing of antibiotics. It functions as a fermentation vessel, providing a controlled environment for the growth and cultivation of microorganisms involved in the production of penicillin and other antibiotic substances.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by Sinopharm (3 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Ovcar3, by American Type Culture Collection (1 mentions)
Caov3, by American Type Culture Collection (1 mentions)
Filter paper, by Cytiva (1 mentions)
Kanamycin, by Sinopharm (1 mentions)
Ampicillin, by Sinopharm (1 mentions)
Monopotassium phosphate kh2po4, by Sinopharm (1 mentions)
Magnesium chloride mgcl2, by Sinopharm (1 mentions)
Dipotassium phosphate k2hpo4, by Sinopharm (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Tween 20, by Solarbio (1 mentions)
Sodium chloride (nacl), by Sinopharm (1 mentions)
Phosphorus pentoxide, by Merck Group (1 mentions)
Concentrated sulfuric acid, by Merck Group (1 mentions)
Tris hcl, by Merck Group (1 mentions)
Pepstatin a, by Merck Group (1 mentions)
Rnase, by Merck Group (1 mentions)
Pbs buffer, by Merck Group (1 mentions)
Graphite powder, by Merck Group (1 mentions)
5 protocols using penicillin
1
Culturing Ovarian Cancer Cell Lines
2
Rapid Immunoassay for Tetracycline Detection
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Trimethoxyoctadecylsilane (C21H46O3Si), bovine serum albumin (BSA) and tetracycline hydrochloride (C22H24N2O8·HCl) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ampicillin, penicillin, streptomycin, kanamycin, dipotassium phosphate (K2HPO4), monopotassium phosphate (KH2PO4), sodium chloride (NaCl) and magnesium chloride (MgCl2) were purchased from Sinopharm (Hong Kong, China). Tween-20 was purchased from Solarbio (Beijing, China). The filter paper was purchased from Whatman (Maidstone, UK). Tetracycline-BSA antigen and anti-tetracycline monoclonal antibody were purchased from Suzhou Kuaijiekang Co., Ltd. (Suzhou, China). Commercial tetracycline ELISA kit was purchased from Shenzhen Finder Biotech Co., Ltd. (Shenzhen, China). The heating plate (YH-946B) was purchased from Shanghai Yuxing Electronics Co., Ltd. (Shanghai, China). The inkjet printer (IP2780) was purchased from Canon (Tokyo, Japan).
3
Graphene Oxide Synthesis Protocol
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95% ethanol (Shenyang Chemical Reagent Plant), barium chloride, sodium nitrate (NaNO3), hydrogen peroxide, concentrated sulfuric acid (H2SO4), graphite powder, potassium permanganate (KMnO4), potassium peroxydisulfate (K2S2O8), phosphorus pentoxide (P2O5), Tris-HCl, Trit onX-100, PBS buffer, protease inhibitor (Aprotinin, Leupeptin, Pepstatin A), Dnase, Rnase and chloral hydrate dry powder were all purchased from Sigma Company, the penicillin and gentamycin were purchased from Sinopharm Group.
4
Eriocitrin Protects HK-2 Cells from OGD/R
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The human renal tubular epithelial cell line HK-2 was purchased from the cell bank of the Chinese Academy of Sciences. HK-2 cells were stored in Dulbecco’s modified eagle medium (DEME) medium (Invitrogen, Carlsbad, CA, United States) supplemented with L-glutamine (Gibco, New York, CA, USA), 100 U/mL penicillin and 100 mg/mL streptomycin (Sinopharm Chemical Reagent, Beijing, China). Then, the cells were incubated in an incubator with 5% CO2 at 37 °C.
Cell grouping and treatment: Control group: no treatment was given. OGD/R group: treated according to the OGD/R model establishment method. Eriocitrin groups: before OGD/R treatment, HK-2 cells were treated with different doses of eriocitrin (1, 2, or 4 µM/mL) for 30 minutes, and eriocitrin was continuously administered at the same dose as the initial until the end of the experiment. In addition, the DUSP14-specific inhibitor, PTP inhibitor IV (50 µM, San Diego, CA, United States), treatment started at 30 minutes before OGD/R treatment and ended at the end of the experiment.
Cell grouping and treatment: Control group: no treatment was given. OGD/R group: treated according to the OGD/R model establishment method. Eriocitrin groups: before OGD/R treatment, HK-2 cells were treated with different doses of eriocitrin (1, 2, or 4 µM/mL) for 30 minutes, and eriocitrin was continuously administered at the same dose as the initial until the end of the experiment. In addition, the DUSP14-specific inhibitor, PTP inhibitor IV (50 µM, San Diego, CA, United States), treatment started at 30 minutes before OGD/R treatment and ended at the end of the experiment.
5
Hypertension Rat Brain Transcriptome Analysis
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Eight crude herbs of LXTYF were purchased from Tongrentang Chinese Medicine Company (Since 1669). Spontaneous hypertension rats (SHRs) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd., and animal license number was SCXK (Beijing) 2016-0006. Collagenase type VII was purchased from Sigma-Aldrich (St Louis, MO, USA). Brain stereotaxic instruments and electric drills were bought from Yuyan Instruments Co., Ltd. (Shanghai, China). TJ-1A microinjection pump was purchased from Lange Constant Flow Pump Co., Ltd. (Baoding, China). Microneedle was purchased from Gaoge Industry & Trade Co., Ltd. (Shanghai, China). Chloral hydrate and penicillin were bought from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). TRIzol® Reagent and TBS380 Picogreen were purchased from Invitrogen (Carlsbad, CA, USA). Magnetic frame Ribo-Zero Magnetic kit was bought from EpiCentre (USA). TruSeqTM Stranded Total RNA Library Prep Kit, UNG enzyme, HiSeq 4000 PE Cluster Kit, and HiSeq 4000 SBS Kit (300cycles) were purchased from Illumina (San Diego, CA, USA). Certified Low Range Ultra Agarose was purchased from Bio-Rad (Hercules, CA, USA).
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