Anti phospho eif4e

Manufactured by Cell Signaling Technology

Anti-phospho-eIF4E is a primary antibody product developed by Cell Signaling Technology. It is designed to detect phosphorylated eIF4E, a key protein involved in the regulation of mRNA translation.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

EIF4E (87 citations) Anti-eIF4E (36 citations) Phospho-eIF4E (7 citations)

5 protocols using anti phospho eif4e

Comprehensive Immunoblot Analysis of Inflammatory Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies (Abs) and reagents were used in present studies: anti-IL-1β (human specific, mouse specific, and human & mouse specific), anti-phospho NF-κB, anti-NF-κB, anti-phospho ERK, anti-ERK, anti-phospho p38, anti-p38, anti-phospho 4E-BP1, anti-4E-BP1, anti-phospho Mnk1, anti-Mnk1, anti-phospho eIF4E, anti-eIF4E, anti-phospho MK2, anti-MK2, and anti-streptavidin-HRP antibodies were purchased from cell signaling technology (Danvers, MA). Anti-β-actin, Dimethyl sulfoxide, Actinomycin D, Cyclohexamide, ultrapure E. coli O111:B4 LPS, Rapamycin, PD98059, and SB202190 were purchased from Sigma-Aldrich (St. Louis, MO). Anti-NLRP3 antibody was purchased from Adipogen (San Diego, CA). Torin 1 and FR180204 were purchased from Merck Millipore (Billerica, MA). CGP57380 and MK25 were purchased from TOCRIS (Ellisville, MO, USA) and Cayman Chemical (Ann Arbor, MI), respectively. Recombinant human M-CSF and mouse M-CSF were purchased from R&D system (Minneapolis, MN).

Chung Y.H., Kim D.H, & Lee W.W. (2016). Monosodium urate crystal-induced pro-interleukin-1β production is post-transcriptionally regulated via the p38 signaling pathway in human monocytes. Scientific Reports, 6, 34533.

+ Open protocol

+ Expand

Western Blot Antibody Optimization

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following primary Abs were used: rabbit polyclonal anti-phospho-Jak2 (Tyr^1007/1008), anti-phospho-Stat3 (Tyr⁷⁰⁵), anti-phospho-Stat5 (Tyr⁶⁹⁴), anti-phospho-Akt (Ser⁴⁷³), anti-phospho-eIF4E (Ser²⁰⁹), anti-phosp ho-4E-BP1 (Ser⁶⁵) used at 1/1000 dilution (Cell Signaling Technology), and mouse monoclonal anti-β-actin (1/5000; Sigma-Aldrich; St. Louis, MO) and anti-GAPDH (1/5000; Abcam, Cambridge, MA) antibodies. Goat anti-mouse EPOR (use at 0.2ug/ml) was purchased from R&D, and anti-total 4E-BP1, anti-total Jak2, anti-total eIF4E, and anti-total Akt were from cell Signaling. Secondary Abs included: anti-goat IgG-HRP (1/2500; Santa Cruz, Santa Cruz, CA), anti-rabbit and anti-mouse IgG-HRP (1/2000; Cell Signaling Technology).

Julius A., Desai A, & Yung R.L. (2017). Recombinant human erythropoietin stimulates melanoma tumor growth through activation of initiation factor eIF4E. Oncotarget, 8(18), 30317-30327.

+ Open protocol

+ Expand

Western Blot Analysis of Protein Signaling

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proteins were obtained from whole cell lysates using lysis buffer (20 mM Tris–HCl [pH 7.5], 1% NP-40, 10% glycerol, and 150 mM NaCl supplemented with 1% protease and phosphatase inhibitor mixtures) (Sigma) as previously described [28 (link)]. The primary Abs used were: mouse anti-Akt1/2, rabbit anti-phospho-Ser473Akt, rabbit anti-Erk1/2 or anti-phospho-Erk1/2, anti-Mnk1, anti-4E-BP1, anti-AIF, anti-PHB1, anti-PHB2, anti-phospho(Ser/Thr)-Akt substrate, anti-eIF4E, anti-Phospho-eIF4E, anti-C-Raf and anti-phospho-C-Raf (Ser289/296/301) (Cell signaling Technology), mouse anti-PARP, rabbit anti-Bcl-2 and anti-c-Myc (Santa Cruz Biotechnology), and anti-βactin (Sigma). Horseradish peroxidase (HRP)–conjugated secondary antibodies (1:1000) were from DakoCytomation. Blotted proteins were detected and quantified using the Immobilon Western Chemiluminescent HRP Substrate (Millipore) and a bioimaging system (Genesnap; Syngene).

Bentayeb H., Aitamer M., Petit B., Dubanet L., Elderwish S., Désaubry L., de Gramont A., Raymond E., Olivrie A., Abraham J., Jauberteau M.O, & Troutaud D. (2019). Prohibitin (PHB) expression is associated with aggressiveness in DLBCL and flavagline-mediated inhibition of cytoplasmic PHB functions induces anti-tumor effects. Journal of Experimental & Clinical Cancer Research : CR, 38, 450.

+ Open protocol

+ Expand

Antibodies and Reagents for SLFN11 Study

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used in our study: anti-SLFN11 (Atlas antibodies, HPA023030), anti-GAPDH (Sigma-Aldrich), anti-RPS4X (Abcam), anti-phospho-RPS6 or anti-phospho-S6 (Ser235/236; Cell Signaling Technology), anti-RPS6 (or S6; Cell Signaling Technology), anti-phospho-eIF4E (Ser209; Cell Signaling Technology), anti-eIF4E (Cell Signaling Technology), anti-Bax (Cell Signaling Technology), anti-Bak (Cell Signaling Technology), anti-Bcl-2 (Cell Signaling Technology), anti-Ki-67 (Abcam), anti-Flag (Cell Signaling Technology), Normal rabbit IgG (Cell Signaling Technology), Alexa Fluor 488 goat anti-mouse IgG (Invitrogen), Alexa Fluor 594 goat anti-rabbit IgG (Invitrogen), anti-SLFN11 (Santa Cruz, sc-374339), and BrdU Cell Proliferation Assay Kit (Cell Signaling Technology). Cisplatin was purchased from MedChem Express. INK128 (also known as sapanisertib, MLN0128, and TAK-228) was purchased from SelleckChem.

Zhou C., Liu C., Liu W., Chen W., Yin Y., Li C.W., Hsu J.L., Sun J., Zhou Q., Li H., Hu B., Fu P., Atyah M., Ma Q., Xu Y., Dong Q., Hung M.C, & Ren N. (2020). SLFN11 inhibits hepatocellular carcinoma tumorigenesis and metastasis by targeting RPS4X via mTOR pathway. Theranostics, 10(10), 4627-4643.

+ Open protocol

+ Expand

Western Blotting Antibody Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dimethyl sulfoxide was from Thermo Fisher Scientific (#BP231). Antibodies for western blotting were purchased from Sigma: rabbit anti-mGluR7a (#07-239, 1:2000 dilution) and mouse anti-puromycin (#MABE343, 1:1000 dilution); from Synaptic Systems: rabbit anti-mGluR7b (#191 203, 1:2000 dilution); from Proteintech: anti-GAPDH (#60004-1, 1:2500); from Thermo Fisher Scientific: rabbit anti-phospho-eIF2α (#MA5-15133, 1:1000 dilution); from Bioss: rabbit anti-PCDH7 (#bs-11085R, 1:1000 dilution); from Abcam: anti-phospho-eIF4E (#2069, 1:1000 dilution); from Cell Signaling Technology: rabbit anti-FMRP (#7104, 1:2500 dilution); rabbit anti-N-cadherin (#13116, 1:2500 dilution); rabbit anti-ERK1/2 (#4695, 1:5000 dilution); rabbit anti-phospho-ERK1/2 (#4370, 1:5000 dilution); rabbit anti-mTOR (#2972, 1:2500 dilution), rabbit anti-phospho-mTOR (#2971, 1:2500 dilution); rabbit anti-eIF4E (#2067, 1:2500 dilution); rabbit anti-eIF2α (#5324, 1:2500 dilution); rabbit anti-eIF4G (#2498, 1:2500 dilution). Secondary antibodies were from Cell Signaling Technology: anti-mouse HRP (#7076, 1:2500 dilution) and from Jackson ImmunoResearch: anti-rabbit HRP (#711‐035-152, 1:2500 dilution).

Kumar V., Lee K.Y., Acharya A., Babik M.S., Christian-Hinman C.A., Rhodes J.S, & Tsai N.P. (2024). mGluR7 allosteric modulator AMN082 corrects protein synthesis and pathological phenotypes in FXS. EMBO Molecular Medicine, 16(3), 506-522.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!