Log In Sign up
The largest database of trusted experimental protocols

F3165

Manufactured by OriGene
Visit Supplier

F3165 is a lab equipment product from OriGene. It is a multi-purpose device designed for various laboratory applications. The core function of F3165 is to facilitate precise and reliable measurements or analyses in a controlled laboratory setting.

Automatically generated - may contain errors

Lab products found in correlation

Nitrocellulose membrane, by Thermo Fisher Scientific (2 mentions) Bolt gel, by Thermo Fisher Scientific (2 mentions) Gtu 88, by Merck Group (2 mentions) Ab219649, by Abcam (2 mentions) Ta180093, by OriGene (2 mentions) Jbw301 05 636, by Merck Group (2 mentions) Hpa040067, by Abcam (2 mentions) Dnase 1, by Merck Group (1 mentions) Human ifn γ, by Thermo Fisher Scientific (1 mentions) Collagenase type 2, by Worthington (1 mentions) Hek293 cell, by American Type Culture Collection (1 mentions) Dual specific luciferase assay kit, by Promega (1 mentions) Polybrene, by Merck Group (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Sybr green, by Bio-Rad (1 mentions) Trizol, by Takara Bio (1 mentions) Murine m csf, by Thermo Fisher Scientific (1 mentions) H6908, by Merck Group (1 mentions) Ab93159, by Abcam (1 mentions) Ab109272, by Abcam (1 mentions)

3 protocols using f3165

1

Antibody Characterization and Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse monoclonal antibodies against Flag (Origene, 1:2000, F3165), HA (Origene, 1:2000, H6908), β-actin (Sigma, 1:10,000, A2228), Myc (CST, 1:1000, 5605), p-IκBα (CST, 1:1000, 9246 L); rabbit antibodies against p-IRF3 (CST, 1:500, 37829), USP19 (Abcam, 1:1000, ab93159), TRIF (Abcam, 1:1000, ab180689), p65 (Santa Cruz Biotechnology, 1:1000, 71675), p-p65(S536) (CST, 1:1000, 3033),TBK1 (Abcam, 1:1000, ab40676) and p-TBK1 (Abcam, 1:1000, ab109272), ubiquitin (Abcam, 1:500, ab134953), K27-linkage specific polyubiquitin (Abcam, 1:1000, 181537), TLR3 (CST, 1:500, 6961), TLR4 (R&D, 1:500, AF1478), TRAM (Abcam, 1:500, ab96106), KCTD10 (Proteintech, 1:1000, 27279–1-AP); poly(I:C) (Invivogen), LPS (Sigma), R848 (Invivogen), PGN (Invivogen), human IFN-γ (Peprotech), murine M-CSF (Peprotech), Trizol (Takara Bio), SYBR Green (BIO-RAD), dual-specific luciferase assay kit (Promega, E1980), polybrene (Millipore,TR-1003-G), type II collagenase (Worthington), DNase I (Sigma-Aldrich), and d-galactosamine hydrochloride (D-Gal) (Sigma); and ELISA kits for TNF (Biolegend), IL-6 (Biolegend), CXCL10 (Boster) and IFN-β (PBL) were purchased from the indicated companies. HEK293 cells were obtained from ATCC. SeV (Cantell strain) (Charles River Laboratories), HSV-1 (KOS strain) (China Center for Type Culture Collection, Wuhan, China) were obtained from the indicated companies.
Wu X., Lei C., Xia T., Zhong X., Yang Q, & Shu H.B. (2019). Regulation of TRIF-mediated innate immune response by K27-linked polyubiquitination and deubiquitination. Nature Communications, 10, 4115.
+ Open protocol
+ Expand
2

Whole-Cell Extraction Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole-cell extracts (WCE) were prepared by lysing cells in two volumes of Lysis buffer (50 mmol/L Tris pH 7.4, 150 mmol/L NaCl, 5 mmol/L EDTA, 50 mmol/L HEPES, 1% v/v Triton-X100, 0.1% v/v SDS). Thirty to 60 μg of WCE was resolved on 4%–12% Bolt Gels (Thermo Fisher Scientific). Protein was transferred to nitrocellulose membrane (Thermo Fisher Scientific) and blotted for SF3B1 (Bethyl Laboratories: A300–996A; RRID:AB_805834)), Flag M2 (Sigma-Aldrich: F3165; RRID:AB_439685), mRFP (Origene:TA180093; RRID:AB_2622287) and γ-tubulin (GTU-88:Sigma Aldrich; RRID:AB_532292), ATM (2C1:Abcam; RRID:AB_368161), BRCA2 (OP95:Millipore; RRID: AB_2067762), ATR (N19:SCBT; RRID:AB_630893), Mre11 (4895:Cell Signaling Technology; RRID:AB_2145100), BRCA1 (D-9:SCBT; RRID:AB_626761), RAD51 (SCBT:3C10/sc-53428; RRID:AB_630180), γH2AX (Millipore:JBW301/05–636; RRID:AB_309864), GAPDH (Sigma: HPA040067; RRID:AB_10965903), vinculin (Abcam:ab219649; RRID: AB_2819348), β-actin (SCBT:C4; RRID: AB_2714189), and GFP (D5.1:Cell Signaling Technology; RRID:AB_1196615).
Lappin K.M., Barros E.M., Jhujh S.S., Irwin G.W., McMillan H., Liberante F.G., Latimer C., La Bonte M.J., Mills K.I., Harkin D.P., Stewart G.S, & Savage K.I. (2022). Cancer-Associated SF3B1 Mutations Confer a BRCA-Like Cellular Phenotype and Synthetic Lethality to PARP Inhibitors. Cancer Research, 82(5), 819-830.
+ Open protocol
+ Expand
3

Whole Cell Proteome Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole cell extracts (WCE) were prepared by lysing cells in two volumes of Lysis Buffer (50mM TRIS pH 7.4, 150 mM NaCl, 5 mM EDTA, 50 mM HEPES, 1% v/v Triton-X100, 0.1% v/v SDS). 30-60µg of WCE was resolved on 4-12% Bolt Gels (ThermoFisher). Protein was transferred to nitrocellulose membrane (ThermoFisher) and blotted for SF3B1 (Bethyl Laboratories:A300-996A; RRID:AB_805834)), Flag M2 (Sigma-Aldrich: F3165; RRID:AB_439685), mRFP (Origene:TA180093; RRID:AB_2622287) and γ-tubulin (GTU-88:Sigma Aldrich; RRID:AB_532292), ATM (2C1:Abcam; RRID:AB_368161), BRCA2 (OP95:Millipore; RRID: AB_2067762), ATR (N19:SCBT; RRID:AB_630893), Mre11 (4895:CellSignallingTechnology; RRID:AB_2145100) BRCA1 (D-9:SCBT; RRID:AB_626761), RAD51 (SCBT:3C10/sc-53428; RRID:AB_630180), γ-H2AX (Millipore:JBW301/05-636; RRID:AB_309864), GAPDH (Sigma: HPA040067; RRID:AB_10965903), Vinculin (Abcam:ab219649; RRID: AB_2819348), β-Actin (SCBT:C4; RRID: AB_2714189) and GFP (D5.1:CellSignalingTechnology; RRID:AB_1196615).
Lappin K.M., Barros E.M., Jhujh S.S., Irwin G.W., McMillan H., Liberante F.G., Latimer C., La Bonte M.J., Mills K.I., Harkin D.P., Stewart G.S, & Savage K.I. (2022). Cancer-Associated SF3B1 Mutations Confer a BRCA-Like Cellular Phenotype and Synthetic Lethality to PARP Inhibitors. Cancer Research, 82(5), 819-830.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!