All mouse experiments were approved by the animal care committee of Korea University and carried out in accordance with ethical standards of government and institutional guidelines and regulations. To establish subcutaneous xenograft models, 2 × 106 cells of each cell line in PBS were mixed with Matrigel (Invitrogen) at a ratio of 50% and then subcutaneously transplanted into 5–6-week-old BALB/c nu/nu mice (Orient Bio, Inc., Seongnam, Korea). Tumor sizes were calculated using the following formula: tumor volume (mm3) = longest diameter of tumor (mm) × shortest diameter of tumor (mm) 2/2. The mice were sacrificed if unexpectedly found to be moribund or they showed rapid body weight loss (over 20% of body weight), hunched posture, lethargy or persistent recumbency, or a tumor estimated to be more than 10% of the body weight. Mice with tumors exceeding 1000 mm3 were sacrificed. Mice without tumors were maintained for up to 6 months, and then sacrificed by carbon dioxide asphyxiation in accordance with government and institutional guidelines and regulations.
Balb c nu nu mice
Manufactured by Orient Bio
Sourced in Cameroon
BALB/c nu/nu mice are a strain of athymic nude mice. They are characterized by a genetic mutation that results in the absence of a thymus gland and, consequently, a deficiency in T-cell-mediated immunity. These mice are commonly used in biomedical research as a model for studying immune system function, cancer, and the effects of immunotherapies.
Automatically generated - may contain errors
Lab products found in correlation
Matrigel, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Caki 1, by Welgene (1 mentions)
1640 medium, by Welgene (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Sepmate, by STEMCELL (1 mentions)
Lymphoprep, by STEMCELL (1 mentions)
Growth factor reduced matrigel, by Corning (1 mentions)
E2 pellet, by Innovative Research (1 mentions)
Growth factor reduced matrigel, by BD (1 mentions)
Nod scid mice, by Orient Bio (1 mentions)
C3h hej mice, by Orient Bio (1 mentions)
Nightowl 2 lb983, by Berthold Technologies (1 mentions)
Cmc na, by Merck Group (1 mentions)
Rompun, by Bayer (1 mentions)
Zoletil 50, by Virbac (1 mentions)
Balb c nu nu mice, by Charles River Laboratories (1 mentions)
Matrigel, by BD (1 mentions)
Matrigel, by Merck Group (1 mentions)
Nightowl imaging system, by Berthold Technologies (1 mentions)
32 protocols using balb c nu nu mice
1
Xenograft Mouse Model for Cancer Research
2
Xenograft model of renal cancer
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The human renal cancer cell lines A498 and Caki-1 were obtained from the Korean Cell Line Bank. A498 and Caki-1 cells were cultured in Roswell Park Memorial Institute-1640 medium (WELGENE, Daegu, Republic of Korea) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Carlsbad, CA, USA) and 1% penicillin–streptomycin (Gibco). The cells were maintained in an incubator conditioned with 5% CO2 at 37 °C. Four-week-old female BALB/c nu/nu mice were purchased from ORIENT BIO. Animals were maintained at 23 ± 1 °C and 50 ± 10% humidity under specific pathogen-free conditions. The light–dark period was cycled every 12 h, and food and water were provided ad libitum. Animal experimental procedures were reviewed and approved by the CHA University Animal Care and Use Committee (IACUC210152).
3
Xenograft Model for MCF7 and ADR44P Cells
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MCF7 and ADR44P cells were grown overnight and harvested, washed with PBS. The suspension of 2.5 × 106 cells in 0.2 mL serum-free medium was injected subcutaneously into the flank of 6-weeks-old BALBc (nu/nu) mice (Orient Bio. Inc., Gyeonggi-do, Republic of Korea), which were maintained in pathogen-free environment. The tumor growth was measured from 7 to 14 weeks after inoculation of cells using calipers. Tumor volume was calculated by the formula V = (a2 × b)/2 (a is the width and b is the length in mm, respectively). Animal experiment was approved by the Institutional Ethics Committee on Animal Care and Experimentation at The Catholic University of Korea (approval number: 2016–035)
4
Assessing NTP-Induced Blood Cell Damage
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BALB/c nu/nu mice were purchased from Orient Bio Co. Ltd. (SungNam, Korea). Six to eight weeks old female mice were used in this study. Mouse-related experimental procedures and mouse handling were conducted in accordance with the Committee for Ethics in Animal Experiments of the Ajou University School of Medicine (AUMC, IACUC No. 2015-0030). Before use, mice were allowed to acclimatize for at least three days. Mice (n = 6) received 200 μL of NTP-treated saline by intravenous (IV) injection once daily for 2 weeks. Control mice (n = 6) received 200 μL of saline by the same route.
For analysis of blood cell damage by NTS, mouse peripheral blood mononuclear cells (PBMCs) were isolated from the heart using lymphoprep and SepMate (StemCell Technologies) following the manufacturer’s protocol. The SepMate tubes were centrifugated at 1200× g for 10 minutes. The mouse PBMCs were then resuspended in DMEM medium containing 10% FBS. Blood cells cytotoxicity was measured by PI staining. For PI-positive control, 100 μM H2O2 was treated at 37 °C for 1 hour.
For analysis of blood cell damage by NTS, mouse peripheral blood mononuclear cells (PBMCs) were isolated from the heart using lymphoprep and SepMate (StemCell Technologies) following the manufacturer’s protocol. The SepMate tubes were centrifugated at 1200× g for 10 minutes. The mouse PBMCs were then resuspended in DMEM medium containing 10% FBS. Blood cells cytotoxicity was measured by PI staining. For PI-positive control, 100 μM H2O2 was treated at 37 °C for 1 hour.
5
Nude Mice Xenograft Model for NTP Therapy
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Sixteen male BALB/c nu/nu mice were purchased from Orient Bio Co. Ltd (SungNam, Korea). FaDu cells (1 × 106) resuspended in PBS were administered subcutaneously into the lower right flank of each mouse. Procedures and handling were conducted in accordance with the Committee for Ethics in Animal Experiments of the Ajou University School of Medicine. After 1 week, when tumors reached ∼150 mm in diameter, the mice were randomly divided into two groups (eight mice per group) and daily treatment of a single 20 s NTP, 1 cm apart from the upper margin of tumor was performed for 20 days. Tumors were measured using a sliding caliper two times per week, and the volumes (mm3) were calculated as described previously.18 (link) On day 21, the tumors were excised from the mice that were killed for caspase-3, Nox-3, and TUNEL assays.
6
Subcutaneous Tumor Xenograft Model
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Five-week-old female Balb/c nu/nu mice were purchased from Orient Bio (Sungnam, Korea). EI24 gRNA- and control gRNA-MIA PaCa-2 cells (5 × 106/100 μl PBS) were injected subcutaneously into the flanks of mice. Tumor size was measured using a caliper, and tumor volume was calculated on the indicated day using the following equation: volume = (length × width2)/2. All mice were maintained under specific pathogen-free conditions at the National Cancer Center Research Institute (NCCRI) Animal Facility. All animal experiments in this study were performed in accordance with the institutional guidelines of the NCCRI Animal Facility, an Association for Assessment and Accreditation of Laboratory Animal Care International-accredited institution. This protocol was reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) of NCCRI (No. NCC-18-410).
7
Xenograft Mouse Model for PTK7 in Cancer
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Four to five-week-old male immune-deficient athymic nude (BALB/c nu/nu) mice were purchased from Orient Bio Inc. (Gyeonggi, Korea). To investigate the effect of PTK7 on tumorigenesis in a xenograft mouse model, 1 × 106 KYSE-30 cells expressing either PTK7-FLAG or PTK7 shRNA (PTK7-KD-6433 and 6434) were resuspended in growth-factor-reduced Matrigel (Corning) and subcutaneously injected into the backs of mice. Tumor growth in each group was evaluated by measuring the tumor size twice per week using calipers (length × width × depth/2). Mice were sacrificed 6 weeks after cell injection. The xenograft tumors were recovered to measure tumor weight, fixed in formalin, and were paraffin embedded for histological and immunohistochemical analyses [60 (link)].
8
Subcutaneous Tumor Xenograft Protocol
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The SKOV-NC and SKOV-miR-206 cells were harvested, washed twice with 1× PBS, and suspended in a serum-free medium. A suspension with 2.5×106 cells in 0.2 ml serum-free medium was injected subcutaneously into the flank of 6-week-old BALB/c-nu/nu mice Orient Bio Inc. (Seongnam, South Korea). The tumor growth was monitored weekly by measuring two diameters of tumors with calipers for 8 weeks. The tumor volume was calculated by the formula V = (a2×b)/2, where a and b are the width and the length, respectively, of the tumor in millimeters. Each group contained four or five animals. This animal experiment was performed according to the institutional guidelines for the care and use of laboratory animals as adopted by the U.S. National Institutes of Health and the Catholic University Animal Care and Use Committee (approval number: 2016-015).
9
Xenograft Mouse Model for Cancer Research
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The animals were cared for in accordance with the Guide for the Care and Use of Laboratory Animals published by the United States National Institutes of Health. The protocols were also approved by the Institutional Animal Care and Use Committee (IACUC) of Sungkyunkwan University (IACUC number: 202106291, approval date 15 July 2021). The BALB/c nu/nu mice (female, 6 weeks old) were purchased from ORIENT BIO (Seongnam, South Korea). Mice were housed in microisolator cages on individually ventilated cage racks with ad libitum access to an autoclaved standard rodent diet (LabDiet 5008, Purina, St. Louis, MO, USA) and were kept under a 12 h light/dark cycle.
10
Rottlerin Inhibits Ishikawa Cell-Derived Xenograft Tumors
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Approximately 5-week-old female Balb Cnu/nu mice were purchased from Orientbio Inc. (Seongnam, Korea). Mice were maintained as previously described [42 (link)]. An E2 pellet (1.7 mg/60-day release; Innovative Research of America) was implanted subcutaneously before the injection of Ishikawa cells. Five days later, Ishikawa cells (1.5×107) in 100 μL of DMEM/F12:Matrigel (1:1) were subcutaneously injected into the dorsal flank of each mouse. Two days later, 5 mg/kg of rottlerin in DMSO was administered intraperitoneally to the mice daily for 22 days, and the control mice were given DMSO in the same manner. Tumor volumes and body weights of mice were measured at every 3 days. Tumors were measured with Vernier calipers, and tumor volumes were calculated by the formula π/6×length×width×height. The mice were sacrificed under anesthesia, and the tumors were collected for further analysis.
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