Two stimulating tests were performed to assess growth hormone (GH) secretion (the first one, 500 mg of levodopa for those weighing more than 30 kg; 250 mg of levodopa for those weighing less than 30 kg, orally; and the second one, 0.1 U/kg insulin, subcutaneously). GH concentration measurements were performed at 0, 30, 60, 90, and 120 min after administration. A chemiluminescence method was used to assess the GH concentration (ACCESS2, Beckman Coulter; USA), and the sensitivity was 0.010 μg/l. Insulin-like growth factor-1 (IGF-1) and IGF-binding protein-3 (IGFBP-3) were measured by a chemiluminescence assay (DPC IMMULITE 1000 analyser, SIEMENS, Germany) with intra- and interassay CVs of 3.0% and 6.2% for IGF-1 and 4.4% and 6.6% for IGFBP-3, respectively. A GH peak in the GH stimulation test of less than 10 ng/ml was defined as GHD [18 (link)]. The IGF-1 SDS for age and sex was calculated according to IGF-1 levels for healthy children and adolescents of the same age and sex [19 (link)].
Dpc immulite 1000 analyser
Manufactured by Siemens
Sourced in Germany
The DPC IMMULITE 1000 analyser is a diagnostic instrument used for the quantitative determination of various analytes in biological samples, such as blood or serum. It utilizes a solid-phase, enzyme-labeled, chemiluminescent immunoassay technology to perform automated immunoassay testing.
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Lab products found in correlation
5 protocols using dpc immulite 1000 analyser
1
Growth Hormone Secretion Assessment
2
Measurement of Serum IGF-1 and IGFBP-3
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Serum IGF-1 and IGFBP-3 levels were measured by the chemiluminescence immunometric method (DPC IMMULITE 1000 analyser, SIEMENS, Germany) with intra- and interassay CVs for IGF-1 of 3.0% and 6.2%, respectively, and intra- and interassay CVs for IGFBP-3 of 4.4% and 6.6%. The IGF-1 standard deviation score (IGF-1 SDS) adjusted for age and sex was determined according to IGF-1 levels of healthy Japanese children and adolescents of the same age and sex.23 (link) Liver function (including alanine aminotransferase (ALT) and AST levels), renal function (including Cr, blood urea nitrogen (BUN), and uric acid (UA)), blood lipid indices (including total cholesterol (TC), high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein C (LDL-c), triglycerides (TGs), and fasting blood glucose (FBG)), and serum calcium and phosphorus were measured by a biochemical automatic analyser (Cobas c702, Roche; Shanghai, China).
3
Metabolic and Hormonal Biomarkers Assessment
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Laboratory parameters were measured from fasting blood samples collected from all participants. A biochemical automatic analyser was used to detect the following indexes: blood lipids—TG, total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and LDL-C and renal function—creatinine (Cr), urea nitrogen (BUN) and SUA (Cobas c702, Roche; Shanghai, China). A chemiluminescence assay with intra-assay and inter-assay parameters with variations of 3.0 and 6.2%, respectively (DPC IMMULITE 1000 analyser, SIEMENS, Berlin, Germany), was performed to detect serum IGF-1 levels. IGF-1 SDS got counted by IGF-1 degrees derived from age- and sex-matched robust children and teenagers [36 (link)].
4
Serum IGF-1 and Metabolic Profiles in Children
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Fasting blood samples were obtained from all participants to measure the serum IGF-1 level and other laboratory parameters.
Serum IGF-1 concentrations were estimated based on a chemiluminescence assay (DPC IMMULITE 1000 analyser, SIEMENS, Germany) with an intra-assay and inter-assay coefficient of variation of 3.0 and 6.2%, respectively; the IGF-1 SDS, adjusted for age and sex, was calculated according to IGF-1 levels of healthy Japanese children and adolescents of the same age and sex [39 (link)]. Total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), LDL-C, very low density lipoprotein cholesterol (VLDL-C), triglyceride (TG), alanine aminotransferase (ALT), fasting plasma glucose (FPG) and uric acid (UA) levels were determined using an auto biochemical analyser (Cobas c702, Roche; Shanghai, China). LDL-C dyslipidaemia was defined according to an expert consensus regarding Chinese child and adolescent dyslipidaemia [40 ].
Serum IGF-1 concentrations were estimated based on a chemiluminescence assay (DPC IMMULITE 1000 analyser, SIEMENS, Germany) with an intra-assay and inter-assay coefficient of variation of 3.0 and 6.2%, respectively; the IGF-1 SDS, adjusted for age and sex, was calculated according to IGF-1 levels of healthy Japanese children and adolescents of the same age and sex [39 (link)]. Total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), LDL-C, very low density lipoprotein cholesterol (VLDL-C), triglyceride (TG), alanine aminotransferase (ALT), fasting plasma glucose (FPG) and uric acid (UA) levels were determined using an auto biochemical analyser (Cobas c702, Roche; Shanghai, China). LDL-C dyslipidaemia was defined according to an expert consensus regarding Chinese child and adolescent dyslipidaemia [40 ].
5
Evaluation of Insulin-Related Biomarkers
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Following an 8–12-h period of fasting, fasting blood samples were obtained from all participants for laboratory analyses. Serum IGF-1 concentrations were estimated based on a chemiluminescence assay (DPC IMMULITE 1000 analyser, SIEMENS, Germany) with intra-assay and inter-assay coefficients of variation of 3.0 and 6.2%, respectively. Total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), triglyceride (TG), fasting plasma glucose(FPG), and UA levels were determined using an auto biochemical analyser (Cobas c702, Roche; Shanghai, China). Glycated haemoglobin (HbA1c) concentration was measured using a high-performance liquid chromatography method. Plasma glucose was measured using a hexokinase method. Plasma insulin concentrations were determined using a chemiluminescent immunoassay.
Insulin resistance (IR) was estimated by homeostasis model assessment for insulin resistance (HOMA-IR) = FPG (mmol/L)×fasting plasma insulin (FINS)(μIU/mL)/22.5. The standard deviation score of IGF-1 (IGF-1 SDS) was calculated according to a previous study.21 (link)
Insulin resistance (IR) was estimated by homeostasis model assessment for insulin resistance (HOMA-IR) = FPG (mmol/L)×fasting plasma insulin (FINS)(μIU/mL)/22.5. The standard deviation score of IGF-1 (IGF-1 SDS) was calculated according to a previous study.21 (link)
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