Most drugs were dissolved in water to make stock solutions (typically 10,000x concentrations) that were frozen as aliquots and used as needed during experiments. Acetylcholine (Fisher Scientific) was bath-applied with physostigmine hemisulfate (eserine; Tocris Bioscience), a blocker of Acetylcholinesterase. Atropine and pirenzepine dihydrochloride were purchased from Sigma-Aldrich. Tetrodotoxin (TTX) citrate and CGP 52432 were purchased from HelloBio, Inc. Clozapine N-oxide (CNO) dihydrochloride was purchased from Tocris Biosciences. SR-95531 (gabazine) was purchased from MedChemExpress and dissolved in DMSO for stock solutions.
Physostigmine hemisulfate eserine
Manufactured by Bio-Techne
Physostigmine hemisulfate is a cholinesterase inhibitor that acts as a reversible acetylcholinesterase inhibitor. It is a physiologically active component derived from the Calabar bean.
Automatically generated - may contain errors
2 protocols using physostigmine hemisulfate eserine
1
Pharmacological Modulation of Neurotransmission
2
Pharmacological Reagents for Neuroscience
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Most drugs were dissolved in water to make stock solutions (typically at 10,000× concentration) that were frozen as aliquots and used as needed during experiments. ACh (Fisher Scientific) was bath-applied with physostigmine hemisulfate (eserine; Tocris Bioscience), a blocker of acetylcholinesterase (AChE). Atropine and pirenzepine dihydrochloride were purchased from Sigma-Aldrich. TTX citrate and CGP 52432 were purchased from HelloBio, Inc. CNO dihydrochloride, DNQX, and D-AP5 were purchased from Tocris Bioscience. SR-95531 (gabazine) was purchased from MedChemExpress and dissolved in DMSO for stock solutions.
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