Prominence lc 20ap

Matrix-Assisted Laser Desorption/ Ionization Time of Flight Mass Spectrometry (MALDI TOF-MS) spectroscopy was performed on a Bruker Autoflex TOF/TOF using α-cyano- 4-hydroxy cinnamic acid as a matrix. Ultraviolet/visible spectra were obtained from a Hitachi U-2900 spectrophotometer. The fluorescence spectra were obtained from a FL-5301PC (Shimadzu) fluorescence spectrophotometer. Circular dichroism spectra were obtained using Jasco J-810 spectropolarimeter. The AFM measurements were conducted on a MultiMode 8 AFM with NanoScope V controller, NanoScope software and NanoScope Analysis software (Bruker AXS Corporation, Santa Barbara, CA, USA) in air at ambient temperature (ca. 25 °C) in the tapping mode. Images were acquired in PeakForce Tapping mode. X-ray diffraction patterns were obtained using a Rigaku D/max 2550 diffractometer (Rigaku Co.). HPLC analysis was performed with Prominence LC-20AP (Shimadzu) and Vydac C18 reverse phase column (218TP54). Molecular modelling and calculations were performed by Material Studio 6.0 programme (Accelrys Software Inc.) and the MacroModel module within the molecular modelling suite Maestro from Schrödinger Suites (Schrödinger K.K.).

Dried Nelumbo nucifera leaves were purchased from a local pharmacy. Extraction of Q3G from the leaves was performed according to the method stipulated by Ohara et al.35 (link) with modifications. Briefly, the dried leaves (100 g) were extracted with 2 L of 50% aqueous ethanol at room temperature for 12 h. The extract was filtered, and then concentrated under reduced pressure. The concentrate was lyophilized, redissolved and then fractionated by HPLC apparatus (Shimadzu, Prominence LC-20AP, Japan) consisting of the following: A LC pump equipped with a LC-20 UV/Vis detector and a LC-8A column holder using YMC-Pack ODS-A columns (250 mm × 20 mm, S-5 μm, 12 nm). The mobile phase included 25% acetonitrile and Ultra-pure water containing 0.1% formic acid.