miR-29b agomir (2′OME+5′chol modified) and negative control agomir (2′OME+5′chol modified) (RiboBio) were used. Intramuscular injection (25 nmol per mice) was performed once a day for 3 days. After 4 days, all mice were killed and gastrocnemius muscles were removed, weighed, frozen and stored in 4% PFA. The experiments were blindly performed by the investigator who did not know the group allocation.
Mir 29b agomir
Manufactured by RiboBio
Sourced in China
MiR-29b agomir is a synthetic RNA molecule designed to mimic the function of the endogenous microRNA miR-29b. MicroRNAs are small, non-coding RNA molecules that play a crucial role in regulating gene expression. The core function of MiR-29b agomir is to modulate the expression of target genes, which may be involved in various biological processes.
Automatically generated - may contain errors
3 protocols using mir 29b agomir
1
miR-29b Agomir Intramuscular Injection
2
Transfection of miR-29b agomir in SKOV3 cells
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Stabilized miRNAs (miR-29b agomir and NC agomir) were purchased from RiboBio (Guangzhou, China). SKOV3 cells were transfected with 100 nM of miR-29b agomir or NC agomir (stabilized miRNA) by nucleofection with program T-028 of Nucleofector II (Amaxa Biosystems, Gaithersburg, MD, USA). After the transfection, the cells were incubated in high-glucose DMEM for 24 h at 37°C for recovery. Then, the cells were collected and washed with ice-cold 1 × PBS three times and suspended in 1 × PBS at a concentration of 1 × 107 cells/ml for subsequent animal injections.
3
Astragaloside IV and Ferulic Acid Assay
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Astragaloside IV (purity ≥98%) and ferulic acid (purity≥98%) were bought from Shanghai yuan ye Bio-Technology Co., Ltd. (Shanghai, China). MIR-29 b agomir and agomir negative control were bought from Guangzhou Ribobio Co., Ltd. (Guangzhou, China). The assay kits of MDA, ROS, and SOD for mice were purchased from Jiancheng Bioengineering Institute (Nanjing, China). The HYP kit was bought from Nanjing Jiancheng Bioengineering Co., Ltd. (Nanjing, China).
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