Plasma glucose was measured in the Clinical Research Center by the glucose oxidase method (Analox Glucose Analyzer, Analox Instruments, Lunenburg, MA). For other analyses, blood samples were placed on ice at the bedside, processed within 15–20 minutes, and frozen at −80°C until the samples were analyzed. Blood samples for the analysis of drug concentration were collected prior to the morning dose, and it was assured that patients were on chronic pioglitazone and that the drug concentration was at steady-state. A novel liquid chromatography tandem mass spectrometry assay described previously was used to measure plasma concentrations of pioglitazone, hydroxypioglitazone, and ketopioglitazone.17 The homeostasis model assessment of insulin resistance (HOMA-IR) was calculated from a single measurement of fasting insulin and glucose. Plasma insulin was determined by radioimmunoassay (Siemens, Los Angeles, CA). Adiponectin concentrations were measured by magnetic bead Milliplex technology (Luminex xMAP, Millipore, St. Charles, MO), and CK-18 levels were quantified by ELISA (M30-Apoptosense, DiaPharma, Columbus, OH).
M30 apoptosense
Manufactured by Diapharma
The M30-Apoptosense is a laboratory equipment product that measures the level of cytokeratin-18 (CK-18) in biological samples. CK-18 is a protein that is released during apoptosis, or programmed cell death. The M30-Apoptosense provides a quantitative assessment of apoptosis, which can be useful in various research and diagnostic applications.
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Lab products found in correlation
2 protocols using m30 apoptosense
1
Quantification of Pioglitazone and Metabolites
2
Noninvasive Markers of Liver Fibrosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Changes from baseline in noninvasive markers of fibrosis, including the Enhanced Liver Fibrosis test (Siemens, Tarrytown, NY) and FibroSure/FibroTest (LabCorp, Burlington, NC), were assessed, as well as changes in markers of liver injury and function, including serum ALT, aspartate aminotransferase (AST), bilirubin, gamma‐glutamyltransferase, and alkaline phosphatase. Changes from baseline in serum levels of cytokeratin‐18 M30 and M65 fractions were measured as indicators of hepatocellular apoptosis and necrosis, respectively, using the M30 Apoptosense and M65 EpiDeath enzyme‐linked immunosorbent assays (Diapharma, West Chester, OH).
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