Suspensions of n-ZnO (mean particle size 35 nm), reduced glutathione (GSH), bovine serum albumin, phenylmethylsulfonyl fluoride (PMSF), 5,5′-dithio-bis(2-nitrobenzoic acid) (DTNB), nicotinamide adenine dinucleotides (NADH, NAD, NADPH), EDTA, dihydrorhodamine, Hoescht 33342 dye, 2,4-Dinitrophenylhydrazine, tyrosin, hemoglobin, chymotrypsinogen, cytohrome c, myoglobin, ubiquitin, Sephadex G-50, β-mercaptoethanol, ethoxyresorufin, certified reference material ERM-BB422 and Lactobacillus leichmannii D-Lactate dehydrogenase were purchased from Sigma Chem. Co. (St. Louis, USA). All other chemicals were obtained from the Synbias (Kyiv, Ukraine), Bayer (Kyiv, Ukraine) and Balkanpharma-Dupnitsa (Dupnitsa, Bulgaria) commercial suppliers. All reagents were of the analytical grade or higher.
Hoescht 33342 dye
Manufactured by Merck Group
Hoechst 33342 dye is a fluorescent stain used in biological research. It binds to the minor groove of double-stranded DNA, allowing for the visualization and quantification of DNA content in cells. The dye exhibits blue fluorescence when bound to DNA, making it a useful tool for applications such as cell cycle analysis and DNA content determination.
Automatically generated - may contain errors
Lab products found in correlation
Chymotrypsinogen, by Merck Group (1 mentions)
Dihydrorhodamine, by Merck Group (1 mentions)
Ethoxyresorufin, by Merck Group (1 mentions)
Hemoglobin, by Merck Group (1 mentions)
Tyrosin, by Merck Group (1 mentions)
Myoglobin, by Merck Group (1 mentions)
N zno, by Merck Group (1 mentions)
Sephadex g 50, by Merck Group (1 mentions)
2 4 dinitrophenylhydrazine, by Merck Group (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
β mercaptoethanol, by Merck Group (1 mentions)
Phenylmethylsulfonyl fluoride, by Merck Group (1 mentions)
5 5 dithiobis 2 nitrobenzoic acid, by Merck Group (1 mentions)
Ubiquitin, by Merck Group (1 mentions)
Reduced glutathione, by Merck Group (1 mentions)
Live dead cell imaging kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using hoescht 33342 dye
1
Zinc Oxide Nanoparticle Assays
2
Viability Assessment of hMSCs and MOSJ Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A Live/Dead Cell Imaging Kit (Life Technologies) was employed to assess the viability of both the hMSCs and MOSJ cells on the beads. The assay was carried out according to the manufacturer’s instructions with the exception of the use of Hoescht 33342 dye (Sigma) to visualize dead MOSJ cells as it was not possible to distinguish between the propidium iodide stain and the dsRed2 already expressed by the cells. Live staining was carried out on hMSCs not expressing GFP to allow visualization of the calcein AM dye that indicates live cells.
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