Anti cd38 pb

Manufactured by BioLegend

Anti-CD38-PB is a mouse monoclonal antibody that recognizes the CD38 antigen. CD38 is a transmembrane glycoprotein expressed on various cell types, including lymphocytes and plasma cells. This antibody is conjugated to Pacific Blue, a fluorescent dye that can be used in flow cytometry applications.

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Lab products found in correlation

Anti cd95 pecy7, by BioLegend (1 mentions) Streptavidin af647, by BioLegend (1 mentions) 70 mm cell strainer, by BD (1 mentions) Ack lysing buffer, by Thermo Fisher Scientific (1 mentions) Lsrfortessa, by BD (1 mentions) Anti b220 bv605, by BioLegend (1 mentions) Anti igd bv786, by BioLegend (1 mentions) Anti cd138 bv421, by BioLegend (1 mentions) Anti cd4 ef780, by Thermo Fisher Scientific (1 mentions) Anti cd8 ef780, by Thermo Fisher Scientific (1 mentions) Anti nk1.1 ef780, by Thermo Fisher Scientific (1 mentions) Anti f4 80 ef780, by Thermo Fisher Scientific (1 mentions) Anti cd16 32, by BD (1 mentions) Anti cd4 apc efluor 780, by Thermo Fisher Scientific (1 mentions) Tcl buffer, by Qiagen (1 mentions) Zombie nir, by BioLegend (1 mentions) Mouse fc block, by BD (1 mentions) Accucheck counting beads, by Thermo Fisher Scientific (1 mentions) F4 80, by BD (1 mentions) 2 β mercaptoethanol, by Merck Group (1 mentions)

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Anti-CD38 (12 citations)

2 protocols using anti cd38 pb

Phenotyping Lymphocyte Populations by Flow Cytometry

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Lymph nodes (axillary or popliteal) were collected in FACS buffer (PBS1X, 10%FCS, 2mM EDTA) on ice. Single-cell suspensions were obtained by mechanical disruption through a 70-mm cell strainer (BD Biosciences). Erythrocytes were lysed with 1 ml of ACK lysing buffer (Gibco). After incubation with 5 μg/ml of anti-CD16/32 (rat mAb 2.4G2, mouse Fc block BD) for 15 min at 4°C, cell surface antigens were stained for 30 min at 4°C. When a biotin antibody was used, the cells were then incubated with a streptavidin-fluorophore conjugate for 20 min at 4°C. Flow cytometric analysis was performed on a BD LSRFortessa. Antibodies used: from BD; Anti-IgM-e710 (R6-60.2, 550881), Anti-IgD-BV786 (11-26c.2a, 563618), Anti- T-and B-cell activation antigen-FTIC (GL7, 553666), Anti-Igλ1,2,3-BV650 (R26-46, 744526), Anti-CD95-PE-Cy7 (Jo2, 557653) streptavidin-BV711 (563262), streptavidin-AF647, from Biolegend; Anti-CD38-PB (90, 102719), Anti-CD138-BV421 (281-2, 142508), Anti-B220-BV605 (RA3-6B2, 103244) live/dead marker Zombie NIR (Biolegend, 423106) and from eBiosciences; Anti-T-and B-cell activation antigen-e660 (GL7, 50-5902-82), Anti-CD4-eF780 (RM4-5, 47-0042-82), Anti-CD8-eF780 (53-6.7, 47-0081-82), Anti-NK1.1-eF780 (PK136, 47-5941-82), Anti-F4/80-eF780 (BM8, 47-4801-82), Anti-TM4-Core-biot (5μg/mL) provided by A.T.M and L.S (Fred Hutchinson Cancer Research Center, Seattle).

Viant C., Weymar G.H., Escolano A., Chen S., Hartweger H., Cipolla M., Gazumayan A, & Nussenzweig M.C. (2020). Antibody affinity shapes the choice between memory and germinal center B cell fates. Cell, 183(5), 1298-1311.e11.

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Isolation of Germinal Center B Cells

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Popliteal lymph nodes from mice were collected in FACS buffer (1× PBS, 2% FBS, 2 mM EDTA), mechanically disrupted and filtered through a 35 µM strainer (Corning, 352235). Cells then underwent iterative rounds of staining each for 20 min on ice (all of the antibodies were diluted at 1:200 unless stated otherwise): (1) anti-mouse CD16/32 (Mouse BD FC Block, BD Biosciences, 553142); (2) fluorophore-conjugated RBD (see above); (3) anti-T and anti-B cell activation antigen-FITC (BD Biosciences, 553666), anti-CD38-PB (BioLegend, 102720; 1:100 dilution), anti-CD45R/B220-BV605 (BioLegend, 103244), anti-CD4-APC-eFluor780 (Invitrogen, 47-0042-82), anti-CD8a-APC-eFluor780 (Invitrogen, 47-0081-82), anti-NK1.1-APC-eFluor780 (Invitrogen, 47-5941-82), anti-F4/80-APC-eFluor780 (Invitrogen, 47-4801-82), anti-CD95-PE-Cy7 (BD Biosciences, 557653) and Zombie NIR (BioLegend, 423105, 1:1,000). AccuCheck Counting Beads (Life Technologies, PCB100) were added to samples according to the manufacturer’s instructions. Single CD4⁻CD8a⁻NK1.1⁻F4/80⁻B220⁺CD38⁻GL7⁺CD95⁺ cells were sorted on the BD FACSymphony S6 system into 96-well plates containing 1% 2-β-mercaptoethanol (Sigma-Aldrich) in TCL Buffer (Qiagen, 1031576) and subsequently frozen on dry ice and stored at −80 °C for RNA reverse transcription.

Schaefer-Babajew D., Wang Z., Muecksch F., Cho A., Loewe M., Cipolla M., Raspe R., Johnson B., Canis M., DaSilva J., Ramos V., Turroja M., Millard K.G., Schmidt F., Witte L., Dizon J., Shimeliovich I., Yao K.H., Oliveira T.Y., Gazumyan A., Gaebler C., Bieniasz P.D., Hatziioannou T., Caskey M, & Nussenzweig M.C. (2022). Antibody feedback regulates immune memory after SARS-CoV-2 mRNA vaccination. Nature, 613(7945), 735-742.

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