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Yeast extract

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Yeast extract is a food ingredient derived from the autolysis of yeast cells. It is a rich source of amino acids, vitamins, and other nutrients essential for microbial growth and development. Yeast extract serves as a key component in various media formulations for culturing microorganisms in laboratory settings.

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Lab products found in correlation

Soytone, by BD (3 mentions) Yeast extract, by BD (3 mentions) Soytone, by Teknova (3 mentions) Trace element mix, by Teknova (2 mentions) E coli strain bl21, by New England Biolabs (1 mentions) Tryptone, by Teknova (1 mentions)

5 protocols using yeast extract

1

Scalable XTEN Protein Expression

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All of the thiol-containing XTEN precursors
(XTEN-1, 2, 3, 4) were expressed in the BL21 E. coli strain (New England Biolabs, #C2530H) using 5 L B. Braun Biostat
B glass-jacketed fermentation vessels. 125 mL starter cultures were
used to inoculate 1.7 L batches of fermentation media containing 50
mM (NH4)2SO4, 20 mM K2HPO4, 15 mM KH2PO4, 4.5 mM C6H5Na3O7·2H2O, 11 mM NaH2PO4, 10 mM MgSO4, 30
g/L NZ BL4 soy peptone (Kerry Bioscience, #5X00043), 15 g/L yeast
extract (Teknova, #Y9020), 0.25 mL/L polypropylene glycol 2000, trace
elements, and 10 mg/mL tetracycline. Salt feeds containing 75 mM (NH4)2SO4, 150 mM K2HPO4, 110 mM KH2PO4, 7 mM C6H5Na3O7·2H2O, and 100 mM NaH2PO4 were started after 6 h at 25 g/h and continued
for 8 h. Cultures were grown at 37 °C for 17 h before shifting
the temperature to 26 °C and adding 27 mL of 1 M MgSO4. The carbon source consisted of a 70% glycerol feed, with roughly
2 L being fed over the course of the run. After 48 h, the cultures
were harvested by centrifugation, yielding cell pellets of approximately
1 kg by wet weight. The pellets were stored at −80 °C
until purification was commenced.
Ding S., Song M., Sim B.C., Gu C., Podust V.N., Wang C.W., McLaughlin B., Shah T.P., Lax R., Gast R., Sharan R., Vasek A., Hartman M.A., Deniston C., Srinivas P, & Schellenberger V. (2014). Multivalent Antiviral XTEN–Peptide Conjugates with Long in Vivo Half-Life and Enhanced Solubility. Bioconjugate Chemistry, 25(7), 1351-1359.
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2

Chronic Wound Biofilm in db/db Mice

Cited 1 time
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Chronic wounds were made in db/db−/− mice as previously described [24 (link)]. Six to seven-month-old db/db−/− mice were treated intraperitoneally with a catalase inhibitor, 3-amino-1,2,4-triazole, at 1 g/kg body weight before wounding. Immediately after wounding, they were treated once topically with the glutathione peroxidase inhibitor, mercaptosuccinic acid, at 150 mg/kg body weight. Using our procedure, the wounds are fully chronic in 20 days and contain abundant biofilm. All bacteria forming the biofilm are from the mouse natural skin with no additional manipulation or introduction of laboratory-carried strains; therefore, the biofilm forms naturally from the microbiome in the skin. For these studies, the biofilms were collected using the Levine method with sterile cotton swabs and stored for bacteria identification both dry at -80°C and in Luria-Bertani (LB) media (10 g tryptone, 5 g yeast extract, and 10 g NaCl and Milli-Q water to 1 L; Teknova) supplemented with 20% glycerol. Swabs in LB media with 20% glycerol were then cultured in fresh LB media overnight at 37°C (150 rpm) and aliquots stored at -80°C.
Li X., Kim J., Wu J., Ahamed A.I., Wang Y, & Martins-Green M. (2020). N-Acetyl-cysteine and Mechanisms Involved in Resolution of Chronic Wound Biofilm. Journal of Diabetes Research, 2020, 9589507.
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3

Bacterial Growth Media Preparation

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For pre-cultures of the bacteria the basic buffer recipe was 10g/L yeast extract (Becton Dickinson, Franklin Lakes, USA) and 10g/L soytone (Becton Dickinson, Franklin Lakes, USA). We refer to that buffer as 1xNutrient medium (also 1xNu). The initial pH was 7 and 100mM phosphate were added. For the washing steps and the experiments itself the medium contained 1g/L yeast extract and 1g/L soytone, 0.1mM CaCl2, 2mM MgCl2, 4mg/L NiSO4, 50mg/L MnCl2 and 1x Trace Element Mix (Teknova, Hollister, USA). We refer to that buffer as base buffer. It was supplemented with phosphate buffer and/or glucose as outlined in the single experiments. The usual concentration was 10g/L glucose, deviations from that are described for the single experiments below. All media were filter sterilized.
Ratzke C., Denk J, & Gore J. (2018). Ecological suicide in microbes. Nature ecology & evolution, 2(5), 867-872.
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4

Bacterial Growth Media Preparation

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For pre-cultures of the bacteria the basic buffer recipe was 10g/L yeast extract (Becton Dickinson, Franklin Lakes, USA) and 10g/L soytone (Becton Dickinson, Franklin Lakes, USA). We refer to that buffer as 1xNutrient medium (also 1xNu). The initial pH was 7 and 100mM phosphate were added. For the washing steps and the experiments itself the medium contained 1g/L yeast extract and 1g/L soytone, 0.1mM CaCl2, 2mM MgCl2, 4mg/L NiSO4, 50mg/L MnCl2 and 1x Trace Element Mix (Teknova, Hollister, USA). We refer to that buffer as base buffer. It was supplemented with phosphate buffer and/or glucose as outlined in the single experiments. The usual concentration was 10g/L glucose, deviations from that are described for the single experiments below. All media were filter sterilized.
Ratzke C., Denk J, & Gore J. (2018). Ecological suicide in microbes. Nature ecology & evolution, 2(5), 867-872.
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5

Bacterial Culture Media Composition

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For precultures of the bacteria, the basic buffer recipe was 10 g/L yeast extract (Becton Dickinson, Franklin Lakes, NJ) and 10 g/L soytone (Becton Dickinson). We refer to that buffer as 1xNutrient medium. For the washing steps and the experiment itself, the medium contained 1 g/L yeast extract and 1 g/L soytone, 0.1 mM CaCl2, 2 mM MgCl2, 4 mg/L NiSO4, 50 mg/L MnCl2, and 1x Trace Metals Mixture (Teknova, Hollister, CA). We refer to that buffer as base buffer. For the different bacteria, the initial pH was either 6 or 7, and it was supplemented with phosphate buffer and/or glucose and urea as outlined in the specific experiments below. The glucose and urea were added freshly every day directly before starting the experiments, to avoid degradation of the urea. The usual concentrations were 10 g/L glucose and 8 g/L urea; deviations from that are described for the single experiments below.
All media were filter sterilized.
Ratzke C, & Gore J. (2018). Modifying and reacting to the environmental pH can drive bacterial interactions. PLoS Biology, 16(3), e2004248.
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