Human normal liver epithelial cells (THLE-3) and HCC cell lines SKHEP1 and SNU449 were purchased from American Type Culture Collection. HCC cell lines Li7 and Huh7 were purchased from RIKEN BioResource Center. THLE-3 cells were cultured in 90% bronchial epithelial growth medium (Lonza Group, Ltd.) and 10% FBS (Gibco; Thermo Fisher Scientific, Inc.). Li7 cells and SNU449 cells were cultured in 90% RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) and 10% FBS. Huh7 cells were cultured in 90% DMEM (Gibco; Thermo Fisher Scientific, Inc.) and 10% FBS. SKHEP1 cells were cultured in 90% Eagle's minimum essential medium (Gibco; Thermo Fisher Scientific, Inc.) and 10% FBS. All medium was added 100 U/ml penicillin and 100 lg/ml treptomycin (Sigma-Aldrich; Merck KGaA). Cells were maintained in a humid incubator at 37˚C.
Treptomycin
Manufactured by Merck Group
Sourced in United States
Treptomycin is a laboratory equipment product manufactured by Merck Group. It is a precision instrument designed for use in scientific research and analysis. The core function of Treptomycin is to facilitate accurate and reliable measurements in various laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Merck Group (2 mentions)
Sk hep1, by Thermo Fisher Scientific (1 mentions)
Snu449, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Bronchial epithelial growth medium (begm), by Lonza (1 mentions)
Sk hep1, by American Type Culture Collection (1 mentions)
Thle 3, by American Type Culture Collection (1 mentions)
Snu 449, by American Type Culture Collection (1 mentions)
Thle 3, by Lonza (1 mentions)
Glutamine, by Merck Group (1 mentions)
Non essential amino acids (neaa), by Merck Group (1 mentions)
Htb 14, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
2 protocols using treptomycin
1
Culturing Human Liver Cell Lines
2
Culturing Human Glioblastoma Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human glioblastoma cell lines (U251MG and U87MG) (ATCC® HTB-14™) were cultured in Dulbecco Modified Eagle Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, St. Louis, MO, USA), 50 µg/mL treptomycin, 50 IU/mL penicillin, 2 mM glutamine, 1% non-essential amino-acids (Sigma-Aldrich, St. Louis, MO, USA) and maintained at 37 °C, in an atmosphere of 90% air/10% CO2.
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