Anti lamin a c sab4200236

A monoclonal antibody against E-selectin (clone 7A9) was obtained from the American Type Culture Collection; anti-E-selectin (A-10: sc-137203) and anti-NF-kB p65 (C-20: sc-372) antibodies were obtained from Santa Cruz Biotechnology; anti-phospho-NF-κB p65 Ser 536 (#3031), anti-SAPK/JNK (#9252), anti-p38 MAPK (#9212), anti-phospho-SAPK/JNK (#9251), and anti-phospho-p38 MAPK (#4511) antibodies were obtained from Cell Signaling Technology; anti-lamin A/C (SAB4200236) and anti-actin (A5060) antibodies were obtained from Sigma-Aldrich; an anti-α-tubulin antibody (PM054-7) was obtained from Medical & Biological Laboratories; an anti-acetyl-histone H3 antibody (06-599) was obtained from Millipore; an Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (A11017) was obtained from Life Technologies; horseradish peroxidase (HRP)-linked anti-mouse (NA931V) and anti-rabbit (NA9340V) secondary antibodies were obtained from GE Healthcare.

Western blot analysis was performed according to the standard procedure. Anti-Lamin B1 (66095), β-actin (01003), P16 (10883), and P21 (10355) were purchased from proteintech (Wuhan, Hubei, China). Anti-Lamin A/C (SAB4200236) was purchased from sigma (Shanghai, China). Both γ-H2AX (ab2893) and H3K9me3 (ab8898) were purchased from abcam (Shanghai, China). P53 (9284S) was purchased from CST (Shanghai, China). SDS–polyacrylamide gels were used to separate the samples, and then tansfers were done onto nitrocellulose (NC) membranes. Corresponding antibodies were used to incubate the membranes. ECL Luminous Liquid (Millipore, China) was used to develop the target protein band and the images were analyzed using Image LabTM software (Bio-Rad, China).