Goat anti mouse and goat anti rabbit horseradish peroxidase conjugated igg

Cell lines. SKOV3, OVACAR5, MDA-MB-231 and MDA-MB-361 cells [from the American Tissue Culture Collection (ATCC) Manassas, VA, USA] were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum, and 1% streptomycin/penicillin at 37̊C in a humidified incubator containing 5% CO 2 in air.
Antibodies and chemical reagents. Mouse anti-β-actin antibody, dimethyl sulfoxide (DMSO), glycerol, glycine, sodium chloride, Trizma base and Tween-20 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mouse anti-PARP1, rabbit anti-FOXO3, mouse anti-LaminA/C antibodies and auranofin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti-Her3, rabbit anti-Her2, rabbit anti-phospho-FOXO3, rabbit anti-phospho-Akt, rabbit anti-Akt, rabbit anti-caspase-3, rabbit anti-Bax, rabbit anti-Bim and rabbit anti-Bcl2 antibodies were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). The rabbit anti-MUC4 antibody was purchased from Abcam (Cambridge, MA). Goat anti-mouse and goat anti-rabbit horseradish peroxidase-conjugated IgG were obtained from Jackson ImmunoResearch (West Grove, PA, USA). ECL Western Blotting Detection Reagents were obtained from GenDEPOT (Barker, TX, USA).