J774A.1 murine macrophages were plated at a density of 1×106cells/mL in a 12 well plate and allowed to adhere overnight. After which, the culture medium was replaced with 1 mL freshDMEM (Hyclone, Logan, UT, USA) with 10% FBS. Varying concentrations of GST-melittin (0.3 μM or 3 μM) or native melittin (0.3 μM) were added to the culture medium for one hour. Following this, the macrophages were stimulated with 10 μg/mL FITC-labeled LPS (Sigma Aldrich, St. Louis, MO, USA). Supernatant media was collected after a 4-hour incubation period and fluorescence was measured at excitation and emission wavelengths of 490 and 550 nm on Turner Quantech flourometer. LPS binding was compared to that of cells treated with FITC-labeled LPS, but not with the proteins, to determine the extent of inhibition of LPS binding.
Fitc labeled lps
Manufactured by Merck Group
Sourced in United States, Spain
FITC-labeled LPS is a fluorescently labeled version of lipopolysaccharide (LPS), a component of the outer membrane of Gram-negative bacteria. This product is used in research applications to study the interaction of LPS with cells or other biological systems.
Automatically generated - may contain errors
Lab products found in correlation
Anti gsdmd sc 393581, by Santa Cruz Biotechnology (1 mentions)
Tlrl pms, by InvivoGen (1 mentions)
Pam3csk4, by InvivoGen (1 mentions)
Dotap, by Roche (1 mentions)
Melittin from honey bee venom, by Merck Group (1 mentions)
Polystyrene microtiter plates, by Greiner (1 mentions)
Sigmafast opd, by Merck Group (1 mentions)
Lf from human milk, by Merck Group (1 mentions)
Bovine serum albumin heat shock fraction, by Merck Group (1 mentions)
3 protocols using fitc labeled lps
1
Modulation of LPS Binding in Macrophages
2
Investigating Inflammatory Signaling Pathways
3
Protein-based ELISA Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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FITC-labeled LPS from Escherichia coli serotypes O111:B4 and O55:B5, PMX sulphate salt, ovalbumin (OVA, grade V), bovine serum albumin (BSA, heat shock fraction), myoglobin from equine skeletal muscle (MYO), HB from bovine blood, LF from human milk, melittin from honey bee venom (MEL), histone f1 fraction from calf thymus (HF1) and SigmaFast OPD were purchased from Sigma-Aldrich (Madrid, Spain). Bovine casein (CAS, Hammarstein grade) was supplied by BDH Prolabo (VWR International Eurolab, Barcelona, Spain). Biotinylated PMX was obtained from Hycult Biotech (Uden, The Netherlands). Lysozyme from chicken egg white (LSZ) and deglycosylated avidin from hen egg white were procured from Fluka Analytical (Sigma-Aldrich, Steinheim, Germany). HRP-conjugated sheep anti-FITC was provided by Abd Serotec (Oxford, UK). Tween® 20 (TW2) and Triton® X-100 (TTX) were purchased from Merck KGaA (Darmstadt, Germany). Polystyrene microtiter plates were provided by Greiner Bio-One (Soria-Melguizo, Madrid, Spain). The synthetic peptide P3L (MCQCVQKYGTEFCKKRLA, 90% pure) from Anisakis simplex was synthesized at the Centro Nacional de Microbiología (Instituto de Salud Carlos III, Madrid, Spain). The synthetic protein corresponding to protein sMF6p/FhHDM-1 secreted by Fasciola hepatica (MF6p, gb CCA61804.1, 95% pure) was obtained from GeneCust Europe (Dudelange, Luxembourg).
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