The thymol (purity > 98.5%), LPS (Escherichia coli 055:B5, L2880), and dimethyl sulfoxide (DMSO, D4540) were obtained from Sigma-Aldrich (St. Louis, MO, USA). The mouse monoclonal anti-β-actin (AA128), HRP-labeled goat anti-rabbit IgG (H + L), and HRP-labeled goat anti-mouse IgG (H + L) antibodies (A0208, A0216) were purchased from Beyotime (Shanghai, China). The following antibodies were used in this work: anti-IκB α (4814, Cell Signaling Technology, Danvers, MA, USA), anti-phospho-NF-κB p65 (Ser536, 3033S, Cell Signaling Technology), anti-NLRP3 (AF2155, Beyotime), anti-IL-1β (AF7209, Beyotime), anti-beclin1 (ab231341, Abcam), anti-ATG7 (AA820, Beyotime), anti-LC3B (ab229327, Abcam, Cambridge, UK), anti-phospho-AMPK-α (Thr172, Cell Signaling Technology), anti-phospho-mTOR (Ser2448, D9C2, Cell Signaling Technology), anti-caspase-3 (WL02117, Wanleibio, Shenyang, China), and anti-cleaved caspase-9 (WL01838, Wanleibio).
Anti phospho mtor ser2448 d9c2
Manufactured by Cell Signaling Technology
Sourced in United Kingdom, United States
The Anti-phospho-mTOR (SER2448) (D9C2) is a rabbit monoclonal antibody that detects endogenous levels of mTOR protein when phosphorylated at serine 2448. This antibody is useful for the analysis of mTOR signaling.
Automatically generated - may contain errors
Lab products found in correlation
Anti pan akt, by Cell Signaling Technology (2 mentions)
β actin, by Cell Signaling Technology (2 mentions)
Anti phospho akt ser473, by Cell Signaling Technology (2 mentions)
Ab110413, by Abcam (2 mentions)
Anti phospho p44 42 map erk1 2, by Cell Signaling Technology (2 mentions)
Anti p44 42 mapk erk1 2 l34f12, by Cell Signaling Technology (2 mentions)
Anti klf10, by Abcam (2 mentions)
Anti mtor, by Cell Signaling Technology (2 mentions)
Anti phospho ampkα thr172, by Cell Signaling Technology (1 mentions)
Thymol, by Merck Group (1 mentions)
Hrp labeled goat anti mouse igg h l, by Beyotime (1 mentions)
Anti beclin1, by Abcam (1 mentions)
Anti iκb α 4814, by Cell Signaling Technology (1 mentions)
Anti cleaved caspase 9, by Wanlei (1 mentions)
Anti caspase 3, by Wanlei (1 mentions)
Powervision detection system, by Immunologic (1 mentions)
Anti mtor 7c10, by Cell Signaling Technology (1 mentions)
4 protocols using anti phospho mtor ser2448 d9c2
1
Molecular Mechanisms of Anti-inflammatory Effects
2
Immunoblotting of Signaling Pathways
3
Evaluating mTOR Expression in Acute Leukemia
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mTOR expression was assessed by immunocytochemistry (ICC) staining on bone marrow smears. We examined bone marrow samples from initial ALL diagnosis of all 31 patients and from relapse of 10 patients. One child developed acute myeloid leukaemia (AML) during maintenance chemotherapy of ALL. The myeloblasts of this patient were examined by ICC.
ICC stainings were carried out using primary rabbit monoclonal antibodies anti-mTOR (7C10) and anti-phospho–mTOR (Ser2448) (D9C2) (Cell Signaling, USA). After blocking endogenous peroxidase, bone marrow smears were incubated with diluted primary antibodies (anti-mTOR 1: 100; anti-p-mTOR 1: 50). Immunodetection was performed using a PowerVision detection system (ImmunoLogic, the Netherlands), and visualisation was carried out by DAB (3,3’-diaminobenzidine) (Daco, Denmark, USA). Stainings were evaluated by two cytologists independently, according to the following criteria: (0) no expression, (1+) mild to moderate staining intensity in all blasts or expression visible only in a limited number of blast cells, (2+) strong expression in all blast cells (Fig. 1 ).
ICC stainings were carried out using primary rabbit monoclonal antibodies anti-mTOR (7C10) and anti-phospho–mTOR (Ser2448) (D9C2) (Cell Signaling, USA). After blocking endogenous peroxidase, bone marrow smears were incubated with diluted primary antibodies (anti-mTOR 1: 100; anti-p-mTOR 1: 50). Immunodetection was performed using a PowerVision detection system (ImmunoLogic, the Netherlands), and visualisation was carried out by DAB (3,3’-diaminobenzidine) (Daco, Denmark, USA). Stainings were evaluated by two cytologists independently, according to the following criteria: (0) no expression, (1+) mild to moderate staining intensity in all blasts or expression visible only in a limited number of blast cells, (2+) strong expression in all blast cells (
4
Immunoblotting of Signaling Pathways
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Primary antibodies used were anti-phospho-Akt (Ser473; #4060, Cell Signaling), anti-pan-Akt (#2920, Cell Signaling), anti-phospho-P44/42 MAP(ERK1/2) (#9101, Cell Signaling), anti- P44/42 MAPK (ERK1/2) (L34F12) (#4696, Cell Signaling), anti-phospho-mTOR (SER2448 (D9C2), #5536, Cell Signaling), anti-mTOR (# 2972, Cell Signaling), anti-oxidative phosphorylation (OXPHOS) complexes I to V (#ab110413, Abcam), anti-KLF10 (#ab73537, Abcam), and β-actin (#4970, Cell Signaling).
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