Anti cd69 apcvio770

Manufactured by Miltenyi Biotec

Anti-CD69-APCVio770 is a fluorescence-labeled monoclonal antibody. It is designed to detect the expression of the CD69 cell surface antigen.

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Lab products found in correlation

Anti cd25 pe, by Miltenyi Biotec (1 mentions) Rbc lysis buffer, by Thermo Fisher Scientific (1 mentions) Anti cd3 viogreen, by Miltenyi Biotec (1 mentions) Flowlogic software, by Inivai Technologies (1 mentions) Anti cd25 apc, by Miltenyi Biotec (1 mentions) Celltrace cfse, by Thermo Fisher Scientific (1 mentions) Lsr 2 flow cytometer, by BD (1 mentions) Human il 7, by Miltenyi Biotec (1 mentions) Anti cd3 cd28 beads, by Thermo Fisher Scientific (1 mentions) Anti hla dr vioblue, by Miltenyi Biotec (1 mentions) Anti cd4 pe vio770, by Miltenyi Biotec (1 mentions)

2 protocols using anti cd69 apcvio770

Multiparameter Flow Cytometry Analysis

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Following 48 h, whole blood incubation, RBC lysis buffer (Thermo Fisher Scientific) was used to lyse the red blood cells. After washing with PBS, cells were stained with anti-CD3-Viogreen, anti-CD69-APCVio770, anti-CD95-PEVio770, anti-CD25-PE, anti-CD71-FITC, and anti-CD154-VioBlue (Miltenyi Biotec). The samples were measured after a final washing step, using a MaqsQuant10 analyzer. Before measurement, propidium iodide (PI) (Miltenyi Biotec) was added to assess viability. Analysis of the cell populations was performed with Flowlogic software (Inivai Technologies, Mentone VIC, Australia). For each time point the unstimulated samples were used to set the correct gating. The gating strategy is shown in Figure A1 in Appendix A.

in ‘t Veld A.E., Grievink H.W., Saghari M., Stuurman F.E., de Kam M.L., de Vries A.P., de Winter B.C., Burggraaf J., Cohen A.F, & Moerland M. (2019). Immunomonitoring of Tacrolimus in Healthy Volunteers: The First Step from PK- to PD-Based Therapeutic Drug Monitoring?. International Journal of Molecular Sciences, 20(19), 4710.

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T-cell Activation and Proliferation Assay

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Resting LT-CD4⁺ were stained with CellTrace CFSE (Invitrogen) according to the manufacturer’s protocol and cultured in presence of human IL-7 (20 ng/mL, Miltenyi Biotec) or activated with anti-CD3/CD28 beads (Thermo Scientific). Cells were sampled at different time points post-culture, washed in D-PBS by 5min centrifugation at 4 °C at 600g and stained for viability using the Fixable Live/Dead Aqua reagent (Thermo Scientific) according to the manufacturer protocol. Cells were then washed and stained with anti-CD4-PE-Vio770, anti-CD25-APC, anti-CD69-APC-Vio770 and anti-HLA-DR-VioBlue (Miltenyi Biotec) according to the manufacturer protocol. Acquisition was performed using a BD LSRII flow cytometer (Becton Dickinson). Results were analyzed using the FlowJo v10 Software (BD Biosciences).

Roux H.M., Figueiredo S., Sareoua L., Salmona M., Hamroune J., Adoux L., Migraine J., Hance A., Clavel F., Cheynier R, & Dutrieux J. (2023). DNA ultra-sensitive quantification, a technology for studying HIV unintegrated linear DNA. Cell Reports Methods, 3(4), 100443.

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