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2 protocols using manganese sulfate

1

In Vitro Digestion of Hericium erinaceus

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Hericium erinaceus was purchased from the Yongding Ecological Farming Family Farm, Ningqiang County, Hanzhong City, Shaanxi Province.
Saliva α amylase (2000 U/g) was obtained from Omega, Cheyenne, WY, USA. Pepsin (250 U/g), pancreatic enzymes (10 U/g), fructose oligosaccharide, salt acid, concentrated sulfuric acid, sodium hydroxide, sodium chloride, ferrous sulfate heptahydrate, manganese sulfate, zinc sulfate heptahydrate, cobalt chloride hexahydrate, nickel chloride, copper sulfate pentahydrate disodium phosphate, potassium chloride, urea, magnesium chloride hexahydrate, calcium chloride dihydrate, metaphosphate, sodium sulfate, acetic acid standard, propionic acid standard, and butyric acid standard were obtained from Sinopharm Group Chemical Reagent Co., Ltd., Shanghai, China. All other chemical reagents were of analytical grade and procured from Aladdin Chemical Reagent Co., Ltd. (Shanghai, China).
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2

Chloroquine Phosphate and Metal Salts

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Chloroquine phosphate (C18H26ClN3·2H3PO4, ≥ 99%, CAS No: 50-63-5) was purchased from Sigma-Aldrich. Iron (II) sulfate heptahydrate (FeSO4·7H2O, CAS No: 7782-63-0), copper (II) sulfate pentahydrate (CuSO4·5H2O, CAS No:7758-99-8), cobalt sulfate heptahydrate (CoSO4·7H2O, CAS No: 10026-24-1), magnesium sulfate heptahydrate (MgSO4·7H2O, CAS No: 7487-88-9), and manganese sulfate (MnSO4, CAS No: 7785-87-7), Sodium carbonate decahydrate (Na2CO3·10H2O) were purchased from Sinopharm Chemical Reagent Co., Ltd. Except for Chloroquine phosphate, all of the chemicals used were of analytical grade.
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