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Ivis lumina in vivo imaging system

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The IVIS Lumina in vivo imaging system is a bioluminescence and fluorescence imaging platform designed for small animal research. It provides sensitive detection and quantification of light emitted from luciferase-expressing cells or fluorescent probes within living subjects.

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Lab products found in correlation

Living image software version 4, by PerkinElmer (3 mentions) D luciferin potassium salt, by Fujifilm (2 mentions) Xenolight d luciferin k salt, by PerkinElmer (1 mentions) Luminescent atp detection assay kit, by Abcam (1 mentions) D luciferin potassium salt, by Beyotime (1 mentions) Coelenterazine h, by Promega (1 mentions) Her2 fc, by ACROBiosystems (1 mentions) Perfect block, by MoBiTec (1 mentions) P4543, by Merck Group (1 mentions) Nod cg prkdcscid il2rgtm1wjl szj mice, by Jackson ImmunoResearch (1 mentions) Luciferin, by PerkinElmer (1 mentions) D luciferin potassium salt, by PerkinElmer (1 mentions) Latheta lct 200, by Hitachi (1 mentions) Butorphanol tartrate, by Meiji Seika Pharma (1 mentions) D luciferin potassium salt, by GoldBio (1 mentions) Living image v 4, by PerkinElmer (1 mentions) D luciferin, by PerkinElmer (1 mentions) Matrigel basement membrane matrix, by Corning (1 mentions)

20 protocols using ivis lumina in vivo imaging system

1

Monitoring Metastasis Progression via Bioluminescence

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To observe the progression of metastatic disease in absence of primary tumor, weekly bioluminescence measurements were performed. Soluble Luciferin (XenoLight D-Luciferin - K+ Salt) (Perkin Elmer, 122799, Boston, MA) was injected intraperitoneally, and bioluminescence was measured using IVIS Lumina In Vivo Imaging System (Perkin Elmer, Boston, MA).
Banik D., Noonepalle S., Hadley M., Palmer E., Gracia-Hernandez M., Zevallos-Delgado C., Manhas N., Simonyan H., Young C.N., Popratiloff A., Chiappinelli K.B., Fernandes R., Sotomayor E.M, & Villagra A. (2020). HDAC6 plays a non-canonical role in the regulation of anti-tumor immune responses, dissemination, and invasiveness of breast cancer.. Cancer research, 80(17), 3649-3662.
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2

Luminescent ATP Detection in Astrocytes

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ATP concentration was detected with the use of the Luminescent ATP Detection Assay Kit (Abcam, Cambridge, UK) according to the manufactures guide lines. Briefly, cultured astrocytes were incubated with a detergent on a shaker for 5 minutes. After total cell lyses, substrate for luminescence detection was added and incubated for 5 minutes. Luminescence was analyzed by IVIS Lumina in vivo Imaging System (PerkinElmer).
Revuelta M., Elicegui A., Scheuer T., Endesfelder S., Bührer C., Moreno-Cugnon L., Matheu A, & Schmitz T. (2021). In vitro P38MAPK inhibition in aged astrocytes decreases reactive astrocytes, inflammation and increases nutritive capacity after oxygen-glucose deprivation. Aging (Albany NY), 13(5), 6346-6358.
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3

Bioluminescent Tumor Imaging in Mice

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Under the same sedation conditions, the mice were injected intraperitoneally with D-luciferin potassium salt (Wako Pure Chemical Corporation, Osaka, Japan) at 3 mg/mouse. Bioluminescent signals received during the 6 min acquisition time were quantified using the IVIS Lumina in vivo imaging system and Living Image Software Version 4.0 (Perkin Elmer, Waltham, MA, USA). Tumor area was measured using the region of interest (ROI) contour tool.
Yamamoto M., Taniguchi K., Masubuchi S., Tominaga T., Inomata Y., Miyamoto A., Ishizuka T.A., Murakami T., Osumi W., Hamamoto H., Tanaka K., Okuda J, & Uchiyama K. (2019). An In Vivo Mouse Model of Pelvic Recurrence of Human Colorectal Cancer. Scientific Reports, 9, 19630.
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4

In vivo CAR T Cell Efficacy Evaluation

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In vivo CAR T cell efficacy was evaluated using a xenograft model. Five days before T cell infusion, mice were intraperitoneally xenografted with 3 × 106 (100 μL) luciferase-expressing EBV LCL-lucH cells. After 5 days (on day 0), 5 × 106 T cells (300 μL) were injected intravenously per mouse. Four mice were injected with NT T cells, and five mice were injected with CD19 CAR T and MVR CAR T cells, respectively. The tumor burdens of the xenografted mice were determined on days 0, 7, 14, 21, and 28 by measuring luciferase activity with an IVIS Lumina in vivo imaging system (PerkinElmer, Inc., Waltham, MA, USA).
Han C., Sim S.J., Kim S.H., Singh R., Hwang S., Kim Y.I., Park S.H., Kim K.H., Lee D.G., Oh H.S., Lee S., Kim Y.H., Choi B.K, & Kwon B.S. (2018). Desensitized chimeric antigen receptor T cells selectively recognize target cells with enhanced antigen expression. Nature Communications, 9, 468.
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5

In Vivo Bioluminescent Tumor Imaging

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The mice were injected intraperitoneally with D‐luciferin potassium salt (Wako Pure Chemical Corporation) at 3 mg/mouse. Bioluminescent signals received during the 6‐minute acquisition time were quantified using the IVIS Lumina in vivo imaging system and Living Image Software Version 4.0 (Perkin Elmer). Tumor area was measured using the region of interest (ROI) contour tool.
Yamamoto M., Taniguchi K., Tominaga T., Shibata M., Inomata Y., Komura K., Osumi W., Hamamoto H., Tanaka K., Okuda J, & Uchiyama K. (2020). Evaluation of lymphatic flow pattern using indocyanine green fluorescence imaging in a highly metastatic mouse model. Cancer Science, 112(2), 774-780.
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6

Xenograft Mouse Model for Multiple Myeloma

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Xenograft mouse models of MM were established using NCG mice (NOD/ShiLtJGpt-Prkdcem26Cd52IL2rgem26Cd22/Gpt) aged 6 to 10 weeks. The mice received a tail vein injection of 2 × 106 MM.1S-mCherry.ffLuc or RPMI-8226-mCherry.ffLuc tumor cells in 0.1 mL PBS. On day 7 after tumor delivery, the mice were randomly divided into five groups. Subsequently, a dose of 5 × 106 UCAR-T cells in 0.1 mL sterile PBS was injected into the mice via the tail vein. Tumor growth was monitored using the IVIS Lumina In Vivo Imaging System (PerkinElmer). To visualize the tumors, the mice were injected with 150 mg/kg D-Luciferin potassium salt (cat# ST196, Beyotime) intraperitoneally and imaged with the IVIS. After 24 days of UCAR-T cells treatment, we collected peripheral blood of mice through the tail vein. The blood samples were incubated with BCMA-hFc (cat# 10620-H02H, SinoBiological), CD47-hFc or CD123-hFc (cat# 10518-H02H, SinoBiological) proteins, respectively, and stained with APC/Cy7 anti-human IgG Fc antibody (cat# 366912, Biolegend), and then analyzed by flow cytometry to determine the percentage of UCAR-T cells and mCherry cells.
Lu Q., Li H., Wu Z., Zhu Z., Zhang Z., Yang D, & Tong A. (2024). BCMA/CD47-directed universal CAR-T cells exhibit excellent antitumor activity in multiple myeloma. Journal of Nanobiotechnology, 22, 279.
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7

Establishing Pulmonary Metastasis Mouse Model

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In order to establish a mouse model of pulmonary metastasis, female Balb/c mice were intravenously injected with 5 × 105 4T1-Luciferase cells suspended in 100 μl of culture medium containing no FBS or antibiotics. Five days after inoculation, the mice were randomly assigned to one of three treatment groups (control, 25 mg/kg, or 50 mg/kg, n = 3/group). Then, the mice were intraperitoneally injected with different doses of BA every 2 days. At 5, 10, and 15 days post-treatment, the mice were anesthetized and intraperitoneally injected with 100 μl of D-luciferin (30 mg/ml in PBS). Then, the bioluminescence of the metastatic lung tumors was analyzed using an IVIS Lumina in vivo Imaging System (PerkinElmer, Inc., Waltham, MA, USA). At the end point of the experiment, lungs from each group were isolated, weighed, and the number of metastatic nodules (diameters of >3 and <3 mm) was counted.
Zeng A., Liang X., Zhu S., Liu C., Luo X., Zhang Q, & Song L. (2020). Baicalin, a Potent Inhibitor of NF-κB Signaling Pathway, Enhances Chemosensitivity of Breast Cancer Cells to Docetaxel and Inhibits Tumor Growth and Metastasis Both In Vitro and In Vivo. Frontiers in Pharmacology, 11, 879.
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8

Bioluminescence-based HER2 Binding Assay

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Bacteria expressing each RLuc-fused FLAP candidate were lysed by freeze-thaw treatment and sonication. After ultracentrifugation at 210,000 × g for 15 min, bioluminescence imaging of the supernatant was acquired for 30 s to determine the concentration of sample proteins based on the sample reaction with the RLuc substrate coelenterazine-h (10 ng/µl in PBS; Promega) using an IVIS Lumina in vivo imaging system (PerkinElmer, Waltham, MA, USA). Each BLI-ELISA was carried out at room temperature in 96-well black plates. The wells were first coated with HER2-Fc (50 ng/50 µl in PBS; ACRO Biosystems, Newark, DE, USA) overnight, blocked with PBS containing 2% Perfect-Block (MoBiTec, Göttingen, Germany) (PBS-PB) for 2 h, incubated with 100 µl of 1 µM sample proteins for 1 h, and washed three times with PBS containing 0.05% Tween-20 (PBS-T). After the addition of coelenterazine-h to each well, bioluminescence imaging was acquired for 30 s.
Kadonosono T., Yimchuen W., Ota Y., See K., Furuta T., Shiozawa T., Kitazawa M., Goto Y., Patil A., Kuchimaru T, & Kizaka-Kondoh S. (2020). Design Strategy to Create Antibody Mimetics Harbouring Immobilised Complementarity Determining Region Peptides for Practical Use. Scientific Reports, 10, 891.
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9

Xenograft and Metastatic Mouse Models

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The animal protocol for the experiments was approved by the NIDDK Animal Care and Use Committee. Eight to ten weeks old athymic nude mice and NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice (Jackson Laboratory, Bar Harbor, ME) were used to create subcutaneous xenograft and metastatic mouse models, respectively. For the metastatic model, the mice received a tail vein injection of FTC133 (160,000 cells) containing a linearized pGL4.51[luc2/CMV/Neo] vector (23 (link)). The tumor burden was monitored weekly in the mice using the IVIS Lumina In Vivo Imaging System (PerkinElmer) by injecting them with Luciferin (150mg Luciferin/kg body weight, PerkinElmer) 10 min before imaging. For the subcutaneous xenograft model, athymic nude mice were injected subcutaneously with FTC133, BCPAP, TT, or AR42J (500,000 cells). The tumor burden was monitored regularly by using a Vernier caliper. The mice were euthanized if the tumors exceeded 2cm in diameter, impeded movement, or if there were signs of breathing difficulty at any point in the study. The mice were grouped into control and treatment groups based on weight, gender and tumor burden. The mice were then treated with either VAC (300 µg/kg; Sigma Aldrich, P4543), decitabine (1 mg/kg), or saline (placebo) via intraperitoneal injections daily for 10 days followed by PET imaging or radionuclide therapy studies (Supplemental Figure 8).
Thakur S., Daley B., Millo C., Cochran C., Jacobson O., Lu H., Wang Z., Kiesewetter D.O., Chen X., Vasko V, & Klubo-Gwiezdzinska J. (2020). 177Lu-DOTA-EB-TATE, A Radiolabeled Analog of Somatostatin Receptor Type 2, for the Imaging and Treatment of Thyroid Cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 27(5), 1399-1409.
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10

Parasite Burden Determination in Malaria

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Parasitemia was determined by examination of Giemsa-stained thin blood smears every other day after infection. Parasite burden in mice infected with P. berghei–luc was assessed by an IVIS Lumina In Vivo Imaging System (PerkinElmer) after intraperitoneal injection of d-luciferin (100 mg/kg).
Fu Y., Ding Y., Wang Q., Zhu F., Tan Y., Lu X., Guo B., Zhang Q., Cao Y., Liu T., Cui L, & Xu W. (2020). Blood-stage malaria parasites manipulate host innate immune responses through the induction of sFGL2. Science Advances, 6(9), eaay9269.
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