Microwell dishes

Manufactured by MatTek

Sourced in United States

Microwell dishes are a type of laboratory equipment used to hold and culture cells in a controlled environment. These dishes feature a grid of small, circular wells that can accommodate small volumes of cell culture media and samples. They provide a consistent and standardized platform for various cell-based assays and experiments.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Glass-bottom microwell dish (8 citations) 35 mm glass bottom microwell dish (6 citations) 35-mm glass-bottomed microwell dish (5 citations) 20 mm glass bottom microwell dish (3 citations) 35mm glass bottom microwell petri dishes (3 citations) Coated 20-mm microwells of 35 mm petri dish (2 citations) Glass bottom microwell petri dish (2 citations) Pre-coated (poly-L-lysine) glass bottom microwell dishes (2 citations) 35 mm 14 mm glass microwell (no 1.5) glass bottom dishes (2 citations) Collagen pre-coated glass bottom microwell dishes (2 citations)

7 protocols using microwell dishes

Immunofluorescence Assay for SHCBP1 Nuclear Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

TMAs or cells cultured on 35 mm glass-bottomed Microwell Dishes (MatTek Corporation) were fixed with 4% paraformaldehyde for 10 min and subjected to permeabilization with 0.1% TritonX-100. After that, cells were incubated with 3% bovine serum for 1 h at room temperature and then with primary antibodies overnight at 4 °C, washed thrice in PBS and further incubated with the appropriate fluorescent-labeled secondary antibodies. Nuclei were counterstained with 4, 6-diamidino-2-phenylindole (DAPI) before mounting. Confocal fluorescence images were captured using a Zeiss LSM 880 laser microscope (×63 oil objective, Plan-Apochrom 1.4). The positivity of SHCBP1 in the nucleus was detected by SHCBP1 IF and was quantified using Image J V1.53c software. The mean fluorescence intensity (MFI) of SHCBP1 in the nucleus was quantified and the MFI > 10 was identified as the positive cells.

Shi W., Zhang G., Ma Z., Li L., Liu M., Qin L., Yu Z., Zhao L., Liu Y., Zhang X., Qin J., Ye H., Jiang X., Zhou H., Sun H, & Jiao Z. (2021). Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer. Nature Communications, 12, 2812.

+ Open protocol

+ Expand

Immunostaining and Expansion Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols

Punches were blocked in IF buffer (1% BSA; [Sigma-Aldrich; A9647] and 0.05% Tween-20 [Sigma-Aldrich; P9416] in 1× PBS) for 1–2 h at RT, and incubated with primary antibody diluted in IF buffer for 24 h at 4°C. Punches were washed in 1× PBS for 1–2 h and incubated with secondary antibody and DAPI (1:10,000 final dilution; Thermo Fisher Scientific; D1306) in IF buffer for 24 h at 4°C. The following primary antibodies were used for immunostaining: mouse anti-acetylated tubulin (Sigma-Aldrich; T7451) at 1:4,000, rabbit anti-β tubulin (Abcam; ab15568) at 1:250, rabbit anti-ARL13B (Abcam; ab83879) at 1:500, rabbit anti-Cep290 (Abcam; ab84870) at 1:600, and rabbit anti-Cep164 (Proteintech; 22227–1-AP) at 1:500. Secondary antibodies Alexa Fluor 488 anti-mouse (Thermo Fisher Scientific; A11029) and Alexa Fluor 555 anti-rabbit (Thermo Fisher Scientific; A21429) were used at a 1:800 dilution to label primary antibodies for 24 h. After immunostaining, the samples were expanded in deionized H₂O for 2 h at RT with deionized H₂O exchanged every 10 min and additionally overnight at 4°C. Prior to imaging, expanded punches were mounted in Rose chambers, Attofluor cell chambers, or glass-bottom Microwell dishes (MatTek; P35G-1.5-14-C).

Kong D., Sahabandu N., Sullenberger C., Vásquez-Limeta A., Luvsanjav D., Lukasik K, & Loncarek J. (2020). Prolonged mitosis results in structurally aberrant and over-elongated centrioles. The Journal of Cell Biology, 219(6), e201910019.

+ Open protocol

+ Expand

Transient Transfection of Mammalian Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chinese hamster ovary (CHO-K1) cells and African green monkey kidney epithelial cells (Vero E6 cells) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, PAA Laboratories GmbH, Austria) supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin (all PAA Laboratories GmbH, Pasching, Austria). Then, 24–48 h prior to imaging experiments, expression plasmids were introduced into pre-plated cells in 35-mm glass-bottom Microwell Dishes (MatTek Corporation, Ashlands, MA, USA) by Turbofect transfection (Thermo Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. All cell lines other than CHO-K1 (Figure S2: A549, HEK293T, MGLU-2-R [17 (link)], and MGN-2-R [17 (link)]) were seeded in a standard 12-well tissue culture plate (Greiner, Kremsmünster, Austria) on 18-mm glass cover slips (#1.5, Menzel, Thermo Scientific, Waltham, MA, USA). The cells were seeded at a density of 150.000 cells per well and cultivated in DMEM with 10% fetal calf serum (Sigma-Aldrich, Gillingham, UK). After 24 h, the cells were transfected with 1 µg plasmid DNA per well using jetOptimus (Polyplus, Illkirch, France) transfection reagent. The medium was changed after 6 h, and the cells were further incubated for 12–16 h, then fixed, stained, and mounted on microcopy glass slides (ProLong Gold, ThermoFisher, Waltham, MA, USA).

Welke R.W., Sperber H.S., Bergmann R., Koikkarah A., Menke L., Sieben C., Krüger D.H., Chiantia S., Herrmann A, & Schwarzer R. (2022). Characterization of Hantavirus N Protein Intracellular Dynamics and Localization. Viruses, 14(3), 457.

+ Open protocol

+ Expand

Glow-discharged grid preparation for EM

Check if the same lab product or an alternative is used in the 5 most similar protocols

Following glow-discharge, groups of three EM grids were placed on the glass bottom of microwell dishes (MatTek) with no overlap (Fig. 1 inset B). These microwell dishes were arranged in a 150mm x 15mm petri dish (CELLTREAT) and clustered around a central microwell dish containing distilled water to prevent the grids from drying. The grids were coated with 150 μL of 0.01% (w/v) poly-L-lysine solution (Sigma-Aldrich) for 2 h. The poly-L-lysine solution was removed by pipetting, followed by the addition of 150 μL of PBS. The addition and removal of PBS by pipetting (washing step) was done three times.

Jiang J., Cheong K.Y., Falkowski P.G, & Dai W. (2021). Integrating On-grid Immunogold Labeling and Cryo-Electron Tomography to Reveal Photosystem II Structure and Spatial Distribution in Thylakoid Membranes. Journal of structural biology, 213(3), 107746.

+ Open protocol

+ Expand

Microscopy Techniques for Visualizing Fixed and Live Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

To image fixed cells, cells were fixed, permeabilized (0.1% Triton-X100, 10 min), incubated with Abs (for immunofluorescence), and mounted on glass slides with ProLong Diamond antifade mountant (Molecular Probes). Cells were imaged with Zeiss 710 confocal microscope using 63x (COS-7) or 40x (myocytes) oil immersion objective. Fluorophores were sequentially excited by laser of 405 nm (DAPI and Hoechst dye), 488 nm (Alexa488 or GFP), 561 nm (Alexa568 or mCherry), and 633 (Alexa647). Pixel contents in regions of interest (ROIs), calculated as (mean pixel value in ROI –background)x(area of ROI), were used to calculate % pixel contents in different ROIs of a cell.
To image live cells, cells were plated on microwell dishes (MatTek), and transfected with cDNA or infected with adenovirus. After staining nuclei with Hoechst dye, COS-7 cells were imaged in phenol-red free, 25 mM HEPES DMEM supplemented with 10% FCS at 37°C. Live myocytes were imaged in normal Tyrode’s supplemented with 10% FCS and 10 uM blebbistatin (to prevent contractile activity) at 37°C. In fluorescence loss in photobleaching (FLIP) experiments, mean pixel values in delineated ROIs were reported by Zeiss software and exported to Excel for processing.

Murthy A., Workman S., Jiang M., Hu J., Sifa I., Bernas T., Tang W., Deschenes I, & Tseng G.N. (2019). Dynamic Palmitoylation Regulates Trafficking of K Channel Interacting Protein 2 (KChIP2) across Multiple Subcellular Compartments in Cardiac Myocytes. Journal of molecular and cellular cardiology, 135, 1-9.

+ Open protocol

+ Expand

Visualizing CAR-T Cell Viability in Fibrin Gel

Check if the same lab product or an alternative is used in the 5 most similar protocols

To visualize the distribution of cells in the fibrin gel (AF488-fibrin), CAR-T cells were labeled with Hoechst 33254. CAR-T cells (1 × 10⁶ cells) were polymerized in the hydrogel and immersed in culture medium using MatTek microwell dishes and analyzed by confocal microscope. To qualitatively and quantitatively characterize the viability and proliferation of CAR-T cells in the fibrin gel matrix and assess the morphology of T cells interacting with the matrix, we used a Zeiss LSM 710 inverted confocal microscope with differential interference contrast (DIC) controlled by Zeiss ZEN 2011 software. CAR-T cells (1 × 10⁶) were polymerized in the fibrin gel and placed in culture medium. Cells in the fibrin gel were analyzed for viability on days 1 to 5, in which cells were stained by immersing the hydrogel in the LIVE/DEAD Viability/Cytotoxicity Kit for 10 min. Samples were analyzed with a confocal microscope, where live cells were labeled with green fluorescein and dead cells with red fluorescein. The Imaris image visualization and analysis software (Oxford Instruments) was used on at least three different Z-stack slices to quantify the number of live and dead cells for each sample.

Ogunnaike E.A., Valdivia A., Yazdimamaghani M., Leon E., Nandi S., Hudson H., Du H., Khagi S., Gu Z., Savoldo B., Ligler F.S., Hingtgen S, & Dotti G. (2021). Fibrin gel enhances the antitumor effects of chimeric antigen receptor T cells in glioblastoma. Science Advances, 7(41), eabg5841.

+ Open protocol

+ Expand

Substrate Rigidity and Cell Adhesion

Check if the same lab product or an alternative is used in the 5 most similar protocols

PDMS substrates of varying rigidities were prepared using a Sylgard 184 silicone elastomer kit (Dow Corning, Midland, MI; Prager-Khoutorsky et al., 2011 (link)). The silicone elastomer component was mixed with the curing agent, degassed, and spin-coated at 2000 rpm for 2 min on glass-bottom Microwell dishes (MatTek, Ashland, MA). Subsequently, cross-linking of the elastomer was carried out at 70°C for 4 h. The elastomer-to-curing agent ratios of 10:1 and 75:1 corresponded to Young's moduli 2 MPa and 5 kPa, respectively (Prager-Khoutorsky et al., 2011 (link)). Dishes with a layer of PDMS were functionalized with 10 μg/ml of vitronectin overnight at 4°C, and cells were plated after the surface was washed two times with PBS.

Ribeiro S.A., D'Ambrosio M.V, & Vale R.D. (2014). Induction of focal adhesions and motility in Drosophila S2 cells. Molecular Biology of the Cell, 25(24), 3861-3869.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!