Cell culture grade plastic materials

All oligonucleotides and dual-labeled probes used were purchased from Sigma, Deisenhofen, Germany. Complete EDTA-free protease and a phosphatase inhibitor cocktail were obtained from Roche Diagnostics, Mannheim, Germany. All cell culture grade plastic materials were obtained from Greiner, Solingen, Germany. The High-Capacity cDNA Reverse Transcription Kit was purchased from Applied Biosystems, Darmstadt, Germany. The M-MuLV reverse transcriptase and the Taq 2x Master Mix was obtained from New England Biolabs, Ipswich, USA. The AMPLIFYME SG Universal Mix was obtained from 7Bioscience, Neuenburg am Rhein, Germany.

Trypsin, glutamine, sodium pyruvate, and all oligonucleotides were purchased from Sigma-Aldrich, Deisenhofen, Germany. All cell-culture-grade plastic materials were obtained from Greiner, Solingen, Germany. FCS and DMEM were purchased from PAN-Systems, Nürnberg, Germany. Restriction enzymes, polynucleotide kinase (PNK), calf intestinal alkaline phosphatase (CIAP), Taq DNA polymerase, Klenow DNA polymerase, dNTPs, and NTPs were purchased from New England Biolabs, Frankfurt a.M., Germany. T7 and T3 RNA and DNase I polymerase were obtained from Roche Diagnostics, Mannheim, Germany. pCR-Script was obtained from Agilent Technologies, Waldbronn, Germany. The plasmids pGL3control_TNF3UTR and pGL3control_TNF3UTRAUdel were kind gifts from Dr. W.F.C. Rigby [16 (link)].