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Igg total human elisa kit

Manufactured by Thermo Fisher Scientific
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The IgG (Total) Human ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of total human immunoglobulin G (IgG) levels in various sample types.

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Lab products found in correlation

Gm csf human elisa kit, by Thermo Fisher Scientific (2 mentions) Glutamine penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Recombinant human il 33 protein, by R&D Systems (1 mentions) Ficoll paque density gradient, by GE Healthcare (1 mentions) Il 9 elisa kit, by BioLegend (1 mentions) 1420 multilabel counter, by PerkinElmer (1 mentions) Gm csf mouse elisa kit, by Thermo Fisher Scientific (1 mentions) Cedex bio analyzer, by Roche (1 mentions) V plex plus human gm csf kit, by Mesoscale (1 mentions) Immunocap 100, by Thermo Fisher Scientific (1 mentions)

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ELISAs (35 citations)

13 protocols using igg total human elisa kit

1

IgG Induction by IL-33 and anti-IgM/CD40L

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Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized peripheral blood using Ficoll-Paque density gradient (GE Healthcare). PBMCs were washed with phosphate-buffered saline (PBS) and cultured in RPMI 1640 medium with 10 % FCS and 1 % glutamine/penicillin/streptomycin (Life Technologies). Recombinant human IL-33 protein was purchased from R&D Systems. Anti-human IgM antibody was purchased from eBioscience. Recombinant human CD40 Ligand (CD40L) was purchased from Life Technologies. For in vitro induction of anti-RBC IgG antibody, PBMCs (1 × 106/ml) were cultured with anti-IgM (10 μg/ml) and CD40L (10 ng/ml) in the presence or absence of IL-33 (0–20 ng/ml). Six days later, the supernatants were collected and assayed for IgG antibodies with Human IgG total ELISA kit (eBioscience).
Bu X., Zhang T., Wang C., Ren T, & Wen Z. (2015). IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production. Journal of Translational Medicine, 13, 381.
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2

Quantifying IgG and IgG3 Production

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Total IgG production was measured in coculture supernatants using Human IgG total ELISA kit, and IgG3 production was measured with IgG Subclass Human ELISA kit (both eBioscience, Thermo Fisher Scientific).
Louis K., Bailly E., Macedo C., Lau L., Ramaswami B., Chang A., Chandran U., Landsittel D., Gu X., Chalasani G., Zeevi A., Randhawa P., Singh H., Lefaucheur C, & Metes D. (2021). T-bet+CD27+CD21– B cells poised for plasma cell differentiation during antibody-mediated rejection of kidney transplants. JCI Insight, 6(12), e148881.
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3

IgG Levels Determination by ELISA

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Total human IgG levels were determined following the manufacturer’s instructions in the Human IgG Total ELISA Kit (Invitrogen).
Carroll T.D., Wong T., Morris M.K., Di Germanio C., Ma Z.M., Stone M., Ball E., Fritts L., Rustagi A., Simmons G., Busch M, & Miller C.J. (2023). Administration of vaccine-boosted COVID-19 convalescent plasma to SARS-CoV-2 infected hamsters decreases virus replication in lungs and hastens resolution of the infection despite transiently enhancing disease and lung pathology. bioRxiv.
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4

Quantifying Serum Immunoglobulins and Cytokine Levels

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Total Human IgG and IgM concentration was quantified using Human IgG Total ELISA Kit and Human IgM ELISA kit (Invitrogen, USA) respectively, according to the manufacturer’s protocol. Levels of soluble IL-9 was assessed in serum by IL-9 ELISA kit (Biolegend) as per the manufacturer’s protocol.
Chakraborty S., Kumari R., Gupta D., Makharia G.K., Ahuja V., Kumar P, & Mitra D.K. (2023). Interleukin-9 rescues class switching of Memory B cells derived from Common variable immunodeficiency patients. Clinical immunology (Orlando, Fla.), 254, 109697.
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5

Quantification of Total IgG in Supernatant

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Human IgG total ELISA Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA) was used to measure total IgG concentration in cell culture supernatant, according to the manufacturer’s instruction. Optical density were measured at 450 nm using a 1420 multilabel counter (PerkinElmer life and analytical sciences, Boston, MA).
Li Y., Guptill J.T., Russo M.A., Howard JF J.r., Massey J.M., Juel V.C., Hobson-Webb L.D., Emmett D., Chopra M., Raja S., Liu W, & Yi J.S. (2020). Imbalance in T Follicular Helper Cells Producing IL-17 Promotes Pro-inflammatory Responses in MuSK Antibody Positive Myasthenia Gravis. Journal of neuroimmunology, 345, 577279.
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6

Recombinant Protein Release Analysis

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For recombinant protein release analysis, cells were cultured in a T75 cm2 culture flask. Once 75% confluency was reached, cells were washed with HBSS and incubated in 5 mL of fresh GM for 2 h at 37°C in a standard incubator. The medium was then collected for quantification and cells were counted using an automated cell counter. Three ELISA kits were used for supernatant analysis: a GM-CSF Human ELISA Kit with a lower limit of quantification at 7.8 pg/mL (Thermo Fisher Scientific, KHC2011), a GM-CSF Mouse ELISA Kit (Thermo Fisher Scientific, BMS612), and an IgG (Total) Human ELISA Kit (Thermo Fisher Scientific, BMS2091). All kits were used according to the manufacturers’ instructions. Results were reported in pg/cell/day.
Lathuiliere A., Vernet R., Charrier E., Urwyler M., Von Rohr O., Belkouch M.C., Saingier V., Bouvarel T., Guillarme D., Engel A., Salmon P., Laumonier T., Grogg J, & Mach N. (2022). Immortalized human myoblast cell lines for the delivery of therapeutic proteins using encapsulated cell technology. Molecular Therapy. Methods & Clinical Development, 26, 441-458.
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7

Serum IgG Quantification Post-Transplant

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Serum was aliquoted after blood collection at week 16-18 post-transplant. Titration of human IgG was performed using IgG (Total) Human ELISA Kit (BMS2091; ThermoFisher Scientific) according to manufacturer’s instructions.
Brault J., Liu T., Liu S., Lawson A., Choi U., Kozhushko N., Bzhilyanskaya V., Pavel-Dinu M., Meis R.J., Eckhaus M.A., Burkett S.S., Bosticardo M., Kleinstiver B.P., Notarangelo L.D., Lazzarotto C.R., Tsai S.Q., Wu X., Dahl G.A., Porteus M.H., Malech H.L, & De Ravin S.S. (2023). CRISPR-Cas9-AAV versus lentivector transduction for genome modification of X-linked severe combined immunodeficiency hematopoietic stem cells. Frontiers in Immunology, 13, 1067417.
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8

Optimizing Rituximab Production in CHO Cells

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Samples were collected from days 1,
3, 5, 7, and 8 of a fed-batch
cultivation carried out in a 4 L benchtop bioreactor (Belach Bioteknik,
Stockholm, Sweden). The cells were Rituximab-producing Chinese hamster
ovary cells (TurboCellTM, kindly provided by Rentschler Biopharma,
Laupheim, Germany). The base and feed media were chemically defined
proprietary media without animal-derived components. CHO HCP concentrations
were quantified using a CHO HCP ELISA kit, 3G (F550) from Cygnus Technologies.
Rituximab concentrations were quantified using an IgG (total) Human
ELISA kit (BMS2091) from Thermo Fisher Scientific and purified Rituximab
as internal standard. For both ELISA kits, all samples and calibration
wells were measured in duplicate according to the instructions from
the suppliers. LDH concentrations were determined with triplicate
measurements from centrifuged (180g for 5 min) samples
with Cedex Bio Analyzer (Roche Diagnostics Deutschland GmbH, Mannheim,
Germany).
Pinto I.F., Soares R.R., Mäkinen M.E., Chotteau V, & Russom A. (2021). Multiplexed Microfluidic Cartridge for At-Line Protein Monitoring in Mammalian Cell Culture Processes for Biopharmaceutical Production. ACS Sensors, 6(3), 842-851.
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9

Quantifying Secreted IgG by ELISA

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IgG secreted into the culture supernatant was quantitated by sandwich ELISA using an IgG (Total) Human ELISA Kit (Thermo Fisher) according to the manufacturer’s instructions.
Hoshino Y., Noto D., Sano S., Tomizawa Y., Yokoyama K., Hattori N, & Miyake S. (2022). Dysregulated B cell differentiation towards antibody-secreting cells in neuromyelitis optica spectrum disorder. Journal of Neuroinflammation, 19, 6.
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10

Quantitative IgG and IgM ELISA

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Total IgG and IgM levels in culture supernatants were determined using an IgG total human ELISA kit (Thermo Fisher Scientific) and an ELISA Flex: Human IgM (ALP) kit (MABTECH, Nacka Strand, Sweden), respectively. For IgM detection, pNPP (MABTECH, Nacka Strand, Sweden) was used as the substrate for the ALP signal detection. Both assays were performed in accordance with the manufacturer's instructions.
Sano S., Yoshikawa S., Hoshino Y., Tomizawa Y., Hattori N, & Miyake S. (2024). Establishment of novel cell lines that maintain the features of B cells derived from patients with neuromyelitis optica spectrum disorder. Immunological medicine, 47(3).
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